The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.
Identifieur interne : 000325 ( PubMed/Checkpoint ); précédent : 000324; suivant : 000326The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.
Auteurs : Arash Akbarzadeh [Iran] ; Hamid Farahmand ; Frouzandeh Mahjoubi ; Mohammad Ali Nematollahi ; Piia Leskinen ; Kalle Rytkönen ; Mikko NikinmaaSource :
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [ 1879-1107 ] ; 2011.
English descriptors
- KwdEn :
- Animals, Ascorbic Acid (biosynthesis), Fishes (embryology), Fishes (genetics), Fishes (growth & development), L-Gulonolactone Oxidase (biosynthesis), L-Gulonolactone Oxidase (genetics), Polymerase Chain Reaction (standards), RNA Stability, RNA, Messenger (metabolism), Reference Standards, Transcription, Genetic.
- MESH :
- chemical , biosynthesis : Ascorbic Acid, L-Gulonolactone Oxidase.
- embryology : Fishes.
- genetics : Fishes, L-Gulonolactone Oxidase.
- growth & development : Fishes.
- chemical , metabolism : RNA, Messenger.
- standards : Polymerase Chain Reaction.
- Animals, RNA Stability, Reference Standards, Transcription, Genetic.
Abstract
l-Gulono-gamma-lactone oxidase (GULO) is a key enzyme for the biosynthesis of ascorbate, which is essential for several cellular functions. In the present study, mRNA expression of GULO gene was evaluated during the early development of Persian sturgeon. First, because there are no comparative studies that have established suitable quantitative real-time PCR reference genes in sturgeons for any physiological conditions, we evaluated six candidate reference genes (ACTB, RPL13, UBQ, RPL6, GAPDH and EF1A) during the early development of Persian sturgeon. The most stable mRNA expression was obtained with RPL6 and ACTB, whereas the least stable was RPL13. After normalization using RPL6, ACTB and RPL6/ACTB combination, the mRNA expression of GULO was highest at the embryonic stage (2days before hatching; P<0.05) and started to decline from hatching of larvae to the rest of the developmental time-points. This suggests that the vitamin C requirements are highest during early life stages, and it is likely that the changes in GULO mRNA expression are associated with changes in GULO enzyme activity.
DOI: 10.1016/j.cbpb.2010.12.005
PubMed: 21199677
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:21199677Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.</title><author><name sortKey="Akbarzadeh, Arash" sort="Akbarzadeh, Arash" uniqKey="Akbarzadeh A" first="Arash" last="Akbarzadeh">Arash Akbarzadeh</name><affiliation wicri:level="1"><nlm:affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. akbarzadeh@ut.ac.ir</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj</wicri:regionArea><wicri:noRegion>Karaj</wicri:noRegion></affiliation></author><author><name sortKey="Farahmand, Hamid" sort="Farahmand, Hamid" uniqKey="Farahmand H" first="Hamid" last="Farahmand">Hamid Farahmand</name></author><author><name sortKey="Mahjoubi, Frouzandeh" sort="Mahjoubi, Frouzandeh" uniqKey="Mahjoubi F" first="Frouzandeh" last="Mahjoubi">Frouzandeh Mahjoubi</name></author><author><name sortKey="Nematollahi, Mohammad Ali" sort="Nematollahi, Mohammad Ali" uniqKey="Nematollahi M" first="Mohammad Ali" last="Nematollahi">Mohammad Ali Nematollahi</name></author><author><name sortKey="Leskinen, Piia" sort="Leskinen, Piia" uniqKey="Leskinen P" first="Piia" last="Leskinen">Piia Leskinen</name></author><author><name sortKey="Rytkonen, Kalle" sort="Rytkonen, Kalle" uniqKey="Rytkonen K" first="Kalle" last="Rytkönen">Kalle Rytkönen</name></author><author><name sortKey="Nikinmaa, Mikko" sort="Nikinmaa, Mikko" uniqKey="Nikinmaa M" first="Mikko" last="Nikinmaa">Mikko Nikinmaa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21199677</idno><idno type="pmid">21199677</idno><idno type="doi">10.1016/j.cbpb.2010.12.005</idno><idno type="wicri:Area/PubMed/Corpus">000358</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000358</idno><idno type="wicri:Area/PubMed/Curation">000358</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000358</idno><idno type="wicri:Area/PubMed/Checkpoint">000358</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000358</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.