Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii.
Identifieur interne : 000299 ( PubMed/Checkpoint ); précédent : 000298; suivant : 000300Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii.
Auteurs : Julio Berbejillo [Uruguay] ; Anabel Martinez-Bengochea ; Gabriela Bedo ; Frédéric Brunet ; Jean-Nicolas Volff ; Denise Vizziano-CantonnetSource :
- Molecular reproduction and development [ 1098-2795 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Cell Differentiation (physiology), Fish Proteins (biosynthesis), Fishes, Gene Expression Regulation (physiology), Insulin-Like Growth Factor I (biosynthesis), Male, Phosphoproteins (biosynthesis), Phylogeny, Sex Differentiation (physiology), Steroid 17-alpha-Hydroxylase (biosynthesis), Testis (cytology), Testis (growth & development), Transcription Factors (biosynthesis), Transcriptome.
- MESH :
- chemical , biosynthesis : Fish Proteins, Insulin-Like Growth Factor I, Phosphoproteins, Steroid 17-alpha-Hydroxylase, Transcription Factors.
- cytology : Testis.
- growth & development : Testis.
- physiology : Cell Differentiation, Gene Expression Regulation, Sex Differentiation.
- Animals, Fishes, Male, Phylogeny, Transcriptome.
Abstract
The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii, by expression profiling of Sertoli cell transcription factors (dmrt1, sox9) that control testis differentiation in vertebrates; Leydig cell factors (cyp17a1, star) affecting androgen production; the androgen receptor (ar); a growth factor controlling testis development (igf1); and a gene coding for a gonadotropin hormone (lh). Two genes were characterised for the first time in the Siberian sturgeon (dmrt1, cyp17a1), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex-dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre- and post-differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post-differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9. The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors (dmrt1, sox9) and of genes involved in the production and receptivity of androgens (cyp17a1, star and ar) together with lh. Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.
DOI: 10.1002/mrd.22053
PubMed: 22618959
Affiliations:
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Uruguay, Montevideo, Uruguay.</nlm:affiliation><country xml:lang="fr">Uruguay</country><wicri:regionArea>Laboratorio de Fisiología de la Reproducción y Ecología de Peces, Instituto de Biología, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Montevideo</wicri:regionArea><wicri:noRegion>Montevideo</wicri:noRegion></affiliation></author><author><name sortKey="Martinez Bengochea, Anabel" sort="Martinez Bengochea, Anabel" uniqKey="Martinez Bengochea A" first="Anabel" last="Martinez-Bengochea">Anabel Martinez-Bengochea</name></author><author><name sortKey="Bedo, Gabriela" sort="Bedo, Gabriela" uniqKey="Bedo G" first="Gabriela" last="Bedo">Gabriela Bedo</name></author><author><name sortKey="Brunet, Frederic" sort="Brunet, Frederic" uniqKey="Brunet F" first="Frédéric" last="Brunet">Frédéric Brunet</name></author><author><name sortKey="Volff, Jean Nicolas" sort="Volff, Jean Nicolas" uniqKey="Volff J" first="Jean-Nicolas" last="Volff">Jean-Nicolas Volff</name></author><author><name sortKey="Vizziano Cantonnet, Denise" sort="Vizziano Cantonnet, Denise" uniqKey="Vizziano Cantonnet D" first="Denise" last="Vizziano-Cantonnet">Denise Vizziano-Cantonnet</name></author></analytic><series><title level="j">Molecular reproduction and development</title><idno type="eISSN">1098-2795</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Differentiation (physiology)</term><term>Fish Proteins (biosynthesis)</term><term>Fishes</term><term>Gene Expression Regulation (physiology)</term><term>Insulin-Like Growth Factor I (biosynthesis)</term><term>Male</term><term>Phosphoproteins (biosynthesis)</term><term>Phylogeny</term><term>Sex Differentiation (physiology)</term><term>Steroid 17-alpha-Hydroxylase (biosynthesis)</term><term>Testis (cytology)</term><term>Testis (growth & development)</term><term>Transcription Factors (biosynthesis)</term><term>Transcriptome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Fish Proteins</term><term>Insulin-Like Growth Factor I</term><term>Phosphoproteins</term><term>Steroid 17-alpha-Hydroxylase</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Testis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Testis</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Differentiation</term><term>Gene Expression Regulation</term><term>Sex Differentiation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fishes</term><term>Male</term><term>Phylogeny</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii, by expression profiling of Sertoli cell transcription factors (dmrt1, sox9) that control testis differentiation in vertebrates; Leydig cell factors (cyp17a1, star) affecting androgen production; the androgen receptor (ar); a growth factor controlling testis development (igf1); and a gene coding for a gonadotropin hormone (lh). Two genes were characterised for the first time in the Siberian sturgeon (dmrt1, cyp17a1), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex-dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre- and post-differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post-differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9. The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors (dmrt1, sox9) and of genes involved in the production and receptivity of androgens (cyp17a1, star and ar) together with lh. Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22618959</PMID><DateCreated><Year>2012</Year><Month>08</Month><Day>13</Day></DateCreated><DateCompleted><Year>2012</Year><Month>12</Month><Day>17</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-2795</ISSN><JournalIssue CitedMedium="Internet"><Volume>79</Volume><Issue>8</Issue><PubDate><Year>2012</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Molecular reproduction and development</Title><ISOAbbreviation>Mol. Reprod. 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Two genes were characterised for the first time in the Siberian sturgeon (dmrt1, cyp17a1), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex-dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre- and post-differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post-differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9. The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors (dmrt1, sox9) and of genes involved in the production and receptivity of androgens (cyp17a1, star and ar) together with lh. Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.</AbstractText><CopyrightInformation>Copyright © 2012 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berbejillo</LastName><ForeName>Julio</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratorio de Fisiología de la Reproducción y Ecología de Peces, Instituto de Biología, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Montevideo, Uruguay.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martinez-Bengochea</LastName><ForeName>Anabel</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bedo</LastName><ForeName>Gabriela</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Brunet</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Volff</LastName><ForeName>Jean-Nicolas</ForeName><Initials>JN</Initials></Author><Author ValidYN="Y"><LastName>Vizziano-Cantonnet</LastName><ForeName>Denise</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>07</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Reprod Dev</MedlineTA><NlmUniqueID>8903333</NlmUniqueID><ISSNLinking>1040-452X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C110547">DMRT1 protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029941">Fish 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MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>12</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>05</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22618959</ArticleId><ArticleId IdType="doi">10.1002/mrd.22053</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Uruguay</li></country></list><tree><noCountry><name sortKey="Bedo, Gabriela" sort="Bedo, Gabriela" uniqKey="Bedo G" first="Gabriela" last="Bedo">Gabriela Bedo</name><name sortKey="Brunet, Frederic" sort="Brunet, Frederic" uniqKey="Brunet F" first="Frédéric" last="Brunet">Frédéric Brunet</name><name sortKey="Martinez Bengochea, Anabel" sort="Martinez Bengochea, Anabel" uniqKey="Martinez Bengochea A" first="Anabel" last="Martinez-Bengochea">Anabel Martinez-Bengochea</name><name sortKey="Vizziano Cantonnet, Denise" sort="Vizziano Cantonnet, Denise" uniqKey="Vizziano Cantonnet D" first="Denise" last="Vizziano-Cantonnet">Denise Vizziano-Cantonnet</name><name sortKey="Volff, Jean Nicolas" sort="Volff, Jean Nicolas" uniqKey="Volff J" first="Jean-Nicolas" last="Volff">Jean-Nicolas Volff</name></noCountry><country name="Uruguay"><noRegion><name sortKey="Berbejillo, Julio" sort="Berbejillo, Julio" uniqKey="Berbejillo J" first="Julio" last="Berbejillo">Julio Berbejillo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce 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