Expression Patterns of Atlantic Sturgeon (Acipenser oxyrinchus) During Embryonic Development.
Identifieur interne : 000008 ( PubMed/Checkpoint ); précédent : 000007; suivant : 000009Expression Patterns of Atlantic Sturgeon (Acipenser oxyrinchus) During Embryonic Development.
Auteurs : Elisavet Kaitetzidou [Grèce] ; Arne Ludwig [Allemagne] ; Jörn Gessner [Allemagne] ; Elena Sarropoulou [Grèce]Source :
- G3 (Bethesda, Md.) [ 2160-1836 ] ; 2017.
Abstract
During teleost ontogeny the larval and embryonic stages are key stages, since failure during this period of tissue differentiation may cause malformations, developmental delays, poor growth, and massive mortalities. Despite the rapid advances in sequencing technologies, the molecular backgrounds of the development of economically important but endangered fish species like the Atlantic sturgeon (Acipenser oxyrinchus) have not yet been thoroughly investigated. The current study examines the differential expression of transcripts involved in embryonic development of the Atlantic sturgeon. Addressing this goal, a reference transcriptome comprising eight stages was generated using an Illumina HiSequation 2500 platform. The constructed de novo assembly counted to 441,092 unfiltered and 179,564 filtered transcripts. Subsequently, the expression profile of four developmental stages ranging from early (gastrula) to late stages of prelarval development [2 d posthatching (dph)] were investigated applying an Illumina MiSeq platform. Differential expression analysis revealed distinct expression patterns among stages, especially between the two early and the two later stages. Transcripts upregulated at the two early stages were mainly enriched in transcripts linked to developmental processes, while transcripts expressed at the last two stages were mainly enriched in transcripts important to muscle contraction. Furthermore, important stage-specific expression has been detected for the hatching stage with transcripts enriched in molecule transport, and for the 2 dph stage with transcripts enriched in visual perception and lipid digestion. Our investigation represents a significant contribution to the understanding of Atlantic sturgeon embryonic development, and transcript characterization along with the differential expression results will significantly contribute to sturgeon research and aquaculture.
DOI: 10.1534/g3.116.036699
PubMed: 27974440
Affiliations:
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level="j">G3 (Bethesda, Md.)</title><idno type="eISSN">2160-1836</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">During teleost ontogeny the larval and embryonic stages are key stages, since failure during this period of tissue differentiation may cause malformations, developmental delays, poor growth, and massive mortalities. Despite the rapid advances in sequencing technologies, the molecular backgrounds of the development of economically important but endangered fish species like the Atlantic sturgeon (Acipenser oxyrinchus) have not yet been thoroughly investigated. The current study examines the differential expression of transcripts involved in embryonic development of the Atlantic sturgeon. Addressing this goal, a reference transcriptome comprising eight stages was generated using an Illumina HiSequation 2500 platform. The constructed de novo assembly counted to 441,092 unfiltered and 179,564 filtered transcripts. Subsequently, the expression profile of four developmental stages ranging from early (gastrula) to late stages of prelarval development [2 d posthatching (dph)] were investigated applying an Illumina MiSeq platform. Differential expression analysis revealed distinct expression patterns among stages, especially between the two early and the two later stages. Transcripts upregulated at the two early stages were mainly enriched in transcripts linked to developmental processes, while transcripts expressed at the last two stages were mainly enriched in transcripts important to muscle contraction. Furthermore, important stage-specific expression has been detected for the hatching stage with transcripts enriched in molecule transport, and for the 2 dph stage with transcripts enriched in visual perception and lipid digestion. Our investigation represents a significant contribution to the understanding of Atlantic sturgeon embryonic development, and transcript characterization along with the differential expression results will significantly contribute to sturgeon research and aquaculture.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27974440</PMID><DateCreated><Year>2016</Year><Month>12</Month><Day>15</Day></DateCreated><DateRevised><Year>2017</Year><Month>02</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2160-1836</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>2</Issue><PubDate><Year>2017</Year><Month>Feb</Month><Day>09</Day></PubDate></JournalIssue><Title>G3 (Bethesda, Md.)</Title><ISOAbbreviation>G3 (Bethesda)</ISOAbbreviation></Journal><ArticleTitle>Expression Patterns of Atlantic Sturgeon (Acipenser oxyrinchus) During Embryonic Development.</ArticleTitle><Pagination><MedlinePgn>533-542</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1534/g3.116.036699</ELocationID><Abstract><AbstractText NlmCategory="UNASSIGNED">During teleost ontogeny the larval and embryonic stages are key stages, since failure during this period of tissue differentiation may cause malformations, developmental delays, poor growth, and massive mortalities. Despite the rapid advances in sequencing technologies, the molecular backgrounds of the development of economically important but endangered fish species like the Atlantic sturgeon (Acipenser oxyrinchus) have not yet been thoroughly investigated. The current study examines the differential expression of transcripts involved in embryonic development of the Atlantic sturgeon. Addressing this goal, a reference transcriptome comprising eight stages was generated using an Illumina HiSequation 2500 platform. The constructed de novo assembly counted to 441,092 unfiltered and 179,564 filtered transcripts. Subsequently, the expression profile of four developmental stages ranging from early (gastrula) to late stages of prelarval development [2 d posthatching (dph)] were investigated applying an Illumina MiSeq platform. Differential expression analysis revealed distinct expression patterns among stages, especially between the two early and the two later stages. Transcripts upregulated at the two early stages were mainly enriched in transcripts linked to developmental processes, while transcripts expressed at the last two stages were mainly enriched in transcripts important to muscle contraction. Furthermore, important stage-specific expression has been detected for the hatching stage with transcripts enriched in molecule transport, and for the 2 dph stage with transcripts enriched in visual perception and lipid digestion. Our investigation represents a significant contribution to the understanding of Atlantic sturgeon embryonic development, and transcript characterization along with the differential expression results will significantly contribute to sturgeon research and aquaculture.</AbstractText><CopyrightInformation>Copyright © 2017 Kaitetzidou et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kaitetzidou</LastName><ForeName>Elisavet</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute for Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, Greece.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Biology, Faculty of Science, Aristotle University of Thessaloniki, 54124, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ludwig</LastName><ForeName>Arne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Evolutionary Genetics Research Group, Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gessner</LastName><ForeName>Jörn</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Biology and Ecology of Fishes, Leibniz Institute for Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sarropoulou</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute for Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, Greece sarris@hcmr.gr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>02</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>G3 (Bethesda)</MedlineTA><NlmUniqueID>101566598</NlmUniqueID><ISSNLinking>2160-1836</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Acipenser</Keyword><Keyword MajorTopicYN="N">RNA-seq</Keyword><Keyword MajorTopicYN="N">development</Keyword><Keyword MajorTopicYN="N">embryogenesis</Keyword><Keyword MajorTopicYN="N">hatching</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27974440</ArticleId><ArticleId IdType="pii">g3.116.036699</ArticleId><ArticleId IdType="doi">10.1534/g3.116.036699</ArticleId><ArticleId IdType="pmc">PMC5295599</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Grèce</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><country name="Grèce"><noRegion><name sortKey="Kaitetzidou, Elisavet" sort="Kaitetzidou, Elisavet" uniqKey="Kaitetzidou E" first="Elisavet" last="Kaitetzidou">Elisavet Kaitetzidou</name></noRegion><name sortKey="Sarropoulou, Elena" sort="Sarropoulou, Elena" uniqKey="Sarropoulou E" first="Elena" last="Sarropoulou">Elena Sarropoulou</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Ludwig, Arne" sort="Ludwig, Arne" uniqKey="Ludwig A" first="Arne" last="Ludwig">Arne Ludwig</name></region><name sortKey="Gessner, Jorn" sort="Gessner, Jorn" uniqKey="Gessner J" first="Jörn" last="Gessner">Jörn Gessner</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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