Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus).
Identifieur interne : 000276 ( PubMed/Corpus ); précédent : 000275; suivant : 000277Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus).
Auteurs : A. Shaliutina ; M. Hulak ; P. Li ; M. Sulc ; B. Dzyuba ; O. LinhartSource :
- Reproduction in domestic animals = Zuchthygiene [ 1439-0531 ] ; 2013.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Fish Proteins.
- physiology : Fishes, Gene Expression Regulation, Semen, Spermatozoa.
- Animals, Electrophoresis, Polyacrylamide Gel, Male, Time Factors.
Abstract
Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed. Screening of these spots by mass spectrometric analysis showed positive results for spot 10. Direct comparison across public databases revealed sequence similarity with two hypothetical proteins, MCAG_00854 and IscW_ISCW011489. Differences in the seminal plasma protein fractions were found at the third stripping compared to the first two. It is hypothesized that these extra proteins after the third collection could be involved in some step of intracellular mechanism which is responsible for regulating of spermatozoa motility. However, protein identification revealed no significant distinction for any protein spot and protein sequences available in public databases. These results highlighted the need for a complete genome sequences for sturgeons.
DOI: 10.1111/j.1439-0531.2012.02118.x
PubMed: 22747924
Links to Exploration step
pubmed:22747924Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus).</title>
<author><name sortKey="Shaliutina, A" sort="Shaliutina, A" uniqKey="Shaliutina A" first="A" last="Shaliutina">A. Shaliutina</name>
<affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M" last="Hulak">M. Hulak</name>
</author>
<author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P" last="Li">P. Li</name>
</author>
<author><name sortKey="Sulc, M" sort="Sulc, M" uniqKey="Sulc M" first="M" last="Sulc">M. Sulc</name>
</author>
<author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name>
</author>
<author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:22747924</idno>
<idno type="pmid">22747924</idno>
<idno type="doi">10.1111/j.1439-0531.2012.02118.x</idno>
<idno type="wicri:Area/PubMed/Corpus">000276</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000276</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus).</title>
<author><name sortKey="Shaliutina, A" sort="Shaliutina, A" uniqKey="Shaliutina A" first="A" last="Shaliutina">A. Shaliutina</name>
<affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M" last="Hulak">M. Hulak</name>
</author>
<author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P" last="Li">P. Li</name>
</author>
<author><name sortKey="Sulc, M" sort="Sulc, M" uniqKey="Sulc M" first="M" last="Sulc">M. Sulc</name>
</author>
<author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name>
</author>
<author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name>
</author>
</analytic>
<series><title level="j">Reproduction in domestic animals = Zuchthygiene</title>
<idno type="eISSN">1439-0531</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Fish Proteins (physiology)</term>
<term>Fishes (physiology)</term>
<term>Gene Expression Regulation (physiology)</term>
<term>Male</term>
<term>Semen (physiology)</term>
<term>Spermatozoa (physiology)</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Fish Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term>
<term>Gene Expression Regulation</term>
<term>Semen</term>
<term>Spermatozoa</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Male</term>
<term>Time Factors</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed. Screening of these spots by mass spectrometric analysis showed positive results for spot 10. Direct comparison across public databases revealed sequence similarity with two hypothetical proteins, MCAG_00854 and IscW_ISCW011489. Differences in the seminal plasma protein fractions were found at the third stripping compared to the first two. It is hypothesized that these extra proteins after the third collection could be involved in some step of intracellular mechanism which is responsible for regulating of spermatozoa motility. However, protein identification revealed no significant distinction for any protein spot and protein sequences available in public databases. These results highlighted the need for a complete genome sequences for sturgeons.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22747924</PMID>
<DateCreated><Year>2013</Year>
<Month>01</Month>
<Day>15</Day>
</DateCreated>
<DateCompleted><Year>2013</Year>
<Month>07</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>01</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1439-0531</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>48</Volume>
<Issue>1</Issue>
<PubDate><Year>2013</Year>
<Month>Feb</Month>
</PubDate>
</JournalIssue>
<Title>Reproduction in domestic animals = Zuchthygiene</Title>
<ISOAbbreviation>Reprod. Domest. Anim.</ISOAbbreviation>
</Journal>
<ArticleTitle>Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus).</ArticleTitle>
<Pagination><MedlinePgn>156-9</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1439-0531.2012.02118.x</ELocationID>
<Abstract><AbstractText>Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed. Screening of these spots by mass spectrometric analysis showed positive results for spot 10. Direct comparison across public databases revealed sequence similarity with two hypothetical proteins, MCAG_00854 and IscW_ISCW011489. Differences in the seminal plasma protein fractions were found at the third stripping compared to the first two. It is hypothesized that these extra proteins after the third collection could be involved in some step of intracellular mechanism which is responsible for regulating of spermatozoa motility. However, protein identification revealed no significant distinction for any protein spot and protein sequences available in public databases. These results highlighted the need for a complete genome sequences for sturgeons.</AbstractText>
<CopyrightInformation>© 2012 Blackwell Verlag GmbH.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shaliutina</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hulak</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>P</ForeName>
<Initials>P</Initials>
</Author>
<Author ValidYN="Y"><LastName>Sulc</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Dzyuba</LastName>
<ForeName>B</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Linhart</LastName>
<ForeName>O</ForeName>
<Initials>O</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2012</Year>
<Month>07</Month>
<Day>02</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Reprod Domest Anim</MedlineTA>
<NlmUniqueID>9015668</NlmUniqueID>
<ISSNLinking>0936-6768</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029941">Fish Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029941" MajorTopicYN="N">Fish Proteins</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012661" MajorTopicYN="N">Semen</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year>
<Month>7</Month>
<Day>4</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2012</Year>
<Month>7</Month>
<Day>4</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2013</Year>
<Month>7</Month>
<Day>31</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">22747924</ArticleId>
<ArticleId IdType="doi">10.1111/j.1439-0531.2012.02118.x</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000276 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000276 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:22747924 |texte= Comparison of protein fractions in seminal plasma from multiple sperm collections in sterlet (Acipenser ruthenus). }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:22747924" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
![]() | This area was generated with Dilib version V0.6.27. | ![]() |