Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD.
Identifieur interne : 000350 ( PubMed/Corpus ); précédent : 000349; suivant : 000351Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD.
Auteurs : Nirmal K. Roy ; Nichole Walker ; R Christopher Chambers ; Isaac WirginSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 1879-1514 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Cytochrome P-450 CYP1A1.
- chemical , metabolism : Biomarkers, Cytochrome P-450 CYP1A1, RNA, Messenger.
- metabolism : Fishes.
- chemical , toxicity : Polychlorinated Biphenyls, Polychlorinated Dibenzodioxins, Water Pollutants, Chemical.
- Animals, Atlantic Ocean.
Abstract
The AHR pathway activates transcription of CYP1A and mediates most toxic responses from exposure to halogenated aromatic hydrocarbon contaminants such as PCBs and PCDD/Fs. Therefore, expression of CYP1A is predictive of most higher level toxic responses from these chemicals. To date, no study had developed an assay to quantify CYP1A expression in any sturgeon species. We addressed this deficiency by partially characterizing CYP1A in Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and shortnose sturgeon (Acipenser brevirostrum) and then used derived sturgeon sequences to develop reverse transcriptase (RT)-PCR assays to quantify CYP1A mRNA expression in TCDD and PCB126 treated early life-stages of both species. Phylogenetic analysis of CYP1A, CYP1B, CYP1C and CYP3A deduced amino acid sequences from other fishes and sturgeons revealed that our putative Atlantic sturgeon and shortnose sturgeon CYP1A sequences most closely clustered with previously derived CYP1A sequences. We then used semi-quantitative and real-time RT-PCR to measure CYP1A mRNA levels in newly hatched Atlantic sturgeon and shortnose sturgeon larvae that were exposed to graded doses of waterborne PCB126 (0.01-1000 parts per billion (ppb)) and TCDD (0.001-10 ppb). We initially observed significant induction of CYP1A mRNA compared to vehicle control at the lowest doses of PCB126 and TCDD used, 0.01 ppb and 0.001 ppb, respectively. Significant induction was observed at all doses of both chemicals although lower expression was seen at the highest doses. We also compared CYP1A expression among tissues of i.p. injected shortnose sturgeon and found significant inducibility in heart, intestine, and liver, but not in blood, gill, or pectoral fin clips. For the first time, our results indicate that young life-stages of sturgeons are sensitive to AHR ligands at environmentally relevant concentrations, however, it is yet to be determined if induction of CYP1A can be used as a biomarker in environmental biomonitoring.
DOI: 10.1016/j.aquatox.2011.03.009
PubMed: 21543048
Links to Exploration step
pubmed:21543048Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD.</title><author><name sortKey="Roy, Nirmal K" sort="Roy, Nirmal K" uniqKey="Roy N" first="Nirmal K" last="Roy">Nirmal K. Roy</name><affiliation><nlm:affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Walker, Nichole" sort="Walker, Nichole" uniqKey="Walker N" first="Nichole" last="Walker">Nichole Walker</name></author><author><name sortKey="Chambers, R Christopher" sort="Chambers, R Christopher" uniqKey="Chambers R" first="R Christopher" last="Chambers">R Christopher Chambers</name></author><author><name sortKey="Wirgin, Isaac" sort="Wirgin, Isaac" uniqKey="Wirgin I" first="Isaac" last="Wirgin">Isaac Wirgin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21543048</idno><idno type="pmid">21543048</idno><idno type="doi">10.1016/j.aquatox.2011.03.009</idno><idno type="wicri:Area/PubMed/Corpus">000350</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000350</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD.</title><author><name sortKey="Roy, Nirmal K" sort="Roy, Nirmal K" uniqKey="Roy N" first="Nirmal K" last="Roy">Nirmal K. Roy</name><affiliation><nlm:affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Walker, Nichole" sort="Walker, Nichole" uniqKey="Walker N" first="Nichole" last="Walker">Nichole Walker</name></author><author><name sortKey="Chambers, R Christopher" sort="Chambers, R Christopher" uniqKey="Chambers R" first="R Christopher" last="Chambers">R Christopher Chambers</name></author><author><name sortKey="Wirgin, Isaac" sort="Wirgin, Isaac" uniqKey="Wirgin I" first="Isaac" last="Wirgin">Isaac Wirgin</name></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="eISSN">1879-1514</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Atlantic Ocean</term><term>Biomarkers (metabolism)</term><term>Cytochrome P-450 CYP1A1 (genetics)</term><term>Cytochrome P-450 CYP1A1 (metabolism)</term><term>Fishes (metabolism)</term><term>Polychlorinated Biphenyls (toxicity)</term><term>Polychlorinated Dibenzodioxins (toxicity)</term><term>RNA, Messenger (metabolism)</term><term>Water Pollutants, Chemical (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytochrome P-450 CYP1A1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biomarkers</term><term>Cytochrome P-450 CYP1A1</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Polychlorinated Biphenyls</term><term>Polychlorinated Dibenzodioxins</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Atlantic Ocean</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The AHR pathway activates transcription of CYP1A and mediates most toxic responses from exposure to halogenated aromatic hydrocarbon contaminants such as PCBs and PCDD/Fs. Therefore, expression of CYP1A is predictive of most higher level toxic responses from these chemicals. To date, no study had developed an assay to quantify CYP1A expression in any sturgeon species. We addressed this deficiency by partially characterizing CYP1A in Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and shortnose sturgeon (Acipenser brevirostrum) and then used derived sturgeon sequences to develop reverse transcriptase (RT)-PCR assays to quantify CYP1A mRNA expression in TCDD and PCB126 treated early life-stages of both species. Phylogenetic analysis of CYP1A, CYP1B, CYP1C and CYP3A deduced amino acid sequences from other fishes and sturgeons revealed that our putative Atlantic sturgeon and shortnose sturgeon CYP1A sequences most closely clustered with previously derived CYP1A sequences. We then used semi-quantitative and real-time RT-PCR to measure CYP1A mRNA levels in newly hatched Atlantic sturgeon and shortnose sturgeon larvae that were exposed to graded doses of waterborne PCB126 (0.01-1000 parts per billion (ppb)) and TCDD (0.001-10 ppb). We initially observed significant induction of CYP1A mRNA compared to vehicle control at the lowest doses of PCB126 and TCDD used, 0.01 ppb and 0.001 ppb, respectively. Significant induction was observed at all doses of both chemicals although lower expression was seen at the highest doses. We also compared CYP1A expression among tissues of i.p. injected shortnose sturgeon and found significant inducibility in heart, intestine, and liver, but not in blood, gill, or pectoral fin clips. For the first time, our results indicate that young life-stages of sturgeons are sensitive to AHR ligands at environmentally relevant concentrations, however, it is yet to be determined if induction of CYP1A can be used as a biomarker in environmental biomonitoring.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21543048</PMID><DateCreated><Year>2011</Year><Month>06</Month><Day>13</Day></DateCreated><DateCompleted><Year>2011</Year><Month>09</Month><Day>19</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1514</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>1-2</Issue><PubDate><Year>2011</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Aquatic toxicology (Amsterdam, Netherlands)</Title><ISOAbbreviation>Aquat. Toxicol.</ISOAbbreviation></Journal><ArticleTitle>Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD.</ArticleTitle><Pagination><MedlinePgn>23-31</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.aquatox.2011.03.009</ELocationID><Abstract><AbstractText>The AHR pathway activates transcription of CYP1A and mediates most toxic responses from exposure to halogenated aromatic hydrocarbon contaminants such as PCBs and PCDD/Fs. Therefore, expression of CYP1A is predictive of most higher level toxic responses from these chemicals. To date, no study had developed an assay to quantify CYP1A expression in any sturgeon species. We addressed this deficiency by partially characterizing CYP1A in Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and shortnose sturgeon (Acipenser brevirostrum) and then used derived sturgeon sequences to develop reverse transcriptase (RT)-PCR assays to quantify CYP1A mRNA expression in TCDD and PCB126 treated early life-stages of both species. Phylogenetic analysis of CYP1A, CYP1B, CYP1C and CYP3A deduced amino acid sequences from other fishes and sturgeons revealed that our putative Atlantic sturgeon and shortnose sturgeon CYP1A sequences most closely clustered with previously derived CYP1A sequences. We then used semi-quantitative and real-time RT-PCR to measure CYP1A mRNA levels in newly hatched Atlantic sturgeon and shortnose sturgeon larvae that were exposed to graded doses of waterborne PCB126 (0.01-1000 parts per billion (ppb)) and TCDD (0.001-10 ppb). We initially observed significant induction of CYP1A mRNA compared to vehicle control at the lowest doses of PCB126 and TCDD used, 0.01 ppb and 0.001 ppb, respectively. Significant induction was observed at all doses of both chemicals although lower expression was seen at the highest doses. We also compared CYP1A expression among tissues of i.p. injected shortnose sturgeon and found significant inducibility in heart, intestine, and liver, but not in blood, gill, or pectoral fin clips. For the first time, our results indicate that young life-stages of sturgeons are sensitive to AHR ligands at environmentally relevant concentrations, however, it is yet to be determined if induction of CYP1A can be used as a biomarker in environmental biomonitoring.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Roy</LastName><ForeName>Nirmal K</ForeName><Initials>NK</Initials><AffiliationInfo><Affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Walker</LastName><ForeName>Nichole</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Chambers</LastName><ForeName>R Christopher</ForeName><Initials>RC</Initials></Author><Author ValidYN="Y"><LastName>Wirgin</LastName><ForeName>Isaac</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P30 ES000260</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>ES00260</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P42 ES010344-05</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P42 ES010344</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>ES10344</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>03</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Aquat Toxicol</MedlineTA><NlmUniqueID>8500246</NlmUniqueID><ISSNLinking>0166-445X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000072317">Polychlorinated Dibenzodioxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014874">Water Pollutants, Chemical</NameOfSubstance></Chemical><Chemical><RegistryNumber>DFC2HB4I0K</RegistryNumber><NameOfSubstance UI="D011078">Polychlorinated Biphenyls</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.14.1</RegistryNumber><NameOfSubstance UI="D019363">Cytochrome P-450 CYP1A1</NameOfSubstance></Chemical><Chemical><RegistryNumber>TSH69IA9XF</RegistryNumber><NameOfSubstance UI="C023035">3,4,5,3',4'-pentachlorobiphenyl</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Curr Drug Metab. 2001 Jun;2(2):149-64</RefSource><PMID Version="1">11469723</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochemistry. 2007 Jan 23;46(3):696-708</RefSource><PMID Version="1">17223691</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Toxicol Chem. 2002 Aug;21(8):1618-27</RefSource><PMID Version="1">12152761</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochem Biophys Res Commun. 2003 May 2;304(2):223-8</RefSource><PMID Version="1">12711302</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Sci Technol. 2004 Feb 15;38(4):976-83</RefSource><PMID Version="1">14998007</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Toxicol Appl Pharmacol. 2004 Apr 15;196(2):191-205</RefSource><PMID Version="1">15081266</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Pharmacol. 2004 Sep;66(3):512-21</RefSource><PMID Version="1">15322242</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13743-8</RefSource><PMID Version="1">9391097</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Toxicol Appl Pharmacol. 1998 Jan;148(1):24-34</RefSource><PMID Version="1">9465260</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998 Nov;121(1-3):23-53</RefSource><PMID Version="1">9972449</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Sci Technol. 2008 Oct 1;42(19):7535-41</RefSource><PMID Version="1">18939598</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Aquat Toxicol. 2010 Aug 15;99(2):232-40</RefSource><PMID Version="1">20605646</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2011 Mar 11;331(6022):1322-5</RefSource><PMID Version="1">21330491</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Aquat Toxicol. 2006 Mar 10;76(3-4):306-20</RefSource><PMID Version="1">16313980</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Methods. 2001 Dec;25(4):402-8</RefSource><PMID Version="1">11846609</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001267" MajorTopicYN="N">Atlantic Ocean</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019363" MajorTopicYN="N">Cytochrome P-450 CYP1A1</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011078" MajorTopicYN="N">Polychlorinated Biphenyls</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000072317" MajorTopicYN="N">Polychlorinated Dibenzodioxins</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014874" MajorTopicYN="N">Water Pollutants, Chemical</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">NIHMS293972</OtherID><OtherID Source="NLM">PMC3119503</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>02</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>03</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>03</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>5</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>5</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21543048</ArticleId><ArticleId IdType="pii">S0166-445X(11)00088-9</ArticleId><ArticleId IdType="doi">10.1016/j.aquatox.2011.03.009</ArticleId><ArticleId IdType="pmc">PMC3119503</ArticleId><ArticleId IdType="mid">NIHMS293972</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000350 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000350 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:21543048
|texte= Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:21543048" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |