Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17β-estradiol on gene expression.
Identifieur interne : 000239 ( PubMed/Checkpoint ); précédent : 000238; suivant : 000240Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17β-estradiol on gene expression.
Auteurs : Huamei Yue [République populaire de Chine] ; Huan Ye ; Xihua Chen ; Hong Cao ; Chuangju LiSource :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [ 1531-4332 ] ; 2013.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Estradiol, Fishes (genetics), Gene Expression, Gonadotropin-Releasing Hormone (biosynthesis), Gonadotropin-Releasing Hormone (chemistry), Gonadotropin-Releasing Hormone (genetics), Humans, Molecular Sequence Data, Protein Precursors (chemistry), Sequence Alignment, Sequence Analysis, DNA, Tissue Distribution.
- MESH :
- chemical , biosynthesis : Gonadotropin-Releasing Hormone.
- chemical , chemistry : Gonadotropin-Releasing Hormone, Protein Precursors.
- chemical , genetics : Gonadotropin-Releasing Hormone.
- chemical : Estradiol.
- genetics : Fishes.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Gene Expression, Humans, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Tissue Distribution.
Abstract
The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino acids, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17β-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P<0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon.
DOI: 10.1016/j.cbpa.2013.08.011
PubMed: 23994573
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17β-estradiol on gene expression.</title><author><name sortKey="Yue, Huamei" sort="Yue, Huamei" uniqKey="Yue H" first="Huamei" last="Yue">Huamei Yue</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081</wicri:regionArea><wicri:noRegion>Wuxi 214081</wicri:noRegion></affiliation></author><author><name sortKey="Ye, Huan" sort="Ye, Huan" uniqKey="Ye H" first="Huan" last="Ye">Huan Ye</name></author><author><name sortKey="Chen, Xihua" sort="Chen, Xihua" uniqKey="Chen X" first="Xihua" last="Chen">Xihua Chen</name></author><author><name sortKey="Cao, Hong" sort="Cao, Hong" uniqKey="Cao H" first="Hong" last="Cao">Hong Cao</name></author><author><name sortKey="Li, Chuangju" sort="Li, Chuangju" uniqKey="Li C" first="Chuangju" last="Li">Chuangju Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23994573</idno><idno type="pmid">23994573</idno><idno type="doi">10.1016/j.cbpa.2013.08.011</idno><idno type="wicri:Area/PubMed/Corpus">000226</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000226</idno><idno type="wicri:Area/PubMed/Curation">000226</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000226</idno><idno type="wicri:Area/PubMed/Checkpoint">000226</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000226</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17β-estradiol on gene expression.</title><author><name sortKey="Yue, Huamei" sort="Yue, Huamei" uniqKey="Yue H" first="Huamei" last="Yue">Huamei Yue</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081</wicri:regionArea><wicri:noRegion>Wuxi 214081</wicri:noRegion></affiliation></author><author><name sortKey="Ye, Huan" sort="Ye, Huan" uniqKey="Ye H" first="Huan" last="Ye">Huan Ye</name></author><author><name sortKey="Chen, Xihua" sort="Chen, Xihua" uniqKey="Chen X" first="Xihua" last="Chen">Xihua Chen</name></author><author><name sortKey="Cao, Hong" sort="Cao, Hong" uniqKey="Cao H" first="Hong" last="Cao">Hong Cao</name></author><author><name sortKey="Li, Chuangju" sort="Li, Chuangju" uniqKey="Li C" first="Chuangju" last="Li">Chuangju Li</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</title><idno type="eISSN">1531-4332</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Estradiol</term><term>Fishes (genetics)</term><term>Gene Expression</term><term>Gonadotropin-Releasing Hormone (biosynthesis)</term><term>Gonadotropin-Releasing Hormone (chemistry)</term><term>Gonadotropin-Releasing Hormone (genetics)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Protein Precursors (chemistry)</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Tissue Distribution</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Gonadotropin-Releasing Hormone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gonadotropin-Releasing Hormone</term><term>Protein Precursors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Gonadotropin-Releasing Hormone</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Estradiol</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Gene Expression</term><term>Humans</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Tissue Distribution</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino acids, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17β-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P<0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23994573</PMID><DateCreated><Year>2013</Year><Month>10</Month><Day>28</Day></DateCreated><DateCompleted><Year>2014</Year><Month>05</Month><Day>28</Day></DateCompleted><DateRevised><Year>2013</Year><Month>10</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1531-4332</ISSN><JournalIssue CitedMedium="Internet"><Volume>166</Volume><Issue>4</Issue><PubDate><Year>2013</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</Title><ISOAbbreviation>Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17β-estradiol on gene expression.</ArticleTitle><Pagination><MedlinePgn>529-37</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cbpa.2013.08.011</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1095-6433(13)00224-9</ELocationID><Abstract><AbstractText>The Chinese sturgeon, Acipenser sinensis, is a rare and large-sized anadromous bony fish and understanding of its reproductive regulation is a precondition for controlled reproduction. In this study, two gonadotropin-releasing hormone (GnRH) precursor cDNAs, AsGnRH1 (mammalian type) and AsGnRH2 (chicken type 2), were sequenced in A. sinensis. The precursor cDNAs of the AsGnRH1 and AsGnRH2 are 381 and 649 base pairs (bp), encoding signal peptide plus precursors of 92 and 86 amino acids, respectively. Multiple sequence alignment suggests that AsGnRH1 and AsGnRH2 decapeptides are highly conserved among vertebrates. Besides, AsGnRH1 had closer evolutionary relationship with tetrapods, while AsGnRH2 was conservatively grouped with teleosts in the phylogenetic analysis. Tissue distribution analysis shows that AsGnRH2 is exclusively transcribed in the brain, whereas AsGnRH1 exhibits more extensive tissue distribution including brain, liver, spleen and gonad. Furthermore, Chinese sturgeons were subcutaneously implanted with 17β-estradiol (E2) and the effect of E2 on brain GnRH mRNA levels was evaluated by real-time PCR. A significant increase in AsGnRH1 and AsGnRH2 mRNA levels is detected in fish receiving E2 implantation compared to controls after one month (P<0.05). These results indicate that E2 exerts positive feedback effects on the transcription of the two GnRHs in immature Chinese sturgeon.</AbstractText><CopyrightInformation>© 2013.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yue</LastName><ForeName>Huamei</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi 214081, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Huan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xihua</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chuangju</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>KC529342</AccessionNumber><AccessionNumber>KC529343</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>08</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Comp Biochem Physiol A Mol Integr Physiol</MedlineTA><NlmUniqueID>9806096</NlmUniqueID><ISSNLinking>1095-6433</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011498">Protein Precursors</NameOfSubstance></Chemical><Chemical><RegistryNumber>33515-09-2</RegistryNumber><NameOfSubstance UI="D007987">Gonadotropin-Releasing Hormone</NameOfSubstance></Chemical><Chemical><RegistryNumber>4TI98Z838E</RegistryNumber><NameOfSubstance UI="D004958">Estradiol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Comp Biochem Physiol A Mol Integr Physiol. 2014 Mar;169:24</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, 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MajorTopicYN="N">Protein Precursors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014018" MajorTopicYN="N">Tissue Distribution</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">17β-estradiol</Keyword><Keyword MajorTopicYN="N">BPG</Keyword><Keyword MajorTopicYN="N">Chinese sturgeon</Keyword><Keyword MajorTopicYN="N">E2</Keyword><Keyword MajorTopicYN="N">GAP</Keyword><Keyword MajorTopicYN="N">GKR</Keyword><Keyword MajorTopicYN="N">GnRH</Keyword><Keyword MajorTopicYN="N">GnRH associated peptide</Keyword><Keyword MajorTopicYN="N">GnRH1</Keyword><Keyword MajorTopicYN="N">LS</Keyword><Keyword MajorTopicYN="N">ORF</Keyword><Keyword 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PubStatus="accepted"><Year>2013</Year><Month>08</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>5</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23994573</ArticleId><ArticleId IdType="pii">S1095-6433(13)00224-9</ArticleId><ArticleId IdType="doi">10.1016/j.cbpa.2013.08.011</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Cao, Hong" sort="Cao, Hong" uniqKey="Cao H" first="Hong" last="Cao">Hong Cao</name><name sortKey="Chen, Xihua" sort="Chen, Xihua" uniqKey="Chen X" first="Xihua" last="Chen">Xihua Chen</name><name sortKey="Li, Chuangju" sort="Li, Chuangju" uniqKey="Li C" first="Chuangju" last="Li">Chuangju Li</name><name sortKey="Ye, Huan" sort="Ye, Huan" uniqKey="Ye H" first="Huan" last="Ye">Huan Ye</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yue, Huamei" sort="Yue, Huamei" uniqKey="Yue H" first="Huamei" last="Yue">Huamei Yue</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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