Isolation and characterization of chondroitin sulfates from sturgeon (Acipenser sinensis) and their effects on growth of fibroblasts.
Identifieur interne : 000377 ( PubMed/Checkpoint ); précédent : 000376; suivant : 000378Isolation and characterization of chondroitin sulfates from sturgeon (Acipenser sinensis) and their effects on growth of fibroblasts.
Auteurs : A-Rang Im [Corée du Sud] ; Youmie Park ; Yeong Shik KimSource :
- Biological & pharmaceutical bulletin [ 1347-5215 ] ; 2010.
English descriptors
- KwdEn :
- Animals, Bone and Bones (chemistry), Cartilage (chemistry), Cell Adhesion (drug effects), Cell Line, Cell Movement (drug effects), Cell Proliferation (drug effects), Chondroitin Sulfates (isolation & purification), Chondroitin Sulfates (pharmacology), Chromatography, High Pressure Liquid, Fibroblasts (cytology), Fibroblasts (drug effects), Fibroblasts (enzymology), Fishes, Humans, Mitogen-Activated Protein Kinases (metabolism), Molecular Weight, Wound Healing (drug effects).
- MESH :
- chemical , isolation & purification : Chondroitin Sulfates.
- chemistry : Bone and Bones, Cartilage.
- cytology : Fibroblasts.
- drug effects : Cell Adhesion, Cell Movement, Cell Proliferation, Fibroblasts, Wound Healing.
- enzymology : Fibroblasts.
- chemical , metabolism : Mitogen-Activated Protein Kinases.
- chemical , pharmacology : Chondroitin Sulfates.
- Animals, Cell Line, Chromatography, High Pressure Liquid, Fishes, Humans, Molecular Weight.
Abstract
Chondroitin sulfate (CS) is a glycosaminoglycan that composed of hexosamine (D-galactosamine) and hexuronic acid (D-glucuronic acid) unit arranged in an alternating unbranched sequence. CS is an essential component of the extracellular matrix (ECM) of connective tissue. It is mainly covalently attached to core proteins in the form of proteoglycans so that it exhibits specific interactions with proteins for cell growth, differentiation, division and migration. In this study, CSs were purified from the cartilage and backbone of sturgeon (Acipenser sinensis). To characterize their biochemical properties, we performed disaccharide compositional analysis after chondroitinase ABC digestion, high performance size exclusion chromatography (HPSEC) and (1)H-NMR spectroscopy. We also investigated the effects of CSs on fibroblast proliferation and adhesion to determine whether wound healing was accelerated in vitro and proliferation of different mitogen-activated protein kinases (MAPK) signaling pathways was facilitated. The CS purified from sturgeon cartilage was primarily composed of 4-sulfated CS (88.8%) and sturgeon backbone CS contains more than 60% 6-sulfated CS. The average molecular weights of CSs obtained from sturgeon cartilage and backbone were found to be 8 and 43 kDa, respectively. Our results showed that both CSs are able to increase cell adhesion, induce proliferation and migration on fibroblasts and may accelerate wound healing by inducing MAPK signaling pathways.
PubMed: 20686217
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Im</name><affiliation wicri:level="4"><nlm:affiliation>Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Park, Youmie" sort="Park, Youmie" uniqKey="Park Y" first="Youmie" last="Park">Youmie Park</name></author><author><name sortKey="Kim, Yeong Shik" sort="Kim, Yeong Shik" uniqKey="Kim Y" first="Yeong Shik" last="Kim">Yeong Shik Kim</name></author></analytic><series><title level="j">Biological & pharmaceutical bulletin</title><idno type="eISSN">1347-5215</idno><imprint><date when="2010" 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qualifier="chemistry" xml:lang="en"><term>Bone and Bones</term><term>Cartilage</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Fibroblasts</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Adhesion</term><term>Cell Movement</term><term>Cell Proliferation</term><term>Fibroblasts</term><term>Wound Healing</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Fibroblasts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chondroitin Sulfates</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chromatography, High Pressure Liquid</term><term>Fishes</term><term>Humans</term><term>Molecular Weight</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chondroitin sulfate (CS) is a glycosaminoglycan that composed of hexosamine (D-galactosamine) and hexuronic acid (D-glucuronic acid) unit arranged in an alternating unbranched sequence. CS is an essential component of the extracellular matrix (ECM) of connective tissue. It is mainly covalently attached to core proteins in the form of proteoglycans so that it exhibits specific interactions with proteins for cell growth, differentiation, division and migration. In this study, CSs were purified from the cartilage and backbone of sturgeon (Acipenser sinensis). To characterize their biochemical properties, we performed disaccharide compositional analysis after chondroitinase ABC digestion, high performance size exclusion chromatography (HPSEC) and (1)H-NMR spectroscopy. We also investigated the effects of CSs on fibroblast proliferation and adhesion to determine whether wound healing was accelerated in vitro and proliferation of different mitogen-activated protein kinases (MAPK) signaling pathways was facilitated. The CS purified from sturgeon cartilage was primarily composed of 4-sulfated CS (88.8%) and sturgeon backbone CS contains more than 60% 6-sulfated CS. The average molecular weights of CSs obtained from sturgeon cartilage and backbone were found to be 8 and 43 kDa, respectively. Our results showed that both CSs are able to increase cell adhesion, induce proliferation and migration on fibroblasts and may accelerate wound healing by inducing MAPK signaling pathways.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20686217</PMID><DateCreated><Year>2010</Year><Month>08</Month><Day>05</Day></DateCreated><DateCompleted><Year>2010</Year><Month>12</Month><Day>14</Day></DateCompleted><DateRevised><Year>2010</Year><Month>08</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1347-5215</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>8</Issue><PubDate><Year>2010</Year></PubDate></JournalIssue><Title>Biological & pharmaceutical bulletin</Title><ISOAbbreviation>Biol. Pharm. 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To characterize their biochemical properties, we performed disaccharide compositional analysis after chondroitinase ABC digestion, high performance size exclusion chromatography (HPSEC) and (1)H-NMR spectroscopy. We also investigated the effects of CSs on fibroblast proliferation and adhesion to determine whether wound healing was accelerated in vitro and proliferation of different mitogen-activated protein kinases (MAPK) signaling pathways was facilitated. The CS purified from sturgeon cartilage was primarily composed of 4-sulfated CS (88.8%) and sturgeon backbone CS contains more than 60% 6-sulfated CS. The average molecular weights of CSs obtained from sturgeon cartilage and backbone were found to be 8 and 43 kDa, respectively. Our results showed that both CSs are able to increase cell adhesion, induce proliferation and migration on fibroblasts and may accelerate wound healing by inducing MAPK signaling pathways.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Im</LastName><ForeName>A-Rang</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Youmie</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yeong Shik</ForeName><Initials>YS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. 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UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20686217</ArticleId><ArticleId IdType="pii">JST.JSTAGE/bpb/33.1268</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><noCountry><name sortKey="Kim, Yeong Shik" sort="Kim, Yeong Shik" uniqKey="Kim Y" first="Yeong Shik" last="Kim">Yeong Shik Kim</name><name sortKey="Park, Youmie" sort="Park, Youmie" uniqKey="Park Y" first="Youmie" last="Park">Youmie Park</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Im, A Rang" sort="Im, A Rang" uniqKey="Im A" first="A-Rang" last="Im">A-Rang Im</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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