Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus).
Identifieur interne : 000017 ( PubMed/Checkpoint ); précédent : 000016; suivant : 000018Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus).
Auteurs : Amin Golpour [République tchèque] ; Martin Pšeni Ka [République tchèque] ; Hamid Niksirat [République tchèque]Source :
- Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [ 1435-8115 ] ; 2016.
Abstract
Calcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate-pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.
DOI: 10.1017/S1431927616011958
PubMed: 27866505
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Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany,Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Research Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Niksirat, Hamid" sort="Niksirat, Hamid" uniqKey="Niksirat H" first="Hamid" last="Niksirat">Hamid Niksirat</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Research Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany,Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Research Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada</title><idno type="eISSN">1435-8115</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Calcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate-pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27866505</PMID><DateCreated><Year>2016</Year><Month>11</Month><Day>21</Day></DateCreated><DateRevised><Year>2016</Year><Month>12</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1435-8115</ISSN><JournalIssue CitedMedium="Internet"><Volume>22</Volume><Issue>6</Issue><PubDate><Year>2016</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada</Title><ISOAbbreviation>Microsc. Microanal.</ISOAbbreviation></Journal><ArticleTitle>Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus).</ArticleTitle><Pagination><MedlinePgn>1155-1161</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1017/S1431927616011958</ELocationID><Abstract><AbstractText NlmCategory="UNASSIGNED">Calcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate-pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Golpour</LastName><ForeName>Amin</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Research Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany,Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pšenička</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Research Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany,Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niksirat</LastName><ForeName>Hamid</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,Research Institute of Fish Culture and Hydrobiology,Zátiší 728/II,389 25 Vodňany,Czech Republic.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>11</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microsc Microanal</MedlineTA><NlmUniqueID>9712707</NlmUniqueID><ISSNLinking>1431-9276</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">calcium</Keyword><Keyword MajorTopicYN="N">oxalate–pyroantimonate</Keyword><Keyword MajorTopicYN="N">spermatozoon</Keyword><Keyword MajorTopicYN="N">subcellular localization</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27866505</ArticleId><ArticleId IdType="pii">S1431927616011958</ArticleId><ArticleId IdType="doi">10.1017/S1431927616011958</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République tchèque</li></country></list><tree><country name="République tchèque"><noRegion><name sortKey="Golpour, Amin" sort="Golpour, Amin" uniqKey="Golpour A" first="Amin" last="Golpour">Amin Golpour</name></noRegion><name sortKey="Niksirat, Hamid" sort="Niksirat, Hamid" uniqKey="Niksirat H" first="Hamid" last="Niksirat">Hamid Niksirat</name><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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