Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis.
Identifieur interne : 000283 ( PubMed/Corpus ); précédent : 000282; suivant : 000284Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis.
Auteurs : Huan Ye ; Xihua Chen ; Qiwei Wei ; Li Zhou ; Tao Liu ; Jianfang Gui ; Chuangju Li ; Hong CaoSource :
- Gene [ 1879-0038 ] ; 2012.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, Cloning, Molecular, DNA Primers, DNA, Complementary, Fishes, Fluorescent Antibody Technique, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, RNA, Messenger (genetics), RNA-Binding Proteins (genetics), Sequence Homology, Amino Acid.
- MESH :
- chemical , genetics : RNA, Messenger, RNA-Binding Proteins.
- chemical : DNA Primers, DNA, Complementary.
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, Cloning, Molecular, Fishes, Fluorescent Antibody Technique, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Homology, Amino Acid.
Abstract
The nanos gene family was essential for germ line development in diverse organisms. In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species.
DOI: 10.1016/j.gene.2012.09.005
PubMed: 23010197
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pubmed:23010197Le document en format XML
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In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23010197</PMID><DateCreated><Year>2012</Year><Month>11</Month><Day>02</Day></DateCreated><DateCompleted><Year>2013</Year><Month>01</Month><Day>15</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0038</ISSN><JournalIssue CitedMedium="Internet"><Volume>511</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Dec</Month><Day>15</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis.</ArticleTitle><Pagination><MedlinePgn>285-92</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.gene.2012.09.005</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0378-1119(12)01071-2</ELocationID><Abstract><AbstractText>The nanos gene family was essential for germ line development in diverse organisms. In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Huan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xihua</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Qiwei</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Tao</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Gui</LastName><ForeName>Jianfang</ForeName><Initials>J</Initials></Author><Author 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