Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation.
Identifieur interne : 000165 ( PubMed/Corpus ); précédent : 000164; suivant : 000166Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation.
Auteurs : B. Dzyuba ; S. Boryshpolets ; J. Cosson ; V. Dzyuba ; P. Fedorov ; T. Saito ; M. Psenicka ; O. Linhart ; M. RodinaSource :
- Cryobiology [ 1090-2392 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- cytology : Spermatozoa, Testis, Wolffian Ducts.
- methods : Cryopreservation, Semen Preservation.
- physiology : Fishes.
- veterinary : Cryopreservation, Semen Preservation.
- Animals, Female, Fertilization in Vitro, Male, Sperm Motility.
Abstract
Sturgeon spermatozoa are immotile in the testis and acquire the potential for motility after contact with urine in Wolffian duct. The present study tested if in vitro incubation of testicular sperm in seminal fluid from Wolffian duct sperm leads to the acquisition of sperm fertilization ability. Sterlet sperm was taken from the testes, matured in vitro and cryopreserved. The fertility and motility of cryopreserved semen were tested. Matured testicular sperm showed freeze-thaw survival rates similar to Wolffian duct sperm, which is commonly used in sturgeon artificial propagation. Matured testicular sperm and Wolffian duct sperm post-thaw motility rate and curvilinear velocity were not significantly different, while duration of matured testicular sperm motility was significantly shorter than that of Wolffian duct sperm. Development rates of embryos obtained with post-thaw matured testicular sperm and Wolffian duct sperm were not significantly different. In vitro maturation of sterlet testicular sperm can potentially be useful in sperm cryobanking.
DOI: 10.1016/j.cryobiol.2014.07.008
PubMed: 25058859
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pubmed:25058859Le document en format XML
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Boryshpolets</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J" last="Cosson">J. Cosson</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Dzyuba, V" sort="Dzyuba, V" uniqKey="Dzyuba V" first="V" last="Dzyuba">V. Dzyuba</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic; Scientific-Research Institute of Biology, V.N. Karazin Kharkiv National University, Svobody Sq. 4, Kharkiv 61022, Ukraine.</nlm:affiliation></affiliation></author><author><name sortKey="Fedorov, P" sort="Fedorov, P" uniqKey="Fedorov P" first="P" last="Fedorov">P. Fedorov</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Saito, T" sort="Saito, T" uniqKey="Saito T" first="T" last="Saito">T. Saito</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M" last="Psenicka">M. Psenicka</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M" last="Rodina">M. Rodina</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25058859</idno><idno type="pmid">25058859</idno><idno type="doi">10.1016/j.cryobiol.2014.07.008</idno><idno type="wicri:Area/PubMed/Corpus">000165</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000165</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation.</title><author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic. Electronic address: bdzyuba@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S" last="Boryshpolets">S. Boryshpolets</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J" last="Cosson">J. Cosson</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Dzyuba, V" sort="Dzyuba, V" uniqKey="Dzyuba V" first="V" last="Dzyuba">V. Dzyuba</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic; Scientific-Research Institute of Biology, V.N. Karazin Kharkiv National University, Svobody Sq. 4, Kharkiv 61022, Ukraine.</nlm:affiliation></affiliation></author><author><name sortKey="Fedorov, P" sort="Fedorov, P" uniqKey="Fedorov P" first="P" last="Fedorov">P. Fedorov</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Saito, T" sort="Saito, T" uniqKey="Saito T" first="T" last="Saito">T. Saito</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M" last="Psenicka">M. Psenicka</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M" last="Rodina">M. Rodina</name><affiliation><nlm:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Cryobiology</title><idno type="eISSN">1090-2392</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cryopreservation (methods)</term><term>Cryopreservation (veterinary)</term><term>Female</term><term>Fertilization in Vitro</term><term>Fishes (physiology)</term><term>Male</term><term>Semen Preservation (methods)</term><term>Semen Preservation (veterinary)</term><term>Sperm Motility</term><term>Spermatozoa (cytology)</term><term>Testis (cytology)</term><term>Wolffian Ducts (cytology)</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spermatozoa</term><term>Testis</term><term>Wolffian Ducts</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term><term>Semen Preservation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Cryopreservation</term><term>Semen Preservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Fertilization in Vitro</term><term>Male</term><term>Sperm Motility</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sturgeon spermatozoa are immotile in the testis and acquire the potential for motility after contact with urine in Wolffian duct. The present study tested if in vitro incubation of testicular sperm in seminal fluid from Wolffian duct sperm leads to the acquisition of sperm fertilization ability. Sterlet sperm was taken from the testes, matured in vitro and cryopreserved. The fertility and motility of cryopreserved semen were tested. Matured testicular sperm showed freeze-thaw survival rates similar to Wolffian duct sperm, which is commonly used in sturgeon artificial propagation. Matured testicular sperm and Wolffian duct sperm post-thaw motility rate and curvilinear velocity were not significantly different, while duration of matured testicular sperm motility was significantly shorter than that of Wolffian duct sperm. Development rates of embryos obtained with post-thaw matured testicular sperm and Wolffian duct sperm were not significantly different. In vitro maturation of sterlet testicular sperm can potentially be useful in sperm cryobanking.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25058859</PMID><DateCreated><Year>2014</Year><Month>09</Month><Day>30</Day></DateCreated><DateCompleted><Year>2015</Year><Month>03</Month><Day>31</Day></DateCompleted><DateRevised><Year>2014</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2392</ISSN><JournalIssue CitedMedium="Internet"><Volume>69</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Cryobiology</Title><ISOAbbreviation>Cryobiology</ISOAbbreviation></Journal><ArticleTitle>Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation.</ArticleTitle><Pagination><MedlinePgn>339-41</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cryobiol.2014.07.008</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0011-2240(14)00196-5</ELocationID><Abstract><AbstractText>Sturgeon spermatozoa are immotile in the testis and acquire the potential for motility after contact with urine in Wolffian duct. The present study tested if in vitro incubation of testicular sperm in seminal fluid from Wolffian duct sperm leads to the acquisition of sperm fertilization ability. Sterlet sperm was taken from the testes, matured in vitro and cryopreserved. The fertility and motility of cryopreserved semen were tested. Matured testicular sperm showed freeze-thaw survival rates similar to Wolffian duct sperm, which is commonly used in sturgeon artificial propagation. Matured testicular sperm and Wolffian duct sperm post-thaw motility rate and curvilinear velocity were not significantly different, while duration of matured testicular sperm motility was significantly shorter than that of Wolffian duct sperm. Development rates of embryos obtained with post-thaw matured testicular sperm and Wolffian duct sperm were not significantly different. In vitro maturation of sterlet testicular sperm can potentially be useful in sperm cryobanking.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dzyuba</LastName><ForeName>B</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic. Electronic address: bdzyuba@frov.jcu.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boryshpolets</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cosson</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dzyuba</LastName><ForeName>V</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic; Scientific-Research Institute of Biology, V.N. Karazin Kharkiv National University, Svobody Sq. 4, Kharkiv 61022, Ukraine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fedorov</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Psenicka</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Linhart</LastName><ForeName>O</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodina</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>07</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Cryobiology</MedlineTA><NlmUniqueID>0006252</NlmUniqueID><ISSNLinking>0011-2240</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="N">Cryopreservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005307" MajorTopicYN="N">Fertilization in Vitro</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012662" MajorTopicYN="N">Semen Preservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013081" MajorTopicYN="N">Sperm Motility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013737" MajorTopicYN="N">Testis</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014928" MajorTopicYN="N">Wolffian Ducts</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Sperm maturation</Keyword><Keyword MajorTopicYN="N">Sterlet</Keyword><Keyword MajorTopicYN="N">Sturgeon conservation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>03</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate 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