Heteroplasmy in the mtDNA control region of sturgeon (Acipenser, Huso and Scaphirhynchus).
Identifieur interne : 000598 ( PubMed/Checkpoint ); précédent : 000597; suivant : 000599Heteroplasmy in the mtDNA control region of sturgeon (Acipenser, Huso and Scaphirhynchus).
Auteurs : A. Ludwig [Allemagne] ; B. May ; L. Debus ; I. JenneckensSource :
- Genetics [ 0016-6731 ] ; 2000.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cytochrome b Group (genetics), DNA, Mitochondrial (genetics), DNA, Mitochondrial (ultrastructure), Evolution, Molecular, Female, Fishes (classification), Fishes (genetics), Hot Temperature, Male, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, Polymerase Chain Reaction, RNA, Transfer, Pro (genetics), Repetitive Sequences, Nucleic Acid, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity.
- MESH :
- chemical , genetics : Cytochrome b Group, DNA, Mitochondrial, RNA, Transfer, Pro.
- chemical , ultrastructure : DNA, Mitochondrial.
- classification : Fishes.
- genetics : Fishes.
- Animals, Base Sequence, Evolution, Molecular, Female, Hot Temperature, Male, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, Polymerase Chain Reaction, Repetitive Sequences, Nucleic Acid, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity.
Abstract
Data from 1238 fishes from 19 sturgeon species and 1 paddlefish were used to analyze heteroplasmy in sturgeon. Lengths of central repeat units ranged from 74 to 83 bp among sturgeon species. No repeat sequence was found in the paddlefish, Polyodon spathula. A general feature of the repeat units was the presence of termination associated sequence (TAS) motifs. About 50% of 138 interspecific mutations observed among the D-loop sequences are located 10 bp down- and upstream from these TAS motifs. Interestingly, most homoplasmic species showed deletions upstream to the TAS motifs, whereas deletions downstream to the TAS motifs observed in two species do not seem to preclude heteroplasmy. Calculations of secondary structures and thermal stabilities of repeat units showed DeltaG values for all heteroplasmic species to be <-8 and for most homoplasmic species DeltaG value to be >-8. Most heteroplasmic fishes had two and/or three repeat units. No homoplasmic sturgeon with >2 repeat units were observed. Molecular phylogeny based on the entire cytochrome b showed that heteroplasmy probably resulted from a single evolutionary event. Our data demonstrate that heteroplasmy is present in most sturgeon species and suggest that the thermal stability of the secondary structure of the repeat unit in combination with mutations downstream of the TAS sequences influences heteroplasmy.
PubMed: 11102385
Affiliations:
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Debus</name></author><author><name sortKey="Jenneckens, I" sort="Jenneckens, I" uniqKey="Jenneckens I" first="I" last="Jenneckens">I. Jenneckens</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:11102385</idno><idno type="pmid">11102385</idno><idno type="wicri:Area/PubMed/Corpus">000637</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000637</idno><idno type="wicri:Area/PubMed/Curation">000637</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000637</idno><idno type="wicri:Area/PubMed/Checkpoint">000637</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000637</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Heteroplasmy in the mtDNA control region of sturgeon (Acipenser, Huso and Scaphirhynchus).</title><author><name sortKey="Ludwig, A" sort="Ludwig, A" uniqKey="Ludwig A" first="A" last="Ludwig">A. Ludwig</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Freshwater Ecology and Inland Fisheries, 12561 Berlin, Germany. al@igb-berlin.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Freshwater Ecology and Inland Fisheries, 12561 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="May, B" sort="May, B" uniqKey="May B" first="B" last="May">B. May</name></author><author><name sortKey="Debus, L" sort="Debus, L" uniqKey="Debus L" first="L" last="Debus">L. Debus</name></author><author><name sortKey="Jenneckens, I" sort="Jenneckens, I" uniqKey="Jenneckens I" first="I" last="Jenneckens">I. Jenneckens</name></author></analytic><series><title level="j">Genetics</title><idno type="ISSN">0016-6731</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cytochrome b Group (genetics)</term><term>DNA, Mitochondrial (genetics)</term><term>DNA, Mitochondrial (ultrastructure)</term><term>Evolution, Molecular</term><term>Female</term><term>Fishes (classification)</term><term>Fishes (genetics)</term><term>Hot Temperature</term><term>Male</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>RNA, Transfer, Pro (genetics)</term><term>Repetitive Sequences, Nucleic Acid</term><term>Sequence Alignment</term><term>Sequence Homology, Nucleic Acid</term><term>Species Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytochrome b Group</term><term>DNA, Mitochondrial</term><term>RNA, Transfer, Pro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>DNA, Mitochondrial</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Evolution, Molecular</term><term>Female</term><term>Hot Temperature</term><term>Male</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Repetitive Sequences, Nucleic Acid</term><term>Sequence Alignment</term><term>Sequence Homology, Nucleic Acid</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Data from 1238 fishes from 19 sturgeon species and 1 paddlefish were used to analyze heteroplasmy in sturgeon. Lengths of central repeat units ranged from 74 to 83 bp among sturgeon species. No repeat sequence was found in the paddlefish, Polyodon spathula. A general feature of the repeat units was the presence of termination associated sequence (TAS) motifs. About 50% of 138 interspecific mutations observed among the D-loop sequences are located 10 bp down- and upstream from these TAS motifs. Interestingly, most homoplasmic species showed deletions upstream to the TAS motifs, whereas deletions downstream to the TAS motifs observed in two species do not seem to preclude heteroplasmy. Calculations of secondary structures and thermal stabilities of repeat units showed DeltaG values for all heteroplasmic species to be <-8 and for most homoplasmic species DeltaG value to be >-8. Most heteroplasmic fishes had two and/or three repeat units. No homoplasmic sturgeon with >2 repeat units were observed. Molecular phylogeny based on the entire cytochrome b showed that heteroplasmy probably resulted from a single evolutionary event. Our data demonstrate that heteroplasmy is present in most sturgeon species and suggest that the thermal stability of the secondary structure of the repeat unit in combination with mutations downstream of the TAS sequences influences heteroplasmy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11102385</PMID><DateCreated><Year>2000</Year><Month>12</Month><Day>28</Day></DateCreated><DateCompleted><Year>2001</Year><Month>02</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>06</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0016-6731</ISSN><JournalIssue CitedMedium="Print"><Volume>156</Volume><Issue>4</Issue><PubDate><Year>2000</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Genetics</Title><ISOAbbreviation>Genetics</ISOAbbreviation></Journal><ArticleTitle>Heteroplasmy in the mtDNA control region of sturgeon (Acipenser, Huso and Scaphirhynchus).</ArticleTitle><Pagination><MedlinePgn>1933-47</MedlinePgn></Pagination><Abstract><AbstractText>Data from 1238 fishes from 19 sturgeon species and 1 paddlefish were used to analyze heteroplasmy in sturgeon. Lengths of central repeat units ranged from 74 to 83 bp among sturgeon species. No repeat sequence was found in the paddlefish, Polyodon spathula. A general feature of the repeat units was the presence of termination associated sequence (TAS) motifs. About 50% of 138 interspecific mutations observed among the D-loop sequences are located 10 bp down- and upstream from these TAS motifs. Interestingly, most homoplasmic species showed deletions upstream to the TAS motifs, whereas deletions downstream to the TAS motifs observed in two species do not seem to preclude heteroplasmy. Calculations of secondary structures and thermal stabilities of repeat units showed DeltaG values for all heteroplasmic species to be <-8 and for most homoplasmic species DeltaG value to be >-8. Most heteroplasmic fishes had two and/or three repeat units. No homoplasmic sturgeon with >2 repeat units were observed. Molecular phylogeny based on the entire cytochrome b showed that heteroplasmy probably resulted from a single evolutionary event. Our data demonstrate that heteroplasmy is present in most sturgeon species and suggest that the thermal stability of the secondary structure of the repeat unit in combination with mutations downstream of the TAS sequences influences heteroplasmy.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ludwig</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Freshwater Ecology and Inland Fisheries, 12561 Berlin, Germany. al@igb-berlin.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>May</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Debus</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Jenneckens</LastName><ForeName>I</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Genetics</MedlineTA><NlmUniqueID>0374636</NlmUniqueID><ISSNLinking>0016-6731</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003573">Cytochrome b Group</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004272">DNA, Mitochondrial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012361">RNA, Transfer, Pro</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Anim Genet. 2000 Apr;31(2):153-4</RefSource><PMID Version="1">10782231</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Phylogenet Evol. 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UI="D004272" MajorTopicYN="N">DNA, Mitochondrial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009690" MajorTopicYN="N">Nucleic Acid Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012361" MajorTopicYN="N">RNA, Transfer, Pro</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012091" MajorTopicYN="N">Repetitive Sequences, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC1461359</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>12</Month><Day>5</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>3</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>12</Month><Day>5</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11102385</ArticleId><ArticleId IdType="pmc">PMC1461359</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><noCountry><name sortKey="Debus, L" sort="Debus, L" uniqKey="Debus L" first="L" last="Debus">L. Debus</name><name sortKey="Jenneckens, I" sort="Jenneckens, I" uniqKey="Jenneckens I" first="I" last="Jenneckens">I. Jenneckens</name><name sortKey="May, B" sort="May, B" uniqKey="May B" first="B" last="May">B. May</name></noCountry><country name="Allemagne"><region name="Berlin"><name sortKey="Ludwig, A" sort="Ludwig, A" uniqKey="Ludwig A" first="A" last="Ludwig">A. Ludwig</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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