The effect of temperature stress on development and heat-shock protein expression in larval green sturgeon (A cipenser medirostris)
Identifieur interne : 000190 ( PascalFrancis/Curation ); précédent : 000189; suivant : 000191The effect of temperature stress on development and heat-shock protein expression in larval green sturgeon (A cipenser medirostris)
Auteurs : Inge Werner [États-Unis] ; Javier Linares-Casenave [États-Unis] ; Joel P. Van Eenennaam [États-Unis] ; Serge I. Doroshov [États-Unis]Source :
- Environmental biology of fishes [ 0378-1909 ] ; 2007.
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- Pascal (Inist)
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Abstract
Water temperature is an important environmental variable influencing the distribution and health of coldwater fishes such as the green sturgeon, Acipenser medirostris. In this study, we investigated if larval sturgeon were able to tolerate or recover from acute, non-lethal temperature stress that commonly causes deformed notochords, and sought to identify the role of heat-shock proteins (hsp) in stress tolerance. The hsp response is one of the most important cellular mechanisms to prevent the damaging effects of thermal cellular stress, and differences in the ability to over-express hsps during stressful conditions may be associated with an organism's vulnerability and the extent of thermal injury. In this study, newly hatched larvae were maintained at 17°C (control), or exposed to (a) 26°C for 3 d then maintained at 17°C until yolk-sac absorption or (b) 26°C until yolk-sac absorption. Individuals with deformed notochords were counted, and hsp60, 72, 78 and 89 were analyzed in both normal and deformed larvae by western blotting. Approximately 33% of fish developed curved notochords within the first 3 d of exposure to 26°C. After transfer to cool water 16.5% showed deformities at stage 45, suggesting a significant number of larvae had recovered. Hsp levels remained elevated for at least 9 days after termination of heat-exposure. Overall, percentage of deformed larvae, and hsp72/hsp78 levels were highest in fish continuously exposed to 26°C until yolk-sac absorption. Deformed individuals had significantly higher expression levels of hsp72 and hsp78, and lower hsp60 levels than normal larvae. We conclude that expression of hsp72 and hsp78 and potentially hsp60 are linked to phenotypic variation in the response and vulnerability of green sturgeon larvae to thermal stress.
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Van Eenennaam</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Animal Science, University of California, One Shields Avenue</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I." last="Doroshov">Serge I. Doroshov</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Animal Science, University of California, One Shields Avenue</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0412089</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0412089 INIST</idno><idno type="RBID">Pascal:07-0412089</idno><idno type="wicri:Area/PascalFrancis/Corpus">000186</idno><idno type="wicri:Area/PascalFrancis/Curation">000190</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The effect of temperature stress on development and heat-shock protein expression in larval green sturgeon (A cipenser medirostris)</title><author><name sortKey="Werner, Inge" sort="Werner, Inge" uniqKey="Werner I" first="Inge" last="Werner">Inge Werner</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anatomy, Physiology and Cell Biology: School of Veterinary Medicine, University of California, One Shields Avenue</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>1 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Linares Casenave, Javier" sort="Linares Casenave, Javier" uniqKey="Linares Casenave J" first="Javier" last="Linares-Casenave">Javier Linares-Casenave</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Animal Science, University of California, One Shields Avenue</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Van Eenennaam, Joel P" sort="Van Eenennaam, Joel P" uniqKey="Van Eenennaam J" first="Joel P." last="Van Eenennaam">Joel P. Van Eenennaam</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Animal Science, University of California, One Shields Avenue</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I." last="Doroshov">Serge I. Doroshov</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Animal Science, University of California, One Shields Avenue</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author></analytic><series><title level="j" type="main">Environmental biology of fishes</title><title level="j" type="abbreviated">Environ. biol. fishes</title><idno type="ISSN">0378-1909</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Environmental biology of fishes</title><title level="j" type="abbreviated">Environ. biol. fishes</title><idno type="ISSN">0378-1909</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Development</term><term>Heat shock protein</term><term>Larva</term><term>Pisces</term><term>Stress</term><term>Temperature effect</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Effet température</term><term>Stress</term><term>Protéine choc thermique</term><term>Larve</term><term>Développement</term><term>Pisces</term><term>Acipenser</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Water temperature is an important environmental variable influencing the distribution and health of coldwater fishes such as the green sturgeon, Acipenser medirostris. In this study, we investigated if larval sturgeon were able to tolerate or recover from acute, non-lethal temperature stress that commonly causes deformed notochords, and sought to identify the role of heat-shock proteins (hsp) in stress tolerance. The hsp response is one of the most important cellular mechanisms to prevent the damaging effects of thermal cellular stress, and differences in the ability to over-express hsps during stressful conditions may be associated with an organism's vulnerability and the extent of thermal injury. In this study, newly hatched larvae were maintained at 17°C (control), or exposed to (a) 26°C for 3 d then maintained at 17°C until yolk-sac absorption or (b) 26°C until yolk-sac absorption. Individuals with deformed notochords were counted, and hsp60, 72, 78 and 89 were analyzed in both normal and deformed larvae by western blotting. Approximately 33% of fish developed curved notochords within the first 3 d of exposure to 26°C. After transfer to cool water 16.5% showed deformities at stage 45, suggesting a significant number of larvae had recovered. Hsp levels remained elevated for at least 9 days after termination of heat-exposure. Overall, percentage of deformed larvae, and hsp72/hsp78 levels were highest in fish continuously exposed to 26°C until yolk-sac absorption. Deformed individuals had significantly higher expression levels of hsp72 and hsp78, and lower hsp60 levels than normal larvae. We conclude that expression of hsp72 and hsp78 and potentially hsp60 are linked to phenotypic variation in the response and vulnerability of green sturgeon larvae to thermal stress.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0378-1909</s0></fA01><fA02 i1="01"><s0>EBFID3</s0></fA02><fA03 i2="1"><s0>Environ. biol. fishes</s0></fA03><fA05><s2>79</s2></fA05><fA06><s2>3-4</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>The effect of temperature stress on development and heat-shock protein expression in larval green sturgeon (A cipenser medirostris)</s1></fA08><fA09 i1="01" i2="1" l="ENG"><s1>Green sturgeon</s1></fA09><fA11 i1="01" i2="1"><s1>WERNER (Inge)</s1></fA11><fA11 i1="02" i2="1"><s1>LINARES-CASENAVE (Javier)</s1></fA11><fA11 i1="03" i2="1"><s1>VAN EENENNAAM (Joel P.)</s1></fA11><fA11 i1="04" i2="1"><s1>DOROSHOV (Serge I.)</s1></fA11><fA12 i1="01" i2="1"><s1>KLIMLEY (A. 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