</title><author><name sortKey="Akbarzadeh, Arash" sort="Akbarzadeh, Arash" uniqKey="Akbarzadeh A" first="Arash" last="Akbarzadeh">Arash Akbarzadeh</name><affiliation wicri:level="1"><nlm:affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. akbarzadeh@ut.ac.ir</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj</wicri:regionArea><wicri:noRegion>Karaj</wicri:noRegion></affiliation></author><author><name sortKey="Farahmand, Hamid" sort="Farahmand, Hamid" uniqKey="Farahmand H" first="Hamid" last="Farahmand">Hamid Farahmand</name></author><author><name sortKey="Mahjoubi, Frouzandeh" sort="Mahjoubi, Frouzandeh" uniqKey="Mahjoubi F" first="Frouzandeh" last="Mahjoubi">Frouzandeh Mahjoubi</name></author><author><name sortKey="Nematollahi, Mohammad Ali" sort="Nematollahi, Mohammad Ali" uniqKey="Nematollahi M" first="Mohammad Ali" last="Nematollahi">Mohammad Ali Nematollahi</name></author><author><name sortKey="Leskinen, Piia" sort="Leskinen, Piia" uniqKey="Leskinen P" first="Piia" last="Leskinen">Piia Leskinen</name></author><author><name sortKey="Rytkonen, Kalle" sort="Rytkonen, Kalle" uniqKey="Rytkonen K" first="Kalle" last="Rytkönen">Kalle Rytkönen</name></author><author><name sortKey="Nikinmaa, Mikko" sort="Nikinmaa, Mikko" uniqKey="Nikinmaa M" first="Mikko" last="Nikinmaa">Mikko Nikinmaa</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology</title><idno type="eISSN">1879-1107</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Ascorbic Acid (biosynthesis)</term><term>Fishes (embryology)</term><term>Fishes (genetics)</term><term>Fishes (growth & development)</term><term>L-Gulonolactone Oxidase (biosynthesis)</term><term>L-Gulonolactone Oxidase (genetics)</term><term>Polymerase Chain Reaction (standards)</term><term>RNA Stability</term><term>RNA, Messenger (metabolism)</term><term>Reference Standards</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Ascorbic Acid</term><term>L-Gulonolactone Oxidase</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term><term>L-Gulonolactone Oxidase</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>RNA Stability</term><term>Reference Standards</term><term>Transcription, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">l-Gulono-gamma-lactone oxidase (GULO) is a key enzyme for the biosynthesis of ascorbate, which is essential for several cellular functions. In the present study, mRNA expression of GULO gene was evaluated during the early development of Persian sturgeon. First, because there are no comparative studies that have established suitable quantitative real-time PCR reference genes in sturgeons for any physiological conditions, we evaluated six candidate reference genes (ACTB, RPL13, UBQ, RPL6, GAPDH and EF1A) during the early development of Persian sturgeon. The most stable mRNA expression was obtained with RPL6 and ACTB, whereas the least stable was RPL13. After normalization using RPL6, ACTB and RPL6/ACTB combination, the mRNA expression of GULO was highest at the embryonic stage (2days before hatching; P<0.05) and started to decline from hatching of larvae to the rest of the developmental time-points. This suggests that the vitamin C requirements are highest during early life stages, and it is likely that the changes in GULO mRNA expression are associated with changes in GULO enzyme activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21199677</PMID><DateCreated><Year>2011</Year><Month>02</Month><Day>23</Day></DateCreated><DateCompleted><Year>2011</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1107</ISSN><JournalIssue CitedMedium="Internet"><Volume>158</Volume><Issue>4</Issue><PubDate><Year>2011</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology</Title><ISOAbbreviation>Comp. Biochem. Physiol. B, Biochem. Mol. Biol.</ISOAbbreviation></Journal><ArticleTitle>The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.