Effect of freezing rate for cryopreservation of Persian sturgeon (Acipenser persicus) spermatozoa.
Identifieur interne : 000082 ( PubMed/Corpus ); précédent : 000081; suivant : 000083Effect of freezing rate for cryopreservation of Persian sturgeon (Acipenser persicus) spermatozoa.
Auteurs : Mohammad Sadegh Aramli ; Karim Golshahi ; Rajab Mohammad Nazari ; Ebrahim Sotoudeh ; Salim Aramli ; Ensieh HabibiSource :
- Theriogenology [ 1879-3231 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- methods : Semen Preservation.
- physiology : Fishes, Spermatozoa.
- veterinary : Semen Preservation.
- Animals, Cryopreservation, Male, Time Factors.
Abstract
This study examined the effect of freezing rate (-10 °C, -15 °C, -20 °C, -30 °C, and -40 °C/min) on motility parameters, rates of fertilization and hatching, ATP content, and indices of oxidative stress including thiobarbituric acid reactive substances and carbonyl derivatives of proteins in Persian sturgeon (Acipenser persicus) sperm. After sampling, sperm was diluted in an extender composed of 23.4-mM sucrose, 0.25-mM KCl, and 30-mM Tris-HCl, pH 8.0, containing 10% methanol and subsequently frozen in a programmable freezer. For postthaw sperm that were frozen at a rate of -40 °C/min, sperm motile duration (134 ± 27.01 seconds), sperm motile percent (60 ± 4.1%), fertilizability (72 ± 8.36% for fertilization rate and 65 ± 7.58% for hatching rate), and ATP content (4.8 ± 0.57 nmol/10(8) sperm) were significantly higher than for sperm frozen at any of the four slower rates (P < 0.05). Moreover, sperm cryopreserved using the fastest freezing rate had significantly lower levels of thiobarbituric acid reactive substances (0.5 ± 0.05 nmol/10(8) sperm) and carbonyl derivatives of proteins (41.3 ± 4.9 nmol/10(8) sperm) than sperm cryopreserved using all other freezing rates (P < 0.05). In addition, there is a significant difference (P < 0.05) between fresh sperm and the recovery of cryopreserved Persian sturgeon sperm using programmable freezing with -40 °C/min being the optimal freezing rate among those tested.
DOI: 10.1016/j.theriogenology.2015.10.018
PubMed: 26549121
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Electronic address: msaramli@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Golshahi, Karim" sort="Golshahi, Karim" uniqKey="Golshahi K" first="Karim" last="Golshahi">Karim Golshahi</name><affiliation><nlm:affiliation>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Nazari, Rajab Mohammad" sort="Nazari, Rajab Mohammad" uniqKey="Nazari R" first="Rajab Mohammad" last="Nazari">Rajab Mohammad Nazari</name><affiliation><nlm:affiliation>Rajaei Sturgeon Hatchery Center, Sari, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Sotoudeh, Ebrahim" sort="Sotoudeh, Ebrahim" uniqKey="Sotoudeh E" first="Ebrahim" last="Sotoudeh">Ebrahim Sotoudeh</name><affiliation><nlm:affiliation>Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Aramli, Salim" sort="Aramli, Salim" uniqKey="Aramli S" first="Salim" last="Aramli">Salim Aramli</name><affiliation><nlm:affiliation>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Habibi, Ensieh" sort="Habibi, Ensieh" uniqKey="Habibi E" first="Ensieh" last="Habibi">Ensieh Habibi</name><affiliation><nlm:affiliation>Biotechnology Laboratory, Faculty of Agriculture and Natural Resources, Tehran University, Karaj, Iran.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26549121</idno><idno type="pmid">26549121</idno><idno type="doi">10.1016/j.theriogenology.2015.10.018</idno><idno type="wicri:Area/PubMed/Corpus">000082</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000082</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effect of freezing rate for cryopreservation of Persian sturgeon (Acipenser persicus) spermatozoa.</title><author><name sortKey="Aramli, Mohammad Sadegh" sort="Aramli, Mohammad Sadegh" uniqKey="Aramli M" first="Mohammad Sadegh" last="Aramli">Mohammad Sadegh Aramli</name><affiliation><nlm:affiliation>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran. Electronic address: msaramli@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Golshahi, Karim" sort="Golshahi, Karim" uniqKey="Golshahi K" first="Karim" last="Golshahi">Karim Golshahi</name><affiliation><nlm:affiliation>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Nazari, Rajab Mohammad" sort="Nazari, Rajab Mohammad" uniqKey="Nazari R" first="Rajab Mohammad" last="Nazari">Rajab Mohammad Nazari</name><affiliation><nlm:affiliation>Rajaei Sturgeon Hatchery Center, Sari, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Sotoudeh, Ebrahim" sort="Sotoudeh, Ebrahim" uniqKey="Sotoudeh E" first="Ebrahim" last="Sotoudeh">Ebrahim Sotoudeh</name><affiliation><nlm:affiliation>Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Aramli, Salim" sort="Aramli, Salim" uniqKey="Aramli S" first="Salim" last="Aramli">Salim Aramli</name><affiliation><nlm:affiliation>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Habibi, Ensieh" sort="Habibi, Ensieh" uniqKey="Habibi E" first="Ensieh" last="Habibi">Ensieh Habibi</name><affiliation><nlm:affiliation>Biotechnology Laboratory, Faculty of Agriculture and Natural Resources, Tehran University, Karaj, Iran.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Theriogenology</title><idno type="eISSN">1879-3231</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cryopreservation</term><term>Fishes (physiology)</term><term>Male</term><term>Semen Preservation (methods)</term><term>Semen Preservation (veterinary)</term><term>Spermatozoa (physiology)</term><term>Time Factors</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Semen Preservation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Semen Preservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cryopreservation</term><term>Male</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study examined the effect of freezing rate (-10 °C, -15 °C, -20 °C, -30 °C, and -40 °C/min) on motility parameters, rates of fertilization and hatching, ATP content, and indices of oxidative stress including thiobarbituric acid reactive substances and carbonyl derivatives of proteins in Persian sturgeon (Acipenser persicus) sperm. After sampling, sperm was diluted in an extender composed of 23.4-mM sucrose, 0.25-mM KCl, and 30-mM Tris-HCl, pH 8.0, containing 10% methanol and subsequently frozen in a programmable freezer. For postthaw sperm that were frozen at a rate of -40 °C/min, sperm motile duration (134 ± 27.01 seconds), sperm motile percent (60 ± 4.1%), fertilizability (72 ± 8.36% for fertilization rate and 65 ± 7.58% for hatching rate), and ATP content (4.8 ± 0.57 nmol/10(8) sperm) were significantly higher than for sperm frozen at any of the four slower rates (P < 0.05). Moreover, sperm cryopreserved using the fastest freezing rate had significantly lower levels of thiobarbituric acid reactive substances (0.5 ± 0.05 nmol/10(8) sperm) and carbonyl derivatives of proteins (41.3 ± 4.9 nmol/10(8) sperm) than sperm cryopreserved using all other freezing rates (P < 0.05). In addition, there is a significant difference (P < 0.05) between fresh sperm and the recovery of cryopreserved Persian sturgeon sperm using programmable freezing with -40 °C/min being the optimal freezing rate among those tested.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26549121</PMID><DateCreated><Year>2016</Year><Month>02</Month><Day>01</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>31</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-3231</ISSN><JournalIssue CitedMedium="Internet"><Volume>85</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>Mar</Month><Day>01</Day></PubDate></JournalIssue><Title>Theriogenology</Title><ISOAbbreviation>Theriogenology</ISOAbbreviation></Journal><ArticleTitle>Effect of freezing rate for cryopreservation of Persian sturgeon (Acipenser persicus) spermatozoa.</ArticleTitle><Pagination><MedlinePgn>734-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.theriogenology.2015.10.018</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0093-691X(15)00560-9</ELocationID><Abstract><AbstractText>This study examined the effect of freezing rate (-10 °C, -15 °C, -20 °C, -30 °C, and -40 °C/min) on motility parameters, rates of fertilization and hatching, ATP content, and indices of oxidative stress including thiobarbituric acid reactive substances and carbonyl derivatives of proteins in Persian sturgeon (Acipenser persicus) sperm. After sampling, sperm was diluted in an extender composed of 23.4-mM sucrose, 0.25-mM KCl, and 30-mM Tris-HCl, pH 8.0, containing 10% methanol and subsequently frozen in a programmable freezer. For postthaw sperm that were frozen at a rate of -40 °C/min, sperm motile duration (134 ± 27.01 seconds), sperm motile percent (60 ± 4.1%), fertilizability (72 ± 8.36% for fertilization rate and 65 ± 7.58% for hatching rate), and ATP content (4.8 ± 0.57 nmol/10(8) sperm) were significantly higher than for sperm frozen at any of the four slower rates (P < 0.05). Moreover, sperm cryopreserved using the fastest freezing rate had significantly lower levels of thiobarbituric acid reactive substances (0.5 ± 0.05 nmol/10(8) sperm) and carbonyl derivatives of proteins (41.3 ± 4.9 nmol/10(8) sperm) than sperm cryopreserved using all other freezing rates (P < 0.05). In addition, there is a significant difference (P < 0.05) between fresh sperm and the recovery of cryopreserved Persian sturgeon sperm using programmable freezing with -40 °C/min being the optimal freezing rate among those tested.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aramli</LastName><ForeName>Mohammad Sadegh</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran. Electronic address: msaramli@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Golshahi</LastName><ForeName>Karim</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nazari</LastName><ForeName>Rajab Mohammad</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Rajaei Sturgeon Hatchery Center, Sari, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sotoudeh</LastName><ForeName>Ebrahim</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aramli</LastName><ForeName>Salim</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Habibi</LastName><ForeName>Ensieh</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Biotechnology Laboratory, Faculty of Agriculture and Natural Resources, Tehran University, Karaj, Iran.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Theriogenology</MedlineTA><NlmUniqueID>0421510</NlmUniqueID><ISSNLinking>0093-691X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="Y">Cryopreservation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012662" MajorTopicYN="N">Semen Preservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ATP</Keyword><Keyword MajorTopicYN="N">Cryopreservation</Keyword><Keyword MajorTopicYN="N">Fertility</Keyword><Keyword MajorTopicYN="N">Oxidative stress</Keyword><Keyword MajorTopicYN="N">Persian sturgeon</Keyword><Keyword MajorTopicYN="N">Sperm motility</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>10</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>11</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26549121</ArticleId><ArticleId IdType="pii">S0093-691X(15)00560-9</ArticleId><ArticleId IdType="doi">10.1016/j.theriogenology.2015.10.018</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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