Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.
Identifieur interne : 000015 ( PubMed/Corpus ); précédent : 000014; suivant : 000016Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.
Auteurs : Shubo Jin ; Dajiang Sun ; Qingkai Xi ; Xiaoli Dong ; Dan Song ; Hongtuo Fu ; Ying ZhangSource :
- Gene [ 1879-0038 ] ; 2016.
English descriptors
- KwdEn :
- Animals, Female, Fish Proteins (biosynthesis), Fish Proteins (genetics), Fishes (genetics), Fishes (metabolism), Gene Expression Regulation (drug effects), Gonads (metabolism), Hypothalamo-Hypophyseal System (metabolism), Kisspeptins (pharmacology), Pregnancy, Reproduction (drug effects), Sex Differentiation (drug effects), Transcriptome (drug effects).
- MESH :
- chemical , biosynthesis : Fish Proteins.
- chemical , genetics : Fish Proteins.
- drug effects : Gene Expression Regulation, Reproduction, Sex Differentiation, Transcriptome.
- genetics : Fishes.
- metabolism : Fishes, Gonads, Hypothalamo-Hypophyseal System.
- chemical , pharmacology : Kisspeptins.
- Animals, Female, Pregnancy.
Abstract
Kisspeptin plays an important role in the reproduction and onset of puberty in vertebrates through stimulation of gonadotropin-releasing hormone (GnRH). However, the mechanisms whereby kisspeptin-related genes regulate sexual differentiation in teleosts are poorly understood. We aimed to study the relationship between the hypothalamus-pituitary-gonad (HPG) axis and sexual differentiation in relation to kisspeptin in the sturgeon Acipenser schrenckii. We performed comparative transcriptomic analysis of the brains of sturgeons treated with KISS1-10 during the gonadal sex-differentiation-sensitive period (170-210days post-hatching (dph)) using an Illumina sequencing platform. We also analyzed mRNA expression levels of genes in the HPG axis using real-time quantitative polymerase chain reaction, and measured estradiol-17β (E2) and testosterone (T) levels in the brain and gonads using radioimmunological methods. A total of 75,960 and 74,907 unigenes were produced from Kisspeptin-treated and physiological saline-treated fish, respectively, among which 47,891 genes were matched to the non-redundant nr database. Potential genes and their functions were identified by GO (32,435), KEGG (37,619), and COG analyses (18,502). A total of 3169 unigenes were differentially expressed between transcriptomes in KISS1-10- and saline-injected fish, including 300 up-regulated and 2869 down-regulated unigenes. Gene expression levels of KISS1, G protein-coupled receptor-54, GnRH, androgen receptor, estrogen receptor, and Cyp19a in the brain and gonad were significantly affected by KISS1-10 treatment. KISS1-10 injection also significantly increased brain levels of E2 and T, compared with controls. These results support important roles for KISS1 in the regulation of the HPG axis, and in sex differentiation and reproduction in the Amur sturgeon.
