Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads.
Identifieur interne : 000080 ( PubMed/Checkpoint ); précédent : 000079; suivant : 000081Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads.
Auteurs : Yadong Chen ; Yongtao Xia [République populaire de Chine] ; Changwei Shao [République populaire de Chine] ; Lei Han [République populaire de Chine] ; Xuejie Chen ; Mengjun Yu ; Zhenxia Sha [Oman]Source :
- Physiological genomics [ 1531-2267 ] ; 2016.
Abstract
As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated >60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.
DOI: 10.1152/physiolgenomics.00113.2015
PubMed: 27199458
Affiliations:
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Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and.</nlm:affiliation><wicri:noCountry code="subField">China; and</wicri:noCountry></affiliation></author><author><name sortKey="Xia, Yongtao" sort="Xia, Yongtao" uniqKey="Xia Y" first="Yongtao" last="Xia">Yongtao Xia</name><affiliation wicri:level="1"><nlm:affiliation>Hangzhou Qiandaohu Xunlong Sci-Tech Development Company Limited, Quzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou Qiandaohu Xunlong Sci-Tech Development Company Limited, Quzhou</wicri:regionArea><wicri:noRegion>Quzhou</wicri:noRegion></affiliation></author><author><name sortKey="Shao, Changwei" sort="Shao, Changwei" uniqKey="Shao C" first="Changwei" last="Shao">Changwei Shao</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China;</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author><author><name sortKey="Han, Lei" sort="Han, Lei" uniqKey="Han L" first="Lei" last="Han">Lei Han</name><affiliation wicri:level="1"><nlm:affiliation>Hangzhou Qiandaohu Xunlong Sci-Tech Development Company Limited, Quzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou Qiandaohu Xunlong Sci-Tech Development Company Limited, Quzhou</wicri:regionArea><wicri:noRegion>Quzhou</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xuejie" sort="Chen, Xuejie" uniqKey="Chen X" first="Xuejie" last="Chen">Xuejie Chen</name><affiliation><nlm:affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and.</nlm:affiliation><wicri:noCountry code="subField">China; and</wicri:noCountry></affiliation></author><author><name sortKey="Yu, Mengjun" sort="Yu, Mengjun" uniqKey="Yu M" first="Mengjun" last="Yu">Mengjun Yu</name><affiliation><nlm:affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and.</nlm:affiliation><wicri:noCountry code="subField">China; and</wicri:noCountry></affiliation></author><author><name sortKey="Sha, Zhenxia" sort="Sha, Zhenxia" uniqKey="Sha Z" first="Zhenxia" last="Sha">Zhenxia Sha</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and shazhenxia@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author></analytic><series><title level="j">Physiological genomics</title><idno type="eISSN">1531-2267</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated >60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27199458</PMID><DateCreated><Year>2016</Year><Month>07</Month><Day>02</Day></DateCreated><DateRevised><Year>2016</Year><Month>07</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1531-2267</ISSN><JournalIssue CitedMedium="Internet"><Volume>48</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>Physiological genomics</Title><ISOAbbreviation>Physiol. Genomics</ISOAbbreviation></Journal><ArticleTitle>Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads.</ArticleTitle><Pagination><MedlinePgn>464-76</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1152/physiolgenomics.00113.2015</ELocationID><Abstract><AbstractText>As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated >60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.</AbstractText><CopyrightInformation>Copyright © 2016 the American Physiological Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yadong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Yongtao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Hangzhou Qiandaohu Xunlong Sci-Tech Development Company Limited, Quzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shao</LastName><ForeName>Changwei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Lei</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Hangzhou Qiandaohu Xunlong Sci-Tech Development Company Limited, Quzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xuejie</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Mengjun</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sha</LastName><ForeName>Zhenxia</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Aoshanwei Town, Jimo, Qingdao, China; and shazhenxia@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Physiol Genomics</MedlineTA><NlmUniqueID>9815683</NlmUniqueID><ISSNLinking>1094-8341</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Russian sturgeon</Keyword><Keyword MajorTopicYN="N">gonad</Keyword><Keyword MajorTopicYN="N">sex-related genes</Keyword><Keyword MajorTopicYN="N">transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>11</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27199458</ArticleId><ArticleId IdType="pii">physiolgenomics.00113.2015</ArticleId><ArticleId IdType="doi">10.1152/physiolgenomics.00113.2015</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Oman</li><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Xuejie" sort="Chen, Xuejie" uniqKey="Chen X" first="Xuejie" last="Chen">Xuejie Chen</name><name sortKey="Chen, Yadong" sort="Chen, Yadong" uniqKey="Chen Y" first="Yadong" last="Chen">Yadong Chen</name><name sortKey="Yu, Mengjun" sort="Yu, Mengjun" uniqKey="Yu M" first="Mengjun" last="Yu">Mengjun Yu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Xia, Yongtao" sort="Xia, Yongtao" uniqKey="Xia Y" first="Yongtao" last="Xia">Yongtao Xia</name></noRegion><name sortKey="Han, Lei" sort="Han, Lei" uniqKey="Han L" first="Lei" last="Han">Lei Han</name><name sortKey="Shao, Changwei" sort="Shao, Changwei" uniqKey="Shao C" first="Changwei" last="Shao">Changwei Shao</name></country><country name="Oman"><noRegion><name sortKey="Sha, Zhenxia" sort="Sha, Zhenxia" uniqKey="Sha Z" first="Zhenxia" last="Sha">Zhenxia Sha</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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