Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus.
Identifieur interne : 000545 ( PubMed/Checkpoint ); précédent : 000544; suivant : 000546Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus.
Auteurs : Guitang Wang [États-Unis] ; Scott Lapatra ; Lingbing Zeng ; Zhengshan Zhao ; Yuanan LuSource :
- Methods in cell science : an official journal of the Society for In Vitro Biology [ 1381-5741 ] ; 2003.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA.
- chemical : Culture Media.
- cytology : Muscles.
- genetics : Chromosomes, Fishes.
- metabolism : Muscles.
- methods : Cryopreservation, Karyotyping.
- Animals, Base Sequence, Cells, Cultured, Molecular Sequence Data.
Abstract
Three cell lines derived from fin (WSF), head soft tissue (WSHST) and body muscle (WSBM) were established from white sturgeon (Acipenser transmontanus). Characterization included determination of optimal growth kinetics, karyotyping, and mitochondrial ribosomal RNA (rRNA) genotyping. The primary cultures of these cells were generated by the explant technique using the L-15 medium supplemented with 20% fetal bovine serum and epidermal/fibroblast growth factors. The cells grew between 15-30 degrees C, but optimal growth occurred at 25 degrees C with a doubling time of 48 hours. The cell lines can be readily maintained in-vitro and have been subcultured over 35 times. Following cryopreservation in liquid nitrogen, thawed cells exhibit a viability of > 90% after a 16-month storage period. Chromosomal typing of these cell lines at their 17th passage revealed a chromosomal distribution of 242 to 278 with an apparent peak ranging from 250 to 260. Polymerase chain reaction amplification of mitochondrial 16S ribosomal RNA and sequence analysis indicated 100% identity of the sequences found in the cell lines with those found in the source animal, confirming that the cell lines were of A. transmontanus origin.
PubMed: 15801167
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Lingbing" uniqKey="Zeng L" first="Lingbing" last="Zeng">Lingbing Zeng</name></author><author><name sortKey="Zhao, Zhengshan" sort="Zhao, Zhengshan" uniqKey="Zhao Z" first="Zhengshan" last="Zhao">Zhengshan Zhao</name></author><author><name sortKey="Lu, Yuanan" sort="Lu, Yuanan" uniqKey="Lu Y" first="Yuanan" last="Lu">Yuanan Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:15801167</idno><idno type="pmid">15801167</idno><idno type="wicri:Area/PubMed/Corpus">000592</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000592</idno><idno type="wicri:Area/PubMed/Curation">000592</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000592</idno><idno type="wicri:Area/PubMed/Checkpoint">000592</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" 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sortKey="Zeng, Lingbing" sort="Zeng, Lingbing" uniqKey="Zeng L" first="Lingbing" last="Zeng">Lingbing Zeng</name></author><author><name sortKey="Zhao, Zhengshan" sort="Zhao, Zhengshan" uniqKey="Zhao Z" first="Zhengshan" last="Zhao">Zhengshan Zhao</name></author><author><name sortKey="Lu, Yuanan" sort="Lu, Yuanan" uniqKey="Lu Y" first="Yuanan" last="Lu">Yuanan Lu</name></author></analytic><series><title level="j">Methods in cell science : an official journal of the Society for In Vitro Biology</title><idno type="ISSN">1381-5741</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cells, Cultured</term><term>Chromosomes (genetics)</term><term>Cryopreservation (methods)</term><term>Culture Media</term><term>Fishes (genetics)</term><term>Karyotyping (methods)</term><term>Molecular Sequence Data</term><term>Muscles (cytology)</term><term>Muscles (metabolism)</term><term>RNA (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Muscles</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes</term><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Muscles</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term><term>Karyotyping</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cells, Cultured</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three cell lines derived from fin (WSF), head soft tissue (WSHST) and body muscle (WSBM) were established from white sturgeon (Acipenser transmontanus). Characterization included determination of optimal growth kinetics, karyotyping, and mitochondrial ribosomal RNA (rRNA) genotyping. The primary cultures of these cells were generated by the explant technique using the L-15 medium supplemented with 20% fetal bovine serum and epidermal/fibroblast growth factors. The cells grew between 15-30 degrees C, but optimal growth occurred at 25 degrees C with a doubling time of 48 hours. The cell lines can be readily maintained in-vitro and have been subcultured over 35 times. Following cryopreservation in liquid nitrogen, thawed cells exhibit a viability of > 90% after a 16-month storage period. Chromosomal typing of these cell lines at their 17th passage revealed a chromosomal distribution of 242 to 278 with an apparent peak ranging from 250 to 260. Polymerase chain reaction amplification of mitochondrial 16S ribosomal RNA and sequence analysis indicated 100% identity of the sequences found in the cell lines with those found in the source animal, confirming that the cell lines were of A. transmontanus origin.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15801167</PMID><DateCreated><Year>2005</Year><Month>04</Month><Day>01</Day></DateCreated><DateCompleted><Year>2005</Year><Month>04</Month><Day>25</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1381-5741</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>3-4</Issue><PubDate><Year>2003</Year></PubDate></JournalIssue><Title>Methods in cell science : an official journal of the Society for In Vitro Biology</Title><ISOAbbreviation>Methods Cell Sci</ISOAbbreviation></Journal><ArticleTitle>Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus.</ArticleTitle><Pagination><MedlinePgn>211-20</MedlinePgn></Pagination><Abstract><AbstractText>Three cell lines derived from fin (WSF), head soft tissue (WSHST) and body muscle (WSBM) were established from white sturgeon (Acipenser transmontanus). Characterization included determination of optimal growth kinetics, karyotyping, and mitochondrial ribosomal RNA (rRNA) genotyping. The primary cultures of these cells were generated by the explant technique using the L-15 medium supplemented with 20% fetal bovine serum and epidermal/fibroblast growth factors. The cells grew between 15-30 degrees C, but optimal growth occurred at 25 degrees C with a doubling time of 48 hours. The cell lines can be readily maintained in-vitro and have been subcultured over 35 times. Following cryopreservation in liquid nitrogen, thawed cells exhibit a viability of > 90% after a 16-month storage period. Chromosomal typing of these cell lines at their 17th passage revealed a chromosomal distribution of 242 to 278 with an apparent peak ranging from 250 to 260. 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