Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis.
Identifieur interne : 000341 ( PubMed/Checkpoint ); précédent : 000340; suivant : 000342Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis.
Auteurs : Chuang-Ju Li [République populaire de Chine] ; Fang Gan ; Xi-Hua Chen ; Zhi-Gang Liu ; Luo-Xin Li ; Qi-Wei Wei ; Yong-Kai TangSource :
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [ 1879-1107 ] ; 2011.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Apolipoproteins E (chemistry), Apolipoproteins E (genetics), Cloning, Molecular, Fishes (genetics), Gene Expression Profiling, Molecular Sequence Data, Phylogeny, Protein Conformation, RNA, Messenger (genetics), RNA, Messenger (metabolism), Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Species Specificity.
- MESH :
- chemical , chemistry : Apolipoproteins E.
- chemical , genetics : Apolipoproteins E, RNA, Messenger.
- genetics : Fishes.
- chemical , metabolism : RNA, Messenger.
- Amino Acid Sequence, Animals, Cloning, Molecular, Gene Expression Profiling, Molecular Sequence Data, Phylogeny, Protein Conformation, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Species Specificity.
Abstract
Apolipoproteins are carrier proteins that bind to lipids to form lipoprotein particles and have been shown to play an important role in lipid metabolism. In this study, a full-length cDNA for apolipoprotein E, named AsapoE, was cloned from the Chinese sturgeon (Acipenser sinensis). This cDNA sequence is 1289 bp in length, and codes for a polypeptide of 274 amino acid residues, which is 45% and 42% identical to that of the rainbow trout and zebrafish, respectively, and 39%, 30%, and 29% identical to frog, mouse, and human respectively. The predicted AsApoE protein has a conserved amphipathic α-helix region with the potential to bind to lipids. RT-PCR analysis reveals that AsapoE is expressed in all tissues examined with a preferential expression in the kidney and liver. During the embryo development stage, AsapoE mRNA is low but still detectable at gastrula stage embryos; then AsapoE mRNAs reach a higher level in muscle contraction stage embryos, this relatively stable expression persists during the following embryogenic stages and declines 1 day after hatching. These results will serve as a basis for comparative studies on vertebrate apoE genes.
DOI: 10.1016/j.cbpb.2010.09.008
PubMed: 20868764
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:20868764Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis.</title><author><name sortKey="Li, Chuang Ju" sort="Li, Chuang Ju" uniqKey="Li C" first="Chuang-Ju" last="Li">Chuang-Ju Li</name><affiliation wicri:level="1"><nlm:affiliation>Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081</wicri:regionArea><wicri:noRegion>Wuxi 214081</wicri:noRegion></affiliation></author><author><name sortKey="Gan, Fang" sort="Gan, Fang" uniqKey="Gan F" first="Fang" last="Gan">Fang Gan</name></author><author><name sortKey="Chen, Xi Hua" sort="Chen, Xi Hua" uniqKey="Chen X" first="Xi-Hua" last="Chen">Xi-Hua Chen</name></author><author><name sortKey="Liu, Zhi Gang" sort="Liu, Zhi Gang" uniqKey="Liu Z" first="Zhi-Gang" last="Liu">Zhi-Gang Liu</name></author><author><name sortKey="Li, Luo Xin" sort="Li, Luo Xin" uniqKey="Li L" first="Luo-Xin" last="Li">Luo-Xin Li</name></author><author><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name></author><author><name sortKey="Tang, Yong Kai" sort="Tang, Yong Kai" uniqKey="Tang Y" first="Yong-Kai" last="Tang">Yong-Kai Tang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20868764</idno><idno type="pmid">20868764</idno><idno type="doi">10.1016/j.cbpb.2010.09.008</idno><idno type="wicri:Area/PubMed/Corpus">000367</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000367</idno><idno type="wicri:Area/PubMed/Curation">000367</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000367</idno><idno type="wicri:Area/PubMed/Checkpoint">000367</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000367</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis.</title><author><name sortKey="Li, Chuang Ju" sort="Li, Chuang Ju" uniqKey="Li C" first="Chuang-Ju" last="Li">Chuang-Ju Li</name><affiliation wicri:level="1"><nlm:affiliation>Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081</wicri:regionArea><wicri:noRegion>Wuxi 214081</wicri:noRegion></affiliation></author><author><name sortKey="Gan, Fang" sort="Gan, Fang" uniqKey="Gan F" first="Fang" last="Gan">Fang Gan</name></author><author><name sortKey="Chen, Xi Hua" sort="Chen, Xi Hua" uniqKey="Chen X" first="Xi-Hua" last="Chen">Xi-Hua Chen</name></author><author><name sortKey="Liu, Zhi Gang" sort="Liu, Zhi Gang" uniqKey="Liu Z" first="Zhi-Gang" last="Liu">Zhi-Gang Liu</name></author><author><name sortKey="Li, Luo Xin" sort="Li, Luo Xin" uniqKey="Li L" first="Luo-Xin" last="Li">Luo-Xin Li</name></author><author><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name></author><author><name sortKey="Tang, Yong Kai" sort="Tang, Yong Kai" uniqKey="Tang Y" first="Yong-Kai" last="Tang">Yong-Kai Tang</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology</title><idno type="eISSN">1879-1107</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Apolipoproteins E (chemistry)</term><term>Apolipoproteins E (genetics)</term><term>Cloning, Molecular</term><term>Fishes (genetics)</term><term>Gene Expression Profiling</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Conformation</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Species Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Apolipoproteins E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Apolipoproteins E</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>Gene Expression Profiling</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Conformation</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Apolipoproteins are carrier proteins that bind to lipids to form lipoprotein particles and have been shown to play an important role in lipid metabolism. In this study, a full-length cDNA for apolipoprotein E, named AsapoE, was cloned from the Chinese sturgeon (Acipenser sinensis). This cDNA sequence is 1289 bp in length, and codes for a polypeptide of 274 amino acid residues, which is 45% and 42% identical to that of the rainbow trout and zebrafish, respectively, and 39%, 30%, and 29% identical to frog, mouse, and human respectively. The predicted AsApoE protein has a conserved amphipathic α-helix region with the potential to bind to lipids. RT-PCR analysis reveals that AsapoE is expressed in all tissues examined with a preferential expression in the kidney and liver. During the embryo development stage, AsapoE mRNA is low but still detectable at gastrula stage embryos; then AsapoE mRNAs reach a higher level in muscle contraction stage embryos, this relatively stable expression persists during the following embryogenic stages and declines 1 day after hatching. These results will serve as a basis for comparative studies on vertebrate apoE genes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20868764</PMID><DateCreated><Year>2010</Year><Month>11</Month><Day>30</Day></DateCreated><DateCompleted><Year>2011</Year><Month>09</Month><Day>12</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1107</ISSN><JournalIssue CitedMedium="Internet"><Volume>158</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology</Title><ISOAbbreviation>Comp. Biochem. Physiol. B, Biochem. Mol. Biol.</ISOAbbreviation></Journal><ArticleTitle>Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis.</ArticleTitle><Pagination><MedlinePgn>64-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cbpb.2010.09.008</ELocationID><Abstract><AbstractText>Apolipoproteins are carrier proteins that bind to lipids to form lipoprotein particles and have been shown to play an important role in lipid metabolism. In this study, a full-length cDNA for apolipoprotein E, named AsapoE, was cloned from the Chinese sturgeon (Acipenser sinensis). This cDNA sequence is 1289 bp in length, and codes for a polypeptide of 274 amino acid residues, which is 45% and 42% identical to that of the rainbow trout and zebrafish, respectively, and 39%, 30%, and 29% identical to frog, mouse, and human respectively. The predicted AsApoE protein has a conserved amphipathic α-helix region with the potential to bind to lipids. RT-PCR analysis reveals that AsapoE is expressed in all tissues examined with a preferential expression in the kidney and liver. During the embryo development stage, AsapoE mRNA is low but still detectable at gastrula stage embryos; then AsapoE mRNAs reach a higher level in muscle contraction stage embryos, this relatively stable expression persists during the following embryogenic stages and declines 1 day after hatching. These results will serve as a basis for comparative studies on vertebrate apoE genes.</AbstractText><CopyrightInformation>Copyright © 2010 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chuang-Ju</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gan</LastName><ForeName>Fang</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xi-Hua</ForeName><Initials>XH</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Zhi-Gang</ForeName><Initials>ZG</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Luo-Xin</ForeName><Initials>LX</Initials></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Qi-Wei</ForeName><Initials>QW</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Yong-Kai</ForeName><Initials>YK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>09</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Comp Biochem Physiol B Biochem Mol Biol</MedlineTA><NlmUniqueID>9516061</NlmUniqueID><ISSNLinking>1096-4959</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001057">Apolipoproteins E</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001057" MajorTopicYN="N">Apolipoproteins E</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="Y">Fishes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>07</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>09</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>9</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>9</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>9</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20868764</ArticleId><ArticleId IdType="pii">S1096-4959(10)00229-0</ArticleId><ArticleId IdType="doi">10.1016/j.cbpb.2010.09.008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Xi Hua" sort="Chen, Xi Hua" uniqKey="Chen X" first="Xi-Hua" last="Chen">Xi-Hua Chen</name><name sortKey="Gan, Fang" sort="Gan, Fang" uniqKey="Gan F" first="Fang" last="Gan">Fang Gan</name><name sortKey="Li, Luo Xin" sort="Li, Luo Xin" uniqKey="Li L" first="Luo-Xin" last="Li">Luo-Xin Li</name><name sortKey="Liu, Zhi Gang" sort="Liu, Zhi Gang" uniqKey="Liu Z" first="Zhi-Gang" last="Liu">Zhi-Gang Liu</name><name sortKey="Tang, Yong Kai" sort="Tang, Yong Kai" uniqKey="Tang Y" first="Yong-Kai" last="Tang">Yong-Kai Tang</name><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Li, Chuang Ju" sort="Li, Chuang Ju" uniqKey="Li C" first="Chuang-Ju" last="Li">Chuang-Ju Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000341 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000341 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:20868764
|texte= Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:20868764" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |