Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor?
Identifieur interne : 000580 ( PubMed/Checkpoint ); précédent : 000579; suivant : 000581Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor?
Auteurs : L. Langouche [Belgique] ; M. Roudbaraki ; K. Pals ; C. DenefSource :
- Endocrinology [ 0013-7227 ] ; 2001.
English descriptors
- KwdEn :
- Adrenal Glands (metabolism), Animals, Brain (metabolism), CHO Cells, Calcium (metabolism), Cell Line, Cricetinae, Cyclic AMP (metabolism), Humans, Melanocyte-Stimulating Hormones (pharmacology), Oligopeptides (pharmacology), Pituitary Gland, Rats, Receptor, Melanocortin, Type 3, Receptor, Melanocortin, Type 4, Receptors, Corticotropin (drug effects), Receptors, Corticotropin (genetics), Receptors, Corticotropin (physiology), Recombinant Proteins (pharmacology), Reverse Transcriptase Polymerase Chain Reaction, Thyrotropin-Releasing Hormone (pharmacology), Transfection, alpha-MSH (analogs & derivatives), gamma-MSH (pharmacology), gamma-MSH (physiology).
- MESH :
- chemical , analogs & derivatives : alpha-MSH.
- chemical , drug effects : Receptors, Corticotropin.
- chemical , genetics : Receptors, Corticotropin.
- chemical , metabolism : Calcium, Cyclic AMP.
- metabolism : Adrenal Glands, Brain.
- chemical , pharmacology : Melanocyte-Stimulating Hormones, Oligopeptides, Recombinant Proteins, Thyrotropin-Releasing Hormone, gamma-MSH.
- chemical , physiology : Receptors, Corticotropin, gamma-MSH.
- Animals, CHO Cells, Cell Line, Cricetinae, Humans, Pituitary Gland, Rats, Receptor, Melanocortin, Type 3, Receptor, Melanocortin, Type 4, Reverse Transcriptase Polymerase Chain Reaction, Transfection.
Abstract
The melanocortin (MC) gamma3MSH is a peptide that can be generated from the N-terminal domain of POMC and is believed to signal through the MC3 receptor. We recently showed that it induces a sustained rise in intracellular free calcium levels ([Ca(2+)](i)) in a subpopulation of pituitary cells, particularly in the lactosomatotroph lineage. In the present study we report that gamma3MSH and some analogs increase [Ca(2+)](i) in the GH- and PRL-secreting GH3 cell line and evaluate on the basis of pharmacological experiments and gene expression studies which MC receptor may be involved. A dose as low as 1 pM gamma3MSH induced an oscillating [Ca(2+)](i) increase in a significant percentage of GH3 cells. Increasing the dose recruited an increasing number of responding cells; a maximum was reached at 0.1 nM. gamma2MSH, alphaMSH, and NDP-alphaMSH displayed a similar effect. SHU9119, an MC3 and MC4 receptor antagonist, and an MC5 receptor agonist, did not affect the number of cells showing a [Ca(2+)](i) rise in response to gamma3MSH. SHU9119 had also no effect when added alone. MTII, a potent synthetic agonist of the MC3, MC4, and MC5 receptor as well as an N-terminally extended recombinant analog of gamma3MSH showed low potency in increasing [Ca(2+)](i) in GH3 cells, but high potency in stimulating cAMP accumulation in HEK 293 cells stably transfected with the MC3 receptor. In contrast, a peptide corresponding to the gamma2MSH sequence of POMC-A of Acipenser transmontanus increased [Ca(2+)](i) in GH3 cells, but was about 50 times less potent than gamma2- or gamma3MSH in stimulating cAMP accumulation in the MC3 receptor expressing HEK 293 cells. By means of RT-PCR performed on a RNA extract from GH3 cells, the messenger RNA of the MC2, MC3, and MC4 receptor was undetectable, but messenger RNA of the MC5 receptor was clearly present. These data suggest that the GH3 cell line does not mediate the effect of gamma3MSH through the MC3 receptor. The involvement of the MC5 receptor is unlikely, but cannot definitely be excluded. The findings animate the hypothesis that there exists a second, hitherto unidentified, MC receptor that displays high affinity for gamma3MSH.