</ArticleTitle><Pagination><MedlinePgn>282-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cbpb.2010.12.005</ELocationID><Abstract><AbstractText>l-Gulono-gamma-lactone oxidase (GULO) is a key enzyme for the biosynthesis of ascorbate, which is essential for several cellular functions. In the present study, mRNA expression of GULO gene was evaluated during the early development of Persian sturgeon. First, because there are no comparative studies that have established suitable quantitative real-time PCR reference genes in sturgeons for any physiological conditions, we evaluated six candidate reference genes (ACTB, RPL13, UBQ, RPL6, GAPDH and EF1A) during the early development of Persian sturgeon. The most stable mRNA expression was obtained with RPL6 and ACTB, whereas the least stable was RPL13. After normalization using RPL6, ACTB and RPL6/ACTB combination, the mRNA expression of GULO was highest at the embryonic stage (2days before hatching; P<0.05) and started to decline from hatching of larvae to the rest of the developmental time-points. This suggests that the vitamin C requirements are highest during early life stages, and it is likely that the changes in GULO mRNA expression are associated with changes in GULO enzyme activity.</AbstractText><CopyrightInformation>Copyright © 2010 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Akbarzadeh</LastName><ForeName>Arash</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. akbarzadeh@ut.ac.ir</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Farahmand</LastName><ForeName>Hamid</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Mahjoubi</LastName><ForeName>Frouzandeh</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Nematollahi</LastName><ForeName>Mohammad Ali</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Leskinen</LastName><ForeName>Piia</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Rytkönen</LastName><ForeName>Kalle</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Nikinmaa</LastName><ForeName>Mikko</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>01</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Comp Biochem Physiol B Biochem Mol Biol</MedlineTA><NlmUniqueID>9516061</NlmUniqueID><ISSNLinking>1096-4959</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.3.8</RegistryNumber><NameOfSubstance UI="D050544">L-Gulonolactone Oxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>PQ6CK8PD0R</RegistryNumber><NameOfSubstance UI="D001205">Ascorbic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001205" MajorTopicYN="N">Ascorbic Acid</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000196" MajorTopicYN="N">embryology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050544" MajorTopicYN="N">L-Gulonolactone Oxidase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000592" MajorTopicYN="N">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020871" MajorTopicYN="N">RNA Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012015" MajorTopicYN="N">Reference Standards</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>09</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>12</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>12</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21199677</ArticleId><ArticleId IdType="pii">S1096-4959(10)00295-2</ArticleId><ArticleId IdType="doi">10.1016/j.cbpb.2010.12.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Iran</li></country></list><tree><noCountry><name sortKey="Farahmand, Hamid" sort="Farahmand, Hamid" uniqKey="Farahmand H" first="Hamid" last="Farahmand">Hamid Farahmand</name><name sortKey="Leskinen, Piia" sort="Leskinen, Piia" uniqKey="Leskinen P" first="Piia" last="Leskinen">Piia Leskinen</name><name sortKey="Mahjoubi, Frouzandeh" sort="Mahjoubi, Frouzandeh" uniqKey="Mahjoubi F" first="Frouzandeh" last="Mahjoubi">Frouzandeh Mahjoubi</name><name sortKey="Nematollahi, Mohammad Ali" sort="Nematollahi, Mohammad Ali" uniqKey="Nematollahi M" first="Mohammad Ali" last="Nematollahi">Mohammad Ali Nematollahi</name><name sortKey="Nikinmaa, Mikko" sort="Nikinmaa, Mikko" uniqKey="Nikinmaa M" first="Mikko" last="Nikinmaa">Mikko Nikinmaa</name><name sortKey="Rytkonen, Kalle" sort="Rytkonen, Kalle" uniqKey="Rytkonen K" first="Kalle" last="Rytkönen">Kalle Rytkönen</name></noCountry><country name="Iran"><noRegion><name sortKey="Akbarzadeh, Arash" sort="Akbarzadeh, Arash" uniqKey="Akbarzadeh A" first="Arash" last="Akbarzadeh">Arash Akbarzadeh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000325 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000325 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:21199677
|texte= The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:21199677" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |