Ig light chain gene in the Siberian sturgeon (Acipenser baeri).
Identifieur interne : 000636 ( PubMed/Checkpoint ); précédent : 000635; suivant : 000637Ig light chain gene in the Siberian sturgeon (Acipenser baeri).
Auteurs : M. Lundqvist [Suède] ; E. Bengtén ; S. Strömberg ; L. PilströmSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1996.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibody Diversity (genetics), Base Sequence, Cloning, Molecular, DNA, Complementary (isolation & purification), Evolution, Molecular, Fishes (genetics), Fishes (immunology), Genes, Immunoglobulin (genetics), Genes, Immunoglobulin (immunology), Molecular Sequence Data, Transcription, Genetic (immunology).
- MESH :
- chemical , isolation & purification : DNA, Complementary.
- genetics : Antibody Diversity, Fishes, Genes, Immunoglobulin.
- immunology : Fishes, Genes, Immunoglobulin, Transcription, Genetic.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Evolution, Molecular, Molecular Sequence Data.
Abstract
Elasmobranch and teleost fish have their Ig light (L) chain loci organized in multiple clusters (VL-JL-CL). The VL segments of teleosts are in opposite transcriptional orientation to the CL genes, suggesting that in teleosts and elasmobranchs there may have been separate evolutionary events leading to this organization. To address this problem, the IgL locus from the Siberian sturgeon (Acipenser baeri) (representative of a branch between elasmobranchs and teleosts) was investigated. Sequence analysis of cDNA clones shows that sturgeon VL genes are most similar to those of teleosts, but that sturgeon CL genes are more similar to those of the sharks. Southern blot analyses of sturgeon erythrocyte DNA with VL- and CL-specific probes showed that there are more than 20 VL segments in both the tetraploid Siberian sturgeon and the diploid sterlet (Acipenser ruthenus), but only a few CL segments in the genome of the Siberian sturgeon and up to four CL segments in that of the sterlet. Screening of an unamplified genomic library gave more than 300 VL-positive and four CL-positive clones. None of these contained inserts positive for both probes. PCR analysis of a genomic CL clone using IC and CL-specific primers suggested that upstream of the CL segment there are at least seven JL segments. it is concluded that sturgeons have a kappa-like organization of their IgL locus and that the clustered organization of IgL loci in bony fish and sharks arose from two distinct evolutionary events.
PubMed: 8757324
Affiliations:
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Bengtén</name></author><author><name sortKey="Stromberg, S" sort="Stromberg, S" uniqKey="Stromberg S" first="S" last="Strömberg">S. Strömberg</name></author><author><name sortKey="Pilstrom, L" sort="Pilstrom, L" uniqKey="Pilstrom L" first="L" last="Pilström">L. Pilström</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8757324</idno><idno type="pmid">8757324</idno><idno type="wicri:Area/PubMed/Corpus">000682</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000682</idno><idno type="wicri:Area/PubMed/Curation">000682</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000682</idno><idno type="wicri:Area/PubMed/Checkpoint">000682</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000682</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ig light chain gene in the Siberian sturgeon (Acipenser baeri).</title><author><name sortKey="Lundqvist, M" sort="Lundqvist, M" uniqKey="Lundqvist M" first="M" last="Lundqvist">M. Lundqvist</name><affiliation wicri:level="4"><nlm:affiliation>Department of Medical Immunology and Microbiology, BMC, Uppsala University, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Medical Immunology and Microbiology, BMC, Uppsala University</wicri:regionArea><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName><orgName type="university">Université d'Uppsala</orgName></affiliation></author><author><name sortKey="Bengten, E" sort="Bengten, E" uniqKey="Bengten E" first="E" last="Bengtén">E. Bengtén</name></author><author><name sortKey="Stromberg, S" sort="Stromberg, S" uniqKey="Stromberg S" first="S" last="Strömberg">S. Strömberg</name></author><author><name sortKey="Pilstrom, L" sort="Pilstrom, L" uniqKey="Pilstrom L" first="L" last="Pilström">L. Pilström</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibody Diversity (genetics)</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>DNA, Complementary (isolation & purification)</term><term>Evolution, Molecular</term><term>Fishes (genetics)</term><term>Fishes (immunology)</term><term>Genes, Immunoglobulin (genetics)</term><term>Genes, Immunoglobulin (immunology)</term><term>Molecular Sequence Data</term><term>Transcription, Genetic (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Antibody Diversity</term><term>Fishes</term><term>Genes, Immunoglobulin</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Fishes</term><term>Genes, Immunoglobulin</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Evolution, Molecular</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Elasmobranch and teleost fish have their Ig light (L) chain loci organized in multiple clusters (VL-JL-CL). The VL segments of teleosts are in opposite transcriptional orientation to the CL genes, suggesting that in teleosts