New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen.
Identifieur interne : 000118 ( PubMed/Checkpoint ); précédent : 000117; suivant : 000119New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen.
Auteurs : S. Judycka [Pologne] ; M. Szczepkowski [Pologne] ; A. Ciereszko [Pologne] ; G J Dietrich [Pologne]Source :
- Cryobiology [ 1090-2392 ] ; 2015.
English descriptors
- KwdEn :
- Animals, Cryopreservation (methods), Cryoprotective Agents (pharmacology), Fertilization (physiology), Fishes, Freezing (adverse effects), Glucose (pharmacology), Male, Methanol (pharmacology), Semen (physiology), Semen Analysis, Semen Preservation (methods), Sperm Motility (drug effects), Sperm Motility (physiology), Spermatozoa (drug effects).
- MESH :
- chemical , pharmacology : Cryoprotective Agents, Glucose, Methanol.
- adverse effects : Freezing.
- drug effects : Sperm Motility, Spermatozoa.
- methods : Cryopreservation, Semen Preservation.
- physiology : Fertilization, Semen, Sperm Motility.
- Animals, Fishes, Male, Semen Analysis.
Abstract
The goal of this study was to develop a simple glucose-methanol extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen. Semen quality was assessed by determining post-thaw sperm motility and fertilizing ability at hatching stage. We tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in a methanol extender on post-thaw sperm motility. Sperm motility parameters and fertilizing ability of semen cryopreserved in 0.1 M glucose in 15% methanol (GM) were compared to previously described Tris-sucrose-KCl in 10% - methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 30 min equilibration in GM extender before cryopreservation and 30 min of post-thaw storage were determined. The beneficial effect of the glucose for semen cryopreservation was related to its concentration with a quite narrow optimum of 0.1 to -0.15 M. The fertilization rates of frozen/thawed sperm were similar for both (TSKM and GM) tested extenders. The sperm motility and fertilization rate were not affected either by 30 min equilibration in GM extender or by 30 min of post-thaw storage. Our work indicates that the use a simple extender consisting of 0.1M glucose in 15% methanol can be an alternative cryopreservation method to those previously described for sturgeons. The use of an equilibration period and the possibility of post-thaw semen storage can improve organization of hatchery work and help with logistics of large-scale hatchery operations.
DOI: 10.1016/j.cryobiol.2015.02.005
PubMed: 25725469
Affiliations:
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Judycka</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn</wicri:regionArea><wicri:noRegion>10-747 Olsztyn</wicri:noRegion></affiliation></author><author><name sortKey="Szczepkowski, M" sort="Szczepkowski, M" uniqKey="Szczepkowski M" first="M" last="Szczepkowski">M. Szczepkowski</name><affiliation wicri:level="1"><nlm:affiliation>Department of Sturgeon Fish Breeding Inland Fisheries Institute in Olsztyn, Pieczarki 50, 11-610 Pozezdrze, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Sturgeon Fish Breeding Inland Fisheries Institute in Olsztyn, Pieczarki 50, 11-610 Pozezdrze</wicri:regionArea><wicri:noRegion>11-610 Pozezdrze</wicri:noRegion></affiliation></author><author><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A" last="Ciereszko">A. Ciereszko</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn</wicri:regionArea><wicri:noRegion>10-747 Olsztyn</wicri:noRegion></affiliation></author><author><name sortKey="Dietrich, G J" sort="Dietrich, G J" uniqKey="Dietrich G" first="G J" last="Dietrich">G J Dietrich</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. Electronic address: g.dietrich@pan.olsztyn.pl.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn</wicri:regionArea><wicri:noRegion>10-747 Olsztyn</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cryobiology</title><idno type="eISSN">1090-2392</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cryopreservation (methods)</term><term>Cryoprotective Agents (pharmacology)</term><term>Fertilization (physiology)</term><term>Fishes</term><term>Freezing (adverse effects)</term><term>Glucose (pharmacology)</term><term>Male</term><term>Methanol (pharmacology)</term><term>Semen (physiology)</term><term>Semen Analysis</term><term>Semen Preservation (methods)</term><term>Sperm Motility (drug effects)</term><term>Sperm Motility (physiology)</term><term>Spermatozoa (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cryoprotective Agents</term><term>Glucose</term><term>Methanol</term></keywords><keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Freezing</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Sperm Motility</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term><term>Semen Preservation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fertilization</term><term>Semen</term><term>Sperm Motility</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fishes</term><term>Male</term><term>Semen Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The goal of this study was to develop a simple glucose-methanol extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen. Semen quality was assessed by determining post-thaw sperm motility and fertilizing ability at hatching stage. We tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in a methanol extender on post-thaw sperm motility. Sperm motility parameters and fertilizing ability of semen cryopreserved in 0.1 M glucose in 15% methanol (GM) were compared to previously described Tris-sucrose-KCl in 10% - methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 30 min equilibration in GM extender before cryopreservation and 30 min of post-thaw storage were determined. The beneficial effect of the glucose for semen cryopreservation was related to its concentration with a quite narrow optimum of 0.1 to -0.15 M. The fertilization rates of frozen/thawed sperm were similar for both (TSKM and GM) tested extenders. The sperm motility and fertilization rate were not affected either by 30 min equilibration in GM extender or by 30 min of post-thaw storage. Our work indicates that the use a simple extender consisting of 0.1M glucose in 15% methanol can be an alternative cryopreservation method to those previously described for sturgeons. The use of an equilibration period and the possibility of post-thaw semen storage can improve organization of hatchery work and help with logistics of large-scale hatchery operations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25725469</PMID><DateCreated><Year>2015</Year><Month>03</Month><Day>21</Day></DateCreated><DateCompleted><Year>2016</Year><Month>04</Month><Day>18</Day></DateCompleted><DateRevised><Year>2015</Year><Month>03</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2392</ISSN><JournalIssue CitedMedium="Internet"><Volume>70</Volume><Issue>2</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Cryobiology</Title><ISOAbbreviation>Cryobiology</ISOAbbreviation></Journal><ArticleTitle>New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen.</ArticleTitle><Pagination><MedlinePgn>184-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cryobiol.2015.02.005</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0011-2240(15)00033-4</ELocationID><Abstract><AbstractText>The goal of this study was to develop a simple glucose-methanol extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen. Semen quality was assessed by determining post-thaw sperm motility and fertilizing ability at hatching stage. We tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in a methanol extender on post-thaw sperm motility. Sperm motility parameters and fertilizing ability of semen cryopreserved in 0.1 M glucose in 15% methanol (GM) were compared to previously described Tris-sucrose-KCl in 10% - methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 30 min equilibration in GM extender before cryopreservation and 30 min of post-thaw storage were determined. The beneficial effect of the glucose for semen cryopreservation was related to its concentration with a quite narrow optimum of 0.1 to -0.15 M. The fertilization rates of frozen/thawed sperm were similar for both (TSKM and GM) tested extenders. The sperm motility and fertilization rate were not affected either by 30 min equilibration in GM extender or by 30 min of post-thaw storage. Our work indicates that the use a simple extender consisting of 0.1M glucose in 15% methanol can be an alternative cryopreservation method to those previously described for sturgeons. The use of an equilibration period and the possibility of post-thaw semen storage can improve organization of hatchery work and help with logistics of large-scale hatchery operations.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Judycka</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Szczepkowski</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Sturgeon Fish Breeding Inland Fisheries Institute in Olsztyn, Pieczarki 50, 11-610 Pozezdrze, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ciereszko</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dietrich</LastName><ForeName>G J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. Electronic address: g.dietrich@pan.olsztyn.pl.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>02</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Cryobiology</MedlineTA><NlmUniqueID>0006252</NlmUniqueID><ISSNLinking>0011-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003451">Cryoprotective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical><Chemical><RegistryNumber>Y4S76JWI15</RegistryNumber><NameOfSubstance UI="D000432">Methanol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="N">Cryopreservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003451" MajorTopicYN="N">Cryoprotective Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005306" MajorTopicYN="N">Fertilization</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005615" MajorTopicYN="N">Freezing</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="N">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000432" MajorTopicYN="N">Methanol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012661" MajorTopicYN="N">Semen</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055101" MajorTopicYN="N">Semen Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012662" MajorTopicYN="N">Semen Preservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013081" MajorTopicYN="N">Sperm Motility</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cryopreservation</Keyword><Keyword MajorTopicYN="N">Fertilization ability</Keyword><Keyword MajorTopicYN="N">Siberian sturgeon</Keyword><Keyword MajorTopicYN="N">Sperm motility</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>02</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25725469</ArticleId><ArticleId IdType="pii">S0011-2240(15)00033-4</ArticleId><ArticleId IdType="doi">10.1016/j.cryobiol.2015.02.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Judycka, S" sort="Judycka, S" uniqKey="Judycka S" first="S" last="Judycka">S. Judycka</name></noRegion><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A" last="Ciereszko">A. Ciereszko</name><name sortKey="Dietrich, G J" sort="Dietrich, G J" uniqKey="Dietrich G" first="G J" last="Dietrich">G J Dietrich</name><name sortKey="Szczepkowski, M" sort="Szczepkowski, M" uniqKey="Szczepkowski M" first="M" last="Szczepkowski">M. Szczepkowski</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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