Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches.
Identifieur interne : 000456 ( PubMed/Checkpoint ); précédent : 000455; suivant : 000457Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches.
Auteurs : Maria Sen Mun Wai [République populaire de Chine] ; Dietrich Ernst Lorke ; Aiqun Zhang ; Hsiang-Fu Kung ; David T. YewSource :
- Microscopy research and technique [ 1059-910X ] ; 2007.
English descriptors
- KwdEn :
- MESH :
- anatomy & histology : Goldfish, Spinal Cord, Spinal Nerve Roots.
- cytology : Central Nervous System.
- enzymology : Central Nervous System.
- physiology : Goldfish.
- Animals, Immunohistochemistry, Microscopy, Electron, Scanning.
Abstract
Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two-dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y-shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. gamma-aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5-HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5-HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two-dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord.
DOI: 10.1002/jemt.20515
PubMed: 17868134
Affiliations:
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Yew</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17868134</idno><idno type="pmid">17868134</idno><idno type="doi">10.1002/jemt.20515</idno><idno type="wicri:Area/PubMed/Corpus">000480</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000480</idno><idno type="wicri:Area/PubMed/Curation">000480</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000480</idno><idno type="wicri:Area/PubMed/Checkpoint">000480</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000480</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches.</title><author><name sortKey="Wai, Maria Sen Mun" sort="Wai, Maria Sen Mun" uniqKey="Wai M" first="Maria Sen Mun" last="Wai">Maria Sen Mun Wai</name><affiliation wicri:level="4"><nlm:affiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Lorke, Dietrich Ernst" sort="Lorke, Dietrich Ernst" uniqKey="Lorke D" first="Dietrich Ernst" last="Lorke">Dietrich Ernst Lorke</name></author><author><name sortKey="Zhang, Aiqun" sort="Zhang, Aiqun" uniqKey="Zhang A" first="Aiqun" last="Zhang">Aiqun Zhang</name></author><author><name sortKey="Kung, Hsiang Fu" sort="Kung, Hsiang Fu" uniqKey="Kung H" first="Hsiang-Fu" last="Kung">Hsiang-Fu Kung</name></author><author><name sortKey="Yew, David T" sort="Yew, David T" uniqKey="Yew D" first="David T" last="Yew">David T. Yew</name></author></analytic><series><title level="j">Microscopy research and technique</title><idno type="ISSN">1059-910X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Central Nervous System (cytology)</term><term>Central Nervous System (enzymology)</term><term>Goldfish (anatomy & histology)</term><term>Goldfish (physiology)</term><term>Immunohistochemistry</term><term>Microscopy, Electron, Scanning</term><term>Spinal Cord (anatomy & histology)</term><term>Spinal Nerve Roots (anatomy & histology)</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Goldfish</term><term>Spinal Cord</term><term>Spinal Nerve Roots</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Central Nervous System</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Central Nervous System</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Goldfish</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Immunohistochemistry</term><term>Microscopy, Electron, Scanning</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two-dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y-shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. gamma-aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5-HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5-HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two-dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17868134</PMID><DateCreated><Year>2007</Year><Month>11</Month><Day>21</Day></DateCreated><DateCompleted><Year>2008</Year><Month>03</Month><Day>14</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1059-910X</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>12</Issue><PubDate><Year>2007</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Microscopy research and technique</Title><ISOAbbreviation>Microsc. Res. Tech.</ISOAbbreviation></Journal><ArticleTitle>Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches.</ArticleTitle><Pagination><MedlinePgn>1079-90</MedlinePgn></Pagination><Abstract><AbstractText>Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two-dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y-shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. gamma-aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5-HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5-HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two-dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wai</LastName><ForeName>Maria Sen Mun</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lorke</LastName><ForeName>Dietrich Ernst</ForeName><Initials>DE</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Aiqun</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kung</LastName><ForeName>Hsiang-Fu</ForeName><Initials>HF</Initials></Author><Author ValidYN="Y"><LastName>Yew</LastName><ForeName>David T</ForeName><Initials>DT</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microsc Res Tech</MedlineTA><NlmUniqueID>9203012</NlmUniqueID><ISSNLinking>1059-910X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002490" MajorTopicYN="N">Central Nervous System</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006054" MajorTopicYN="N">Goldfish</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008855" MajorTopicYN="N">Microscopy, Electron, Scanning</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013116" MajorTopicYN="N">Spinal Cord</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013126" MajorTopicYN="N">Spinal Nerve Roots</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>9</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>3</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>9</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17868134</ArticleId><ArticleId IdType="doi">10.1002/jemt.20515</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><noCountry><name sortKey="Kung, Hsiang Fu" sort="Kung, Hsiang Fu" uniqKey="Kung H" first="Hsiang-Fu" last="Kung">Hsiang-Fu Kung</name><name sortKey="Lorke, Dietrich Ernst" sort="Lorke, Dietrich Ernst" uniqKey="Lorke D" first="Dietrich Ernst" last="Lorke">Dietrich Ernst Lorke</name><name sortKey="Yew, David T" sort="Yew, David T" uniqKey="Yew D" first="David T" last="Yew">David T. Yew</name><name sortKey="Zhang, Aiqun" sort="Zhang, Aiqun" uniqKey="Zhang A" first="Aiqun" last="Zhang">Aiqun Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wai, Maria Sen Mun" sort="Wai, Maria Sen Mun" uniqKey="Wai M" first="Maria Sen Mun" last="Wai">Maria Sen Mun Wai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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