DOI: 10.1016/j.gene.2016.09.026
PubMed: 27688073
Links to Exploration step
pubmed:27688073Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.</title><author><name sortKey="Jin, Shubo" sort="Jin, Shubo" uniqKey="Jin S" first="Shubo" last="Jin">Shubo Jin</name><affiliation><nlm:affiliation>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Dajiang" sort="Sun, Dajiang" uniqKey="Sun D" first="Dajiang" last="Sun">Dajiang Sun</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Xi, Qingkai" sort="Xi, Qingkai" uniqKey="Xi Q" first="Qingkai" last="Xi">Qingkai Xi</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Dong, Xiaoli" sort="Dong, Xiaoli" uniqKey="Dong X" first="Xiaoli" last="Dong">Xiaoli Dong</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Dan" sort="Song, Dan" uniqKey="Song D" first="Dan" last="Song">Dan Song</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Fu, Hongtuo" sort="Fu, Hongtuo" uniqKey="Fu H" first="Hongtuo" last="Fu">Hongtuo Fu</name><affiliation><nlm:affiliation>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.. Electronic address: juletzhang@hotmail.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27688073</idno><idno type="pmid">27688073</idno><idno type="doi">10.1016/j.gene.2016.09.026</idno><idno type="wicri:Area/PubMed/Corpus">000015</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000015</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.</title><author><name sortKey="Jin, Shubo" sort="Jin, Shubo" uniqKey="Jin S" first="Shubo" last="Jin">Shubo Jin</name><affiliation><nlm:affiliation>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Dajiang" sort="Sun, Dajiang" uniqKey="Sun D" first="Dajiang" last="Sun">Dajiang Sun</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Xi, Qingkai" sort="Xi, Qingkai" uniqKey="Xi Q" first="Qingkai" last="Xi">Qingkai Xi</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Dong, Xiaoli" sort="Dong, Xiaoli" uniqKey="Dong X" first="Xiaoli" last="Dong">Xiaoli Dong</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Dan" sort="Song, Dan" uniqKey="Song D" first="Dan" last="Song">Dan Song</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Fu, Hongtuo" sort="Fu, Hongtuo" uniqKey="Fu H" first="Hongtuo" last="Fu">Hongtuo Fu</name><affiliation><nlm:affiliation>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name><affiliation><nlm:affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.. Electronic address: juletzhang@hotmail.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Gene</title><idno type="eISSN">1879-0038</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Female</term><term>Fish Proteins (biosynthesis)</term><term>Fish Proteins (genetics)</term><term>Fishes (genetics)</term><term>Fishes (metabolism)</term><term>Gene Expression Regulation (drug effects)</term><term>Gonads (metabolism)</term><term>Hypothalamo-Hypophyseal System (metabolism)</term><term>Kisspeptins (pharmacology)</term><term>Pregnancy</term><term>Reproduction (drug effects)</term><term>Sex Differentiation (drug effects)</term><term>Transcriptome (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Fish Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fish Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation</term><term>Reproduction</term><term>Sex Differentiation</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Gonads</term><term>Hypothalamo-Hypophyseal System</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Kisspeptins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Pregnancy</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Kisspeptin plays an important role in the reproduction and onset of puberty in vertebrates through stimulation of gonadotropin-releasing hormone (GnRH). However, the mechanisms whereby kisspeptin-related genes regulate sexual differentiation in teleosts are poorly understood. We aimed to study the relationship between the hypothalamus-pituitary-gonad (HPG) axis and sexual differentiation in relation to kisspeptin in the sturgeon Acipenser schrenckii. We performed comparative transcriptomic analysis of the brains of sturgeons treated with KISS1-10 during the gonadal sex-differentiation-sensitive period (170-210days post-hatching (dph)) using an Illumina sequencing platform. We also analyzed mRNA expression levels of genes in the HPG axis using real-time quantitative polymerase chain reaction, and measured estradiol-17β (E2) and testosterone (T) levels in the brain and gonads using radioimmunological methods. A total of 75,960 and 74,907 unigenes were produced from Kisspeptin-treated and physiological saline-treated fish, respectively, among which 47,891 genes were matched to the non-redundant nr database. Potential genes and their functions were identified by GO (32,435), KEGG (37,619), and COG analyses (18,502). A total of 3169 unigenes were differentially expressed between transcriptomes in KISS1-10- and saline-injected fish, including 300 up-regulated and 2869 down-regulated unigenes. Gene expression levels of KISS1, G protein-coupled receptor-54, GnRH, androgen receptor, estrogen receptor, and Cyp19a in the brain and gonad were significantly affected by KISS1-10 treatment. KISS1-10 injection also significantly increased brain levels of E2 and T, compared with controls. These results support important roles for KISS1 in the regulation of the HPG axis, and in sex differentiation and reproduction in the Amur sturgeon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27688073</PMID><DateCreated><Year>2016</Year><Month>09</Month><Day>30</Day></DateCreated><DateCompleted><Year>2017</Year><Month>01</Month><Day>25</Day></DateCompleted><DateRevised><Year>2017</Year><Month>01</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0038</ISSN><JournalIssue CitedMedium="Internet"><Volume>595</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Dec</Month><Day>20</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.</ArticleTitle><Pagination><MedlinePgn>53-61</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0378-1119(16)30753-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.gene.2016.09.026</ELocationID><Abstract><AbstractText NlmCategory="UNASSIGNED">Kisspeptin plays an important role in the reproduction and onset of puberty in vertebrates through stimulation of gonadotropin-releasing hormone (GnRH). However, the mechanisms whereby kisspeptin-related genes regulate sexual differentiation in teleosts are poorly understood. We aimed to study the relationship between the hypothalamus-pituitary-gonad (HPG) axis and sexual differentiation in relation to kisspeptin in the sturgeon Acipenser schrenckii. We performed comparative transcriptomic analysis of the brains of sturgeons treated with KISS1-10 during the gonadal sex-differentiation-sensitive period (170-210days post-hatching (dph)) using an Illumina sequencing platform. We also analyzed mRNA expression levels of genes in the HPG axis using real-time quantitative polymerase chain reaction, and measured estradiol-17β (E2) and testosterone (T) levels in the brain and gonads using radioimmunological methods. A total of 75,960 and 74,907 unigenes were produced from Kisspeptin-treated and physiological saline-treated fish, respectively, among which 47,891 genes were matched to the non-redundant nr database. Potential genes and their functions were identified by GO (32,435), KEGG (37,619), and COG analyses (18,502). A total of 3169 unigenes were differentially expressed between transcriptomes in KISS1-10- and saline-injected fish, including 300 up-regulated and 2869 down-regulated unigenes. Gene expression levels of KISS1, G protein-coupled receptor-54, GnRH, androgen receptor, estrogen receptor, and Cyp19a in the brain and gonad were significantly affected by KISS1-10 treatment. KISS1-10 injection also significantly increased brain levels of E2 and T, compared with controls. These results support important roles for KISS1 in the regulation of the HPG axis, and in sex differentiation and reproduction in the Amur sturgeon.</AbstractText><CopyrightInformation>Copyright © 2016. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Shubo</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Dajiang</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xi</LastName><ForeName>Qingkai</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Xiaoli</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Dan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Hongtuo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, PR China.. Electronic address: juletzhang@hotmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>09</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Gene</MedlineTA><NlmUniqueID>7706761</NlmUniqueID><ISSNLinking>0378-1119</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029941">Fish Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D059648">Kisspeptins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029941" MajorTopicYN="N">Fish Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006066" MajorTopicYN="N">Gonads</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007030" MajorTopicYN="N">Hypothalamo-Hypophyseal System</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059648" MajorTopicYN="N">Kisspeptins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011247" MajorTopicYN="N">Pregnancy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012098" MajorTopicYN="N">Reproduction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012733" MajorTopicYN="N">Sex Differentiation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Acipenser schrenckii</Keyword><Keyword MajorTopicYN="N">Gene</Keyword><Keyword MajorTopicYN="N">Hypothalamus–pituitary–gonad axis</Keyword><Keyword MajorTopicYN="N">Kisspeptin</Keyword><Keyword MajorTopicYN="N">Real-time quantitative PCR</Keyword><Keyword MajorTopicYN="N">Transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>09</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27688073</ArticleId><ArticleId IdType="pii">S0378-1119(16)30753-3</ArticleId><ArticleId IdType="doi">10.1016/j.gene.2016.09.026</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000015 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000015 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:27688073
|texte= Identification of genes in the hypothalamus-pituitary-gonad axis in the brain of Amur sturgeons (Acipenser schrenckii) by comparative transcriptome analysis in relation to kisspeptin treatment.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:27688073" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |