Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis.
Identifieur interne : 000141 ( PubMed/Corpus ); précédent : 000140; suivant : 000142Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis.
Auteurs : Xiaoge Yang ; Huamei Yue ; Huan Ye ; Chuangju Li ; Qiwei WeiSource :
- Gene [ 1879-0038 ] ; 2015.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Biomarkers, Cloning, Molecular, Fish Proteins (analysis), Fish Proteins (genetics), Fish Proteins (metabolism), Gene Expression, Germ Cells (metabolism), In Situ Hybridization, Fluorescence, Molecular Sequence Data, Organ Specificity, Perciformes (embryology), Perciformes (genetics), Perciformes (metabolism), Phylogeny, RNA-Binding Proteins (analysis), RNA-Binding Proteins (genetics), RNA-Binding Proteins (metabolism), Sequence Alignment, Zebrafish.
- MESH :
- chemical , analysis : Fish Proteins, RNA-Binding Proteins.
- chemical , genetics : Fish Proteins, RNA-Binding Proteins.
- chemical , metabolism : Fish Proteins, RNA-Binding Proteins.
- chemical : Biomarkers.
- embryology : Perciformes.
- genetics : Perciformes.
- metabolism : Germ Cells, Perciformes.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Gene Expression, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Organ Specificity, Phylogeny, Sequence Alignment, Zebrafish.
Abstract
Dead end (dnd) encodes an RNA-binding protein that is essential for primordial germ cell (PGC) migration and gametogenesis in vertebrates. In this study, a Chinese sturgeon Acipenser sinensis dead end homologue, designated Asdnd, was identified and characterized. The full-length cDNA of Asdnd was 1630base pairs (bp) and encoded a peptide of 396 amino acid residues. Multiple sequence alignment showed that AsDnd shared six conserved regions of Dnd orthologs, including the RNA recognition motif. Phylogenetic analysis revealed that AsDnd was grouped with teleosts. By quantitative real-time PCR analysis, the Asdnd transcripts were found to originate from the maternal parent and be specifically expressed in gonads of immature Chinese sturgeons of both sexes. Fluorescent in situ hybridization indicated that Asdnd transcripts were restricted to germ cells. In the testis, Asdnd was abundant in spermatogonia and tended to gradually diminish in late spermatogenic stages, while strong signals were found in primary oocytes, as opposed to oogonia, in the ovary. Zebrafish PGCs were clearly visualized at 24h post-fertilization by co-injecting RFP-Asdnd 3' UTR and GFP-nos3 3' UTR mRNA, indicating that dnd 3' UTR has a conserved function among teleosts. Therefore, dnd could serve as a germ cell marker in Chinese sturgeon.
DOI: 10.1016/j.gene.2014.12.059
PubMed: 25550043
Links to Exploration step
pubmed:25550043Le document en format XML
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Electronic address: lcj@yfi.ac.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wei, Qiwei" sort="Wei, Qiwei" uniqKey="Wei Q" first="Qiwei" last="Wei">Qiwei Wei</name><affiliation><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China. 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Electronic address: lcj@yfi.ac.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Wei, Qiwei" sort="Wei, Qiwei" uniqKey="Wei Q" first="Qiwei" last="Wei">Qiwei Wei</name><affiliation><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China. Electronic address: weiqw@yfi.ac.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Gene</title><idno type="eISSN">1879-0038</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Biomarkers</term><term>Cloning, Molecular</term><term>Fish Proteins (analysis)</term><term>Fish Proteins (genetics)</term><term>Fish Proteins (metabolism)</term><term>Gene Expression</term><term>Germ Cells (metabolism)</term><term>In Situ Hybridization, Fluorescence</term><term>Molecular Sequence Data</term><term>Organ Specificity</term><term>Perciformes (embryology)</term><term>Perciformes (genetics)</term><term>Perciformes (metabolism)</term><term>Phylogeny</term><term>RNA-Binding Proteins (analysis)</term><term>RNA-Binding Proteins (genetics)</term><term>RNA-Binding Proteins (metabolism)</term><term>Sequence Alignment</term><term>Zebrafish</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Fish Proteins</term><term>RNA-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fish Proteins</term><term>RNA-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fish Proteins</term><term>RNA-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Perciformes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Perciformes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Germ Cells</term><term>Perciformes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Gene Expression</term><term>In Situ Hybridization, Fluorescence</term><term>Molecular Sequence Data</term><term>Organ Specificity</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Zebrafish</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dead end (dnd) encodes an RNA-binding protein that is essential for primordial germ cell (PGC) migration and gametogenesis in vertebrates. In this study, a Chinese sturgeon Acipenser sinensis dead end homologue, designated Asdnd, was identified and characterized. The full-length cDNA of Asdnd was 1630base pairs (bp) and encoded a peptide of 396 amino acid residues. Multiple sequence alignment showed that AsDnd shared six conserved regions of Dnd orthologs, including the RNA recognition motif. Phylogenetic analysis revealed that AsDnd was grouped with teleosts. By quantitative real-time PCR analysis, the Asdnd transcripts were found to originate from the maternal parent and be specifically expressed in gonads of immature Chinese sturgeons of both sexes. Fluorescent in situ hybridization indicated that Asdnd transcripts were restricted to germ cells. In the testis, Asdnd was abundant in spermatogonia and tended to gradually diminish in late spermatogenic stages, while strong signals were found in primary oocytes, as opposed to oogonia, in the ovary. Zebrafish PGCs were clearly visualized at 24h post-fertilization by co-injecting RFP-Asdnd 3' UTR and GFP-nos3 3' UTR mRNA, indicating that dnd 3' UTR has a conserved function among teleosts. Therefore, dnd could serve as a germ cell marker in Chinese sturgeon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25550043</PMID><DateCreated><Year>2015</Year><Month>01</Month><Day>24</Day></DateCreated><DateCompleted><Year>2015</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0038</ISSN><JournalIssue CitedMedium="Internet"><Volume>558</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Mar</Month><Day>01</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis.</ArticleTitle><Pagination><MedlinePgn>118-25</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.gene.2014.12.059</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0378-1119(14)01485-1</ELocationID><Abstract><AbstractText>Dead end (dnd) encodes an RNA-binding protein that is essential for primordial germ cell (PGC) migration and gametogenesis in vertebrates. In this study, a Chinese sturgeon Acipenser sinensis dead end homologue, designated Asdnd, was identified and characterized. The full-length cDNA of Asdnd was 1630base pairs (bp) and encoded a peptide of 396 amino acid residues. Multiple sequence alignment showed that AsDnd shared six conserved regions of Dnd orthologs, including the RNA recognition motif. Phylogenetic analysis revealed that AsDnd was grouped with teleosts. By quantitative real-time PCR analysis, the Asdnd transcripts were found to originate from the maternal parent and be specifically expressed in gonads of immature Chinese sturgeons of both sexes. Fluorescent in situ hybridization indicated that Asdnd transcripts were restricted to germ cells. In the testis, Asdnd was abundant in spermatogonia and tended to gradually diminish in late spermatogenic stages, while strong signals were found in primary oocytes, as opposed to oogonia, in the ovary. Zebrafish PGCs were clearly visualized at 24h post-fertilization by co-injecting RFP-Asdnd 3' UTR and GFP-nos3 3' UTR mRNA, indicating that dnd 3' UTR has a conserved function among teleosts. Therefore, dnd could serve as a germ cell marker in Chinese sturgeon.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiaoge</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yue</LastName><ForeName>Huamei</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Huan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chuangju</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China. Electronic address: lcj@yfi.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Qiwei</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China. 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