[Mitochondrial DNA length variation and heteroplasmy in Chinese sturgeon (Acipenser sinensis)].
Identifieur interne : 000608 ( PubMed/Checkpoint ); précédent : 000607; suivant : 000609[Mitochondrial DNA length variation and heteroplasmy in Chinese sturgeon (Acipenser sinensis)].
Auteurs : S M Zhang ; H. Deng ; D Q Wang ; Y P Zhang ; Q J WuSource :
- Yi chuan xue bao = Acta genetica Sinica [ 0379-4172 ] ; 1999.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Mitochondrial.
- genetics : Fishes.
- Animals, Base Sequence, Genetic Variation, Molecular Sequence Data, Tandem Repeat Sequences.
Abstract
The D-loop of mitochondrial DNA from Chinese sturgeon (Acipenser sinensis) was amplified using PCR. The length variations among individuals and within individual (heteroplasmy) were detected. DNA sequence analysis revealed that interindivadual and intraindividual length variation occurred within D-loop near tRNA(pro), and caused by tandam repeat with 82 bp in length. Out of 47 individuals, 27(57.4%) individuals were heteroplasmy whereas 20 individual (42.6%) were homoplasmic. The length variation was also found among homoplasmic individuals. In homoplasmic individual four different size classes (number of tandem repeats) e.g. 2, 3, 4 or 5 repeats were noted. The frequencies of size classes from the highest to the lowest were 3-->2-->4-->5. In heteroplasmy two molecular size classes coexisted in an individual was most common (77.78%). The second type of heteroplasmy was three size classes coexisted (18.52%) and the lowest one was four size classes together in an individual (3.70%). No five different size classes coexisted in an individual was found. Through densitometry of DNA bands the frequencies of mtDNA size classes were determined for the sample of individuals, and the frequency distribution of repeats from the highest to the lowest was similar to that in homoplasmy (3-->2-->4-->5-->1). The diversity within each heteroplasmic individual was carried out and genetic diversity at hierarchical level was analyzed. 65.3% of genetic diversities were from among individuals in population and 34.7% of genetic diversities were from within individual. The mtDNA length variation within individual (heteroplasmy) is another genetic diversity resource for Chinese sturgeon.
PubMed: 10665225
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Deng</name></author><author><name sortKey="Wang, D Q" sort="Wang, D Q" uniqKey="Wang D" first="D Q" last="Wang">D Q Wang</name></author><author><name sortKey="Zhang, Y P" sort="Zhang, Y P" uniqKey="Zhang Y" first="Y P" last="Zhang">Y P Zhang</name></author><author><name sortKey="Wu, Q J" sort="Wu, Q J" uniqKey="Wu Q" first="Q J" last="Wu">Q J Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10665225</idno><idno type="pmid">10665225</idno><idno type="wicri:Area/PubMed/Corpus">000664</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000664</idno><idno type="wicri:Area/PubMed/Curation">000664</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000664</idno><idno type="wicri:Area/PubMed/Checkpoint">000664</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000664</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Mitochondrial DNA length variation and heteroplasmy in Chinese sturgeon (Acipenser sinensis)].</title><author><name sortKey="Zhang, S M" sort="Zhang, S M" uniqKey="Zhang S" first="S M" last="Zhang">S M Zhang</name><affiliation><nlm:affiliation>Yangtze River Institute of Fisheries, Chinese Academy of Fishery Sciences, Jingzhou.</nlm:affiliation><wicri:noCountry code="subField">Jingzhou</wicri:noCountry></affiliation></author><author><name sortKey="Deng, H" sort="Deng, H" uniqKey="Deng H" first="H" last="Deng">H. Deng</name></author><author><name sortKey="Wang, D Q" sort="Wang, D Q" uniqKey="Wang D" first="D Q" last="Wang">D Q Wang</name></author><author><name sortKey="Zhang, Y P" sort="Zhang, Y P" uniqKey="Zhang Y" first="Y P" last="Zhang">Y P Zhang</name></author><author><name sortKey="Wu, Q J" sort="Wu, Q J" uniqKey="Wu Q" first="Q J" last="Wu">Q J Wu</name></author></analytic><series><title level="j">Yi chuan xue bao = Acta genetica Sinica</title><idno type="ISSN">0379-4172</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>DNA, Mitochondrial (chemistry)</term><term>Fishes (genetics)</term><term>Genetic Variation</term><term>Molecular Sequence Data</term><term>Tandem Repeat Sequences</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Mitochondrial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Genetic Variation</term><term>Molecular Sequence Data</term><term>Tandem Repeat Sequences</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The D-loop of mitochondrial DNA from Chinese sturgeon (Acipenser sinensis) was amplified using PCR. The length variations among individuals and within individual (heteroplasmy) were detected. DNA sequence analysis revealed that interindivadual and intraindividual length variation occurred within D-loop near tRNA(pro), and caused by tandam repeat with 82 bp in length. Out of 47 individuals, 27(57.4%) individuals were heteroplasmy whereas 20 individual (42.6%) were homoplasmic. The length variation was also found among homoplasmic individuals. In homoplasmic individual four different size classes (number of tandem repeats) e.g. 2, 3, 4 or 5 repeats were noted. The frequencies of size classes from the highest to the lowest were 3-->2-->4-->5. In heteroplasmy two molecular size classes coexisted in an individual was most common (77.78%). The second type of heteroplasmy was three size classes coexisted (18.52%) and the lowest one was four size classes together in an individual (3.70%). No five different size classes coexisted in an individual was found. Through densitometry of DNA bands the frequencies of mtDNA size classes were determined for the sample of individuals, and the frequency distribution of repeats from the highest to the lowest was similar to that in homoplasmy (3-->2-->4-->5-->1). The diversity within each heteroplasmic individual was carried out and genetic diversity at hierarchical level was analyzed. 65.3% of genetic diversities were from among individuals in population and 34.7% of genetic diversities were from within individual. The mtDNA length variation within individual (heteroplasmy) is another genetic diversity resource for Chinese sturgeon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10665225</PMID><DateCreated><Year>2000</Year><Month>02</Month><Day>24</Day></DateCreated><DateCompleted><Year>2000</Year><Month>02</Month><Day>24</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0379-4172</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>5</Issue><PubDate><Year>1999</Year></PubDate></JournalIssue><Title>Yi chuan xue bao = Acta genetica Sinica</Title><ISOAbbreviation>Yi Chuan Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Mitochondrial DNA length variation and heteroplasmy in Chinese sturgeon (Acipenser sinensis)].</ArticleTitle><Pagination><MedlinePgn>489-96</MedlinePgn></Pagination><Abstract><AbstractText>The D-loop of mitochondrial DNA from Chinese sturgeon (Acipenser sinensis) was amplified using PCR. The length variations among individuals and within individual (heteroplasmy) were detected. DNA sequence analysis revealed that interindivadual and intraindividual length variation occurred within D-loop near tRNA(pro), and caused by tandam repeat with 82 bp in length. Out of 47 individuals, 27(57.4%) individuals were heteroplasmy whereas 20 individual (42.6%) were homoplasmic. The length variation was also found among homoplasmic individuals. In homoplasmic individual four different size classes (number of tandem repeats) e.g. 2, 3, 4 or 5 repeats were noted. The frequencies of size classes from the highest to the lowest were 3-->2-->4-->5. In heteroplasmy two molecular size classes coexisted in an individual was most common (77.78%). The second type of heteroplasmy was three size classes coexisted (18.52%) and the lowest one was four size classes together in an individual (3.70%). No five different size classes coexisted in an individual was found. Through densitometry of DNA bands the frequencies of mtDNA size classes were determined for the sample of individuals, and the frequency distribution of repeats from the highest to the lowest was similar to that in homoplasmy (3-->2-->4-->5-->1). The diversity within each heteroplasmic individual was carried out and genetic diversity at hierarchical level was analyzed. 65.3% of genetic diversities were from among individuals in population and 34.7% of genetic diversities were from within individual. The mtDNA length variation within individual (heteroplasmy) is another genetic diversity resource for Chinese sturgeon.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>S M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Yangtze River Institute of Fisheries, Chinese Academy of Fishery Sciences, Jingzhou.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>D Q</ForeName><Initials>DQ</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Y P</ForeName><Initials>YP</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Q J</ForeName><Initials>QJ</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Yi Chuan Xue Bao</MedlineTA><NlmUniqueID>7900784</NlmUniqueID><ISSNLinking>0379-4172</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004272">DNA, Mitochondrial</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004272" MajorTopicYN="N">DNA, Mitochondrial</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020080" MajorTopicYN="N">Tandem Repeat Sequences</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>2</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>2</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>2</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10665225</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Deng, H" sort="Deng, H" uniqKey="Deng H" first="H" last="Deng">H. Deng</name><name sortKey="Wang, D Q" sort="Wang, D Q" uniqKey="Wang D" first="D Q" last="Wang">D Q Wang</name><name sortKey="Wu, Q J" sort="Wu, Q J" uniqKey="Wu Q" first="Q J" last="Wu">Q J Wu</name><name sortKey="Zhang, S M" sort="Zhang, S M" uniqKey="Zhang S" first="S M" last="Zhang">S M Zhang</name><name sortKey="Zhang, Y P" sort="Zhang, Y P" uniqKey="Zhang Y" first="Y P" last="Zhang">Y P Zhang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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