Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis.
Identifieur interne : 000087 ( PubMed/Corpus ); précédent : 000086; suivant : 000088Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis.
Auteurs : X Q Leng ; H J Du ; C J Li ; H. CaoSource :
- Journal of fish biology [ 1095-8649 ] ; 2016.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cloning, Molecular, DNA, Complementary (genetics), Female, Fishes (genetics), Fishes (growth & development), Fishes (metabolism), Germ Cells (metabolism), Male, Ovary (metabolism), RNA, Messenger (metabolism), Sex Differentiation, Spermatogenesis, Testis (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism).
- MESH :
- chemical , genetics : DNA, Complementary, Transcription Factors.
- genetics : Fishes.
- growth & development : Fishes.
- metabolism : Fishes, Germ Cells, Ovary, RNA, Messenger, Testis, Transcription Factors.
- Amino Acid Sequence, Animals, Cloning, Molecular, Female, Male, Sex Differentiation, Spermatogenesis.
Abstract
In this study, the cDNA of dmrt1 gene from the Chinese sturgeon Acipenser sinensis was isolated and its expression pattern was characterized in different tissues of immature A. sinensis. By real-time quantitative PCR (qrtPCR) analysis, the A. sinensis dmrt1 mRNA was detected mainly in gonad and with a higher level in the testis than the ovary, especially in 3 and 4 year-old samples. This indicated that the dmrt1 expression exhibited gradual testis specificity with development. The subcellular localization analysis indicated that the Dmrt1 protein exists only in germ cells and not in somatic cells. These results suggest that A. sinensis dmrt1 might be a highly specific sex differentiation gene for testis development and spermatogenesis.
DOI: 10.1111/jfb.12852
PubMed: 26706998
Links to Exploration step
pubmed:26706998Le document en format XML
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Cao</name><affiliation><nlm:affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26706998</idno><idno type="pmid">26706998</idno><idno type="doi">10.1111/jfb.12852</idno><idno type="wicri:Area/PubMed/Corpus">000087</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000087</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis.</title><author><name sortKey="Leng, X Q" sort="Leng, X Q" uniqKey="Leng X" first="X Q" last="Leng">X Q Leng</name><affiliation><nlm:affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.</nlm:affiliation></affiliation></author><author><name sortKey="Du, H J" sort="Du, H J" uniqKey="Du H" first="H J" last="Du">H J Du</name><affiliation><nlm:affiliation>Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, C J" sort="Li, C J" uniqKey="Li C" first="C J" last="Li">C J Li</name><affiliation><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Agriculture Ministry of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, H" sort="Cao, H" uniqKey="Cao H" first="H" last="Cao">H. Cao</name><affiliation><nlm:affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of fish biology</title><idno type="eISSN">1095-8649</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>DNA, Complementary (genetics)</term><term>Female</term><term>Fishes (genetics)</term><term>Fishes (growth & development)</term><term>Fishes (metabolism)</term><term>Germ Cells (metabolism)</term><term>Male</term><term>Ovary (metabolism)</term><term>RNA, Messenger (metabolism)</term><term>Sex Differentiation</term><term>Spermatogenesis</term><term>Testis (metabolism)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Germ Cells</term><term>Ovary</term><term>RNA, Messenger</term><term>Testis</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>Female</term><term>Male</term><term>Sex Differentiation</term><term>Spermatogenesis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, the cDNA of dmrt1 gene from the Chinese sturgeon Acipenser sinensis was isolated and its expression pattern was characterized in different tissues of immature A. sinensis. By real-time quantitative PCR (qrtPCR) analysis, the A. sinensis dmrt1 mRNA was detected mainly in gonad and with a higher level in the testis than the ovary, especially in 3 and 4 year-old samples. This indicated that the dmrt1 expression exhibited gradual testis specificity with development. The subcellular localization analysis indicated that the Dmrt1 protein exists only in germ cells and not in somatic cells. These results suggest that A. sinensis dmrt1 might be a highly specific sex differentiation gene for testis development and spermatogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26706998</PMID><DateCreated><Year>2016</Year><Month>02</Month><Day>19</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>06</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-8649</ISSN><JournalIssue CitedMedium="Internet"><Volume>88</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of fish biology</Title><ISOAbbreviation>J. Fish Biol.</ISOAbbreviation></Journal><ArticleTitle>Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis.</ArticleTitle><Pagination><MedlinePgn>567-79</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/jfb.12852</ELocationID><Abstract><AbstractText>In this study, the cDNA of dmrt1 gene from the Chinese sturgeon Acipenser sinensis was isolated and its expression pattern was characterized in different tissues of immature A. sinensis. By real-time quantitative PCR (qrtPCR) analysis, the A. sinensis dmrt1 mRNA was detected mainly in gonad and with a higher level in the testis than the ovary, especially in 3 and 4 year-old samples. This indicated that the dmrt1 expression exhibited gradual testis specificity with development. The subcellular localization analysis indicated that the Dmrt1 protein exists only in germ cells and not in somatic cells. These results suggest that A. sinensis dmrt1 might be a highly specific sex differentiation gene for testis development and spermatogenesis.</AbstractText><CopyrightInformation>© 2015 The Fisheries Society of the British Isles.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Leng</LastName><ForeName>X Q</ForeName><Initials>XQ</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Agriculture Ministry of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>H J</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>C J</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Agriculture Ministry of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>12</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Fish Biol</MedlineTA><NlmUniqueID>0214055</NlmUniqueID><ISSNLinking>0022-1112</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C110547">DMRT1 protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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Differentiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013091" MajorTopicYN="N">Spermatogenesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013737" MajorTopicYN="N">Testis</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">dsx- and mab-3-related transcription factor 1</Keyword><Keyword MajorTopicYN="N">sex differentiation</Keyword><Keyword MajorTopicYN="N">subcellular localization</Keyword><Keyword MajorTopicYN="N">testis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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