EsturgeonV1, PascalFrancis, Curation, bibRecord, 000233

Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

Identifieur interne : 000233 ( PascalFrancis/Curation ); précédent : 000232; suivant : 000234

Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

Auteurs : Andor Doszpoly [Hongrie] ; Endre R. Kovacs [Hongrie] ; Giuseppe Bovo [Italie] ; Scott E. Lapatra [États-Unis] ; Balazs Harrach [Hongrie] ; Maria Benko [Hongrie]

Source :

Archives of virology [ 0304-8608 ] ; 2008.

RBID : Pascal:09-0114910

Descripteurs français

Pascal (Inist)
- Herpesviridae, Réaction chaîne polymérase, Méthode, DNA-directed DNA polymerase, Gène, Syndrome de MELAS.

English descriptors

KwdEn :
- DNA-directed DNA polymerase, Gene, Herpesviridae, MELAS syndrome, Method, Polymerase chain reaction.

Abstract

A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictaluri-virus in the new family Alloherpesviridae.

A01	`01`	`1`		`@0 0304-8608`
A03		`1`		`@0 Arch. virol.`
A05				`@2 153`
A06				`@2 11`
A08	`01`	`1`	`ENG`	`@1 Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses`
A11	`01`	`1`		`@1 DOSZPOLY (Andor)`
A11	`02`	`1`		`@1 KOVACS (Endre R.)`
A11	`03`	`1`		`@1 BOVO (Giuseppe)`
A11	`04`	`1`		`@1 LAPATRA (Scott E.)`
A11	`05`	`1`		`@1 HARRACH (Balazs)`
A11	`06`	`1`		`@1 BENKO (Maria)`
A14	`01`			`@1 Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18 @2 1581 Budapest @3 HUN @Z 1 aut. @Z 2 aut. @Z 5 aut. @Z 6 aut.`
A14	`02`			`@1 Istituto Zooprofilattico Sperimentale delle Venezie @2 Legnaro @3 ITA @Z 3 aut.`
A14	`03`			`@1 Research Division, Clear Springs Foods Inc @2 Buhl, ID @3 USA @Z 4 aut.`
A20				`@1 2123-2127`
A21				`@1 2008`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 6355 @5 354000185391150180`
A44				`@0 0000 @1 © 2009 INIST-CNRS. All rights reserved.`
A45				`@0 37 ref.`
A47	`01`	`1`		`@0 09-0114910`
A60				`@1 P @3 CC`
A61				`@0 A`
A64	`01`	`1`		`@0 Archives of virology`
A66	`01`			`@0 AUT`
C01	`01`		`ENG`	@0 A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictaluri-virus in the new family Alloherpesviridae.
C02	`01`	`X`		`@0 002A05C10`
C02	`02`	`X`		`@0 002A05C08`
C03	`01`	`X`	`FRE`	`@0 Herpesviridae @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Herpesviridae @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Herpesviridae @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Réaction chaîne polymérase @5 05`
C03	`02`	`X`	`ENG`	`@0 Polymerase chain reaction @5 05`
C03	`02`	`X`	`SPA`	`@0 Reacción cadena polimerasa @5 05`
C03	`03`	`X`	`FRE`	`@0 Méthode @5 06`
C03	`03`	`X`	`ENG`	`@0 Method @5 06`
C03	`03`	`X`	`SPA`	`@0 Método @5 06`
C03	`04`	`X`	`FRE`	`@0 DNA-directed DNA polymerase @2 FE @5 07`
C03	`04`	`X`	`ENG`	`@0 DNA-directed DNA polymerase @2 FE @5 07`
C03	`04`	`X`	`SPA`	`@0 DNA-directed DNA polymerase @2 FE @5 07`
C03	`05`	`X`	`FRE`	`@0 Gène @5 08`
C03	`05`	`X`	`ENG`	`@0 Gene @5 08`
C03	`05`	`X`	`SPA`	`@0 Gen @5 08`
C03	`06`	`X`	`FRE`	`@0 Syndrome de MELAS @2 NM @5 14`
C03	`06`	`X`	`ENG`	`@0 MELAS syndrome @2 NM @5 14`
C03	`06`	`X`	`SPA`	`@0 MELAS síndrome @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`01`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`01`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nucleotidyltransferases @2 FE`
C07	`02`	`X`	`ENG`	`@0 Nucleotidyltransferases @2 FE`
C07	`02`	`X`	`SPA`	`@0 Nucleotidyltransferases @2 FE`
C07	`03`	`X`	`FRE`	`@0 Transferases @2 FE`
C07	`03`	`X`	`ENG`	`@0 Transferases @2 FE`
C07	`03`	`X`	`SPA`	`@0 Transferases @2 FE`
C07	`04`	`X`	`FRE`	`@0 Enzyme @2 FE`
C07	`04`	`X`	`ENG`	`@0 Enzyme @2 FE`
C07	`04`	`X`	`SPA`	`@0 Enzima @2 FE`
N21				`@1 082`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Pascal:09-0114910

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses</title>
<author><name sortKey="Doszpoly, Andor" sort="Doszpoly, Andor" uniqKey="Doszpoly A" first="Andor" last="Doszpoly">Andor Doszpoly</name>
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<front><div type="abstract" xml:lang="en">A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictaluri-virus in the new family Alloherpesviridae.</div>
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<fA14 i1="01"><s1>Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18</s1>
<s2>1581 Budapest</s2>
<s3>HUN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
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<fA14 i1="02"><s1>Istituto Zooprofilattico Sperimentale delle Venezie</s1>
<s2>Legnaro</s2>
<s3>ITA</s3>
<sZ>3 aut.</sZ>
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<fA14 i1="03"><s1>Research Division, Clear Springs Foods Inc</s1>
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<sZ>4 aut.</sZ>
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<fC01 i1="01" l="ENG"><s0>A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictaluri-virus in the new family Alloherpesviridae.</s0>
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<s5>01</s5>
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<s5>05</s5>
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<s5>06</s5>
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<s5>06</s5>
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<s5>06</s5>
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<s2>FE</s2>
<s5>07</s5>
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<fC03 i1="04" i2="X" l="ENG"><s0>DNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>07</s5>
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<fC03 i1="04" i2="X" l="SPA"><s0>DNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>07</s5>
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<s5>08</s5>
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<s2>FE</s2>
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<s2>FE</s2>
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<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fN21><s1>082</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
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   |flux=    PascalFrancis
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   |type=    RBID
   |clé=     Pascal:09-0114910
   |texte=   Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses
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Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

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