and elasmobranchs there may have been separate evolutionary events leading to this organization. To address this problem, the IgL locus from the Siberian sturgeon (Acipenser baeri) (representative of a branch between elasmobranchs and teleosts) was investigated. Sequence analysis of cDNA clones shows that sturgeon VL genes are most similar to those of teleosts, but that sturgeon CL genes are more similar to those of the sharks. Southern blot analyses of sturgeon erythrocyte DNA with VL- and CL-specific probes showed that there are more than 20 VL segments in both the tetraploid Siberian sturgeon and the diploid sterlet (Acipenser ruthenus), but only a few CL segments in the genome of the Siberian sturgeon and up to four CL segments in that of the sterlet. Screening of an unamplified genomic library gave more than 300 VL-positive and four CL-positive clones. None of these contained inserts positive for both probes. PCR analysis of a genomic CL clone using IC and CL-specific primers suggested that upstream of the CL segment there are at least seven JL segments. it is concluded that sturgeons have a kappa-like organization of their IgL locus and that the clustered organization of IgL loci in bony fish and sharks arose from two distinct evolutionary events.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8757324</PMID><DateCreated><Year>1996</Year><Month>11</Month><Day>22</Day></DateCreated><DateCompleted><Year>1996</Year><Month>11</Month><Day>22</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1767</ISSN><JournalIssue CitedMedium="Print"><Volume>157</Volume><Issue>5</Issue><PubDate><Year>1996</Year><Month>Sep</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of immunology (Baltimore, Md. : 1950)</Title><ISOAbbreviation>J. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Ig light chain gene in the Siberian sturgeon (Acipenser baeri).</ArticleTitle><Pagination><MedlinePgn>2031-8</MedlinePgn></Pagination><Abstract><AbstractText>Elasmobranch and teleost fish have their Ig light (L) chain loci organized in multiple clusters (VL-JL-CL). The VL segments of teleosts are in opposite transcriptional orientation to the CL genes, suggesting that in teleosts and elasmobranchs there may have been separate evolutionary events leading to this organization. To address this problem, the IgL locus from the Siberian sturgeon (Acipenser baeri) (representative of a branch between elasmobranchs and teleosts) was investigated. Sequence analysis of cDNA clones shows that sturgeon VL genes are most similar to those of teleosts, but that sturgeon CL genes are more similar to those of the sharks. Southern blot analyses of sturgeon erythrocyte DNA with VL- and CL-specific probes showed that there are more than 20 VL segments in both the tetraploid Siberian sturgeon and the diploid sterlet (Acipenser ruthenus), but only a few CL segments in the genome of the Siberian sturgeon and up to four CL segments in that of the sterlet. Screening of an unamplified genomic library gave more than 300 VL-positive and four CL-positive clones. None of these contained inserts positive for both probes. PCR analysis of a genomic CL clone using IC and CL-specific primers suggested that upstream of the CL segment there are at least seven JL segments. it is concluded that sturgeons have a kappa-like organization of their IgL locus and that the clustered organization of IgL loci in bony fish and sharks arose from two distinct evolutionary events.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lundqvist</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology and Microbiology, BMC, Uppsala University, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bengtén</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Strömberg</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Pilström</LastName><ForeName>L</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>X90557</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Immunol</MedlineTA><NlmUniqueID>2985117R</NlmUniqueID><ISSNLinking>0022-1767</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical></ChemicalList><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000916" MajorTopicYN="N">Antibody Diversity</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="Y">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005803" MajorTopicYN="N">Genes, Immunoglobulin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>9</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8757324</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country><region><li>East Middle Sweden</li><li>Svealand</li></region><settlement><li>Uppsala</li></settlement><orgName><li>Université d'Uppsala</li></orgName></list><tree><noCountry><name sortKey="Bengten, E" sort="Bengten, E" uniqKey="Bengten E" first="E" last="Bengtén">E. Bengtén</name><name sortKey="Pilstrom, L" sort="Pilstrom, L" uniqKey="Pilstrom L" first="L" last="Pilström">L. Pilström</name><name sortKey="Stromberg, S" sort="Stromberg, S" uniqKey="Stromberg S" first="S" last="Strömberg">S. Strömberg</name></noCountry><country name="Suède"><region name="Svealand"><name sortKey="Lundqvist, M" sort="Lundqvist, M" uniqKey="Lundqvist M" first="M" last="Lundqvist">M. Lundqvist</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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