DOI: 10.1210/endo.142.1.7878
PubMed: 11145589
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:11145589Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor?</title>
<author><name sortKey="Langouche, L" sort="Langouche, L" uniqKey="Langouche L" first="L" last="Langouche">L. Langouche</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cell Pharmacology, University of Leuven, Medical School, Campus Gasthuisberg, B-3000 Leuven, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Cell Pharmacology, University of Leuven, Medical School, Campus Gasthuisberg, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Roudbaraki, M" sort="Roudbaraki, M" uniqKey="Roudbaraki M" first="M" last="Roudbaraki">M. Roudbaraki</name>
</author>
<author><name sortKey="Pals, K" sort="Pals, K" uniqKey="Pals K" first="K" last="Pals">K. Pals</name>
</author>
<author><name sortKey="Denef, C" sort="Denef, C" uniqKey="Denef C" first="C" last="Denef">C. Denef</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2001">2001</date>
<idno type="RBID">pubmed:11145589</idno>
<idno type="pmid">11145589</idno>
<idno type="doi">10.1210/endo.142.1.7878</idno>
<idno type="wicri:Area/PubMed/Corpus">000631</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000631</idno>
<idno type="wicri:Area/PubMed/Curation">000631</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000631</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000631</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000631</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor?</title>
<author><name sortKey="Langouche, L" sort="Langouche, L" uniqKey="Langouche L" first="L" last="Langouche">L. Langouche</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cell Pharmacology, University of Leuven, Medical School, Campus Gasthuisberg, B-3000 Leuven, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Cell Pharmacology, University of Leuven, Medical School, Campus Gasthuisberg, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Roudbaraki, M" sort="Roudbaraki, M" uniqKey="Roudbaraki M" first="M" last="Roudbaraki">M. Roudbaraki</name>
</author>
<author><name sortKey="Pals, K" sort="Pals, K" uniqKey="Pals K" first="K" last="Pals">K. Pals</name>
</author>
<author><name sortKey="Denef, C" sort="Denef, C" uniqKey="Denef C" first="C" last="Denef">C. Denef</name>
</author>
</analytic>
<series><title level="j">Endocrinology</title>
<idno type="ISSN">0013-7227</idno>
<imprint><date when="2001" type="published">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adrenal Glands (metabolism)</term>
<term>Animals</term>
<term>Brain (metabolism)</term>
<term>CHO Cells</term>
<term>Calcium (metabolism)</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Cyclic AMP (metabolism)</term>
<term>Humans</term>
<term>Melanocyte-Stimulating Hormones (pharmacology)</term>
<term>Oligopeptides (pharmacology)</term>
<term>Pituitary Gland</term>
<term>Rats</term>
<term>Receptor, Melanocortin, Type 3</term>
<term>Receptor, Melanocortin, Type 4</term>
<term>Receptors, Corticotropin (drug effects)</term>
<term>Receptors, Corticotropin (genetics)</term>
<term>Receptors, Corticotropin (physiology)</term>
<term>Recombinant Proteins (pharmacology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Thyrotropin-Releasing Hormone (pharmacology)</term>
<term>Transfection</term>
<term>alpha-MSH (analogs & derivatives)</term>
<term>gamma-MSH (pharmacology)</term>
<term>gamma-MSH (physiology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>alpha-MSH</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Receptors, Corticotropin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptors, Corticotropin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term>
<term>Cyclic AMP</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Adrenal Glands</term>
<term>Brain</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Melanocyte-Stimulating Hormones</term>
<term>Oligopeptides</term>
<term>Recombinant Proteins</term>
<term>Thyrotropin-Releasing Hormone</term>
<term>gamma-MSH</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Receptors, Corticotropin</term>
<term>gamma-MSH</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>CHO Cells</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Humans</term>
<term>Pituitary Gland</term>
<term>Rats</term>
<term>Receptor, Melanocortin, Type 3</term>
<term>Receptor, Melanocortin, Type 4</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Transfection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The melanocortin (MC) gamma3MSH is a peptide that can be generated from the N-terminal domain of POMC and is believed to signal through the MC3 receptor. We recently showed that it induces a sustained rise in intracellular free calcium levels ([Ca(2+)](i)) in a subpopulation of pituitary cells, particularly in the lactosomatotroph lineage. In the present study we report that gamma3MSH and some analogs increase [Ca(2+)](i) in the GH- and PRL-secreting GH3 cell line and evaluate on the basis of pharmacological experiments and gene expression studies which MC receptor may be involved. A dose as low as 1 pM gamma3MSH induced an oscillating [Ca(2+)](i) increase in a significant percentage of GH3 cells. Increasing the dose recruited an increasing number of responding cells; a maximum was reached at 0.1 nM. gamma2MSH, alphaMSH, and NDP-alphaMSH displayed a similar effect. SHU9119, an MC3 and MC4 receptor antagonist, and an MC5 receptor agonist, did not affect the number of cells showing a [Ca(2+)](i) rise in response to gamma3MSH. SHU9119 had also no effect when added alone. MTII, a potent synthetic agonist of the MC3, MC4, and MC5 receptor as well as an N-terminally extended recombinant analog of gamma3MSH showed low potency in increasing [Ca(2+)](i) in GH3 cells, but high potency in stimulating cAMP accumulation in HEK 293 cells stably transfected with the MC3 receptor. In contrast, a peptide corresponding to the gamma2MSH sequence of POMC-A of Acipenser transmontanus increased [Ca(2+)](i) in GH3 cells, but was about 50 times less potent than gamma2- or gamma3MSH in stimulating cAMP accumulation in the MC3 receptor expressing HEK 293 cells. By means of RT-PCR performed on a RNA extract from GH3 cells, the messenger RNA of the MC2, MC3, and MC4 receptor was undetectable, but messenger RNA of the MC5 receptor was clearly present. These data suggest that the GH3 cell line does not mediate the effect of gamma3MSH through the MC3 receptor. The involvement of the MC5 receptor is unlikely, but cannot definitely be excluded. The findings animate the hypothesis that there exists a second, hitherto unidentified, MC receptor that displays high affinity for gamma3MSH.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11145589</PMID>
<DateCreated><Year>2001</Year>
<Month>01</Month>
<Day>26</Day>
</DateCreated>
<DateCompleted><Year>2001</Year>
<Month>03</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0013-7227</ISSN>
<JournalIssue CitedMedium="Print"><Volume>142</Volume>
<Issue>1</Issue>
<PubDate><Year>2001</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Endocrinology</Title>
<ISOAbbreviation>Endocrinology</ISOAbbreviation>
</Journal>
<ArticleTitle>Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor?</ArticleTitle>
<Pagination><MedlinePgn>257-66</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The melanocortin (MC) gamma3MSH is a peptide that can be generated from the N-terminal domain of POMC and is believed to signal through the MC3 receptor. We recently showed that it induces a sustained rise in intracellular free calcium levels ([Ca(2+)](i)) in a subpopulation of pituitary cells, particularly in the lactosomatotroph lineage. In the present study we report that gamma3MSH and some analogs increase [Ca(2+)](i) in the GH- and PRL-secreting GH3 cell line and evaluate on the basis of pharmacological experiments and gene expression studies which MC receptor may be involved. A dose as low as 1 pM gamma3MSH induced an oscillating [Ca(2+)](i) increase in a significant percentage of GH3 cells. Increasing the dose recruited an increasing number of responding cells; a maximum was reached at 0.1 nM. gamma2MSH, alphaMSH, and NDP-alphaMSH displayed a similar effect. SHU9119, an MC3 and MC4 receptor antagonist, and an MC5 receptor agonist, did not affect the number of cells showing a [Ca(2+)](i) rise in response to gamma3MSH. SHU9119 had also no effect when added alone. MTII, a potent synthetic agonist of the MC3, MC4, and MC5 receptor as well as an N-terminally extended recombinant analog of gamma3MSH showed low potency in increasing [Ca(2+)](i) in GH3 cells, but high potency in stimulating cAMP accumulation in HEK 293 cells stably transfected with the MC3 receptor. In contrast, a peptide corresponding to the gamma2MSH sequence of POMC-A of Acipenser transmontanus increased [Ca(2+)](i) in GH3 cells, but was about 50 times less potent than gamma2- or gamma3MSH in stimulating cAMP accumulation in the MC3 receptor expressing HEK 293 cells. By means of RT-PCR performed on a RNA extract from GH3 cells, the messenger RNA of the MC2, MC3, and MC4 receptor was undetectable, but messenger RNA of the MC5 receptor was clearly present. These data suggest that the GH3 cell line does not mediate the effect of gamma3MSH through the MC3 receptor. The involvement of the MC5 receptor is unlikely, but cannot definitely be excluded. The findings animate the hypothesis that there exists a second, hitherto unidentified, MC receptor that displays high affinity for gamma3MSH.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Langouche</LastName>
<ForeName>L</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Laboratory of Cell Pharmacology, University of Leuven, Medical School, Campus Gasthuisberg, B-3000 Leuven, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Roudbaraki</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Pals</LastName>
<ForeName>K</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Denef</LastName>
<ForeName>C</ForeName>
<Initials>C</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Endocrinology</MedlineTA>
<NlmUniqueID>0375040</NlmUniqueID>
<ISSNLinking>0013-7227</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009842">Oligopeptides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D044104">Receptor, Melanocortin, Type 3</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D044105">Receptor, Melanocortin, Type 4</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018043">Receptors, Corticotropin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C105974">acetyl-norleucyl(4)-(aspartyl(5)-histidyl(6)-phenylalanyl(7)-arginyl(8)-tryptophyl(9)-lysyl(10))cyclo-alpha-MSH(4-10)amide</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019825">gamma-MSH</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>168482-23-3</RegistryNumber>
<NameOfSubstance UI="C103272">SHU 9119</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>581-05-5</RegistryNumber>
<NameOfSubstance UI="D000521">alpha-MSH</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>5Y5F15120W</RegistryNumber>
<NameOfSubstance UI="D013973">Thyrotropin-Releasing Hormone</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9002-79-3</RegistryNumber>
<NameOfSubstance UI="D009074">Melanocyte-Stimulating Hormones</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>E0399OZS9N</RegistryNumber>
<NameOfSubstance UI="D000242">Cyclic AMP</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber>
<NameOfSubstance UI="D002118">Calcium</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>AIM</CitationSubset>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000311" MajorTopicYN="N">Adrenal Glands</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001921" MajorTopicYN="N">Brain</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016466" MajorTopicYN="N">CHO Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002118" MajorTopicYN="N">Calcium</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000242" MajorTopicYN="N">Cyclic AMP</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009074" MajorTopicYN="N">Melanocyte-Stimulating Hormones</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009842" MajorTopicYN="N">Oligopeptides</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010902" MajorTopicYN="N">Pituitary Gland</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D044104" MajorTopicYN="N">Receptor, Melanocortin, Type 3</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D044105" MajorTopicYN="N">Receptor, Melanocortin, Type 4</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018043" MajorTopicYN="N">Receptors, Corticotropin</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013973" MajorTopicYN="N">Thyrotropin-Releasing Hormone</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000521" MajorTopicYN="N">alpha-MSH</DescriptorName>
<QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019825" MajorTopicYN="N">gamma-MSH</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year>
<Month>1</Month>
<Day>6</Day>
<Hour>11</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2001</Year>
<Month>3</Month>
<Day>7</Day>
<Hour>10</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2001</Year>
<Month>1</Month>
<Day>6</Day>
<Hour>11</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">11145589</ArticleId>
<ArticleId IdType="doi">10.1210/endo.142.1.7878</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Belgique</li>
</country>
</list>
<tree><noCountry><name sortKey="Denef, C" sort="Denef, C" uniqKey="Denef C" first="C" last="Denef">C. Denef</name>
<name sortKey="Pals, K" sort="Pals, K" uniqKey="Pals K" first="K" last="Pals">K. Pals</name>
<name sortKey="Roudbaraki, M" sort="Roudbaraki, M" uniqKey="Roudbaraki M" first="M" last="Roudbaraki">M. Roudbaraki</name>
</noCountry>
<country name="Belgique"><noRegion><name sortKey="Langouche, L" sort="Langouche, L" uniqKey="Langouche L" first="L" last="Langouche">L. Langouche</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000580 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000580 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:11145589 |texte= Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor? }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:11145589" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |