The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species
Identifieur interne :
000135 ( PascalFrancis/Curation );
précédent :
000134;
suivant :
000136
The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species
Auteurs : Akos Horvath [
États-Unis,
Hongrie] ;
William R. Wayman [
États-Unis] ;
Béla Urbanyi [
Hongrie] ;
Kent M. Ware [
États-Unis] ;
Jan C. Dean [
États-Unis] ;
Terrence R. Tiersch [
États-Unis]
Source :
-
Aquaculture : (Amsterdam) [ 0044-8486 ] ; 2005.
RBID : Pascal:05-0304219
Descripteurs français
English descriptors
Abstract
Successful sperm cryopreservation techniques have been developed for Eurasian sturgeon species; however, there is little information available on these techniques for North-American species. In this study, two sets of sperm cryopreservation experiments were carried out on the endangered shortnose sturgeon (Acipenser brevirostrum). In the first set, the cryoprotectants methanol (MeOH) and dimethyl sulfoxide (DMSO) were investigated using three concentrations (5%, 10% and 15%). The highest post-thaw motility was found using 5% DMSO (26±13%) while the use of 5% MeOH resulted in the highest rates for fertilization at the 4-cell stage (40±15%), neurulation (38±13%) and hatching (32±12%). In the second set, the Original Tsvetkova's extender (OT), Modified Tsvetkova's extender (MT) and modified Hanks' balanced salt solution (mHBSS) were investigated in combination with three MeOH concentrations. The highest post-thaw motility (18±10%), fertilization (18±11%) and hatching rates (17±12%) were observed with MT extender used in combination with 5% MeOH. In another set of experiments, the effects of two extenders (MT and mHBSS) and two concentrations of MeOH were investigated for sperm cryopreservation of pallid sturgeon (Scaphyrinchus albus). The highest post-thaw motility (70±10%) was observed using MT and 10% MeOH while MT and 5% MeOH yielded the highest rates of fertilization (88±6%) and hatching (73 ±14%). In general we conclude that although hyperosmotic conditions of extenders and cryoprotectants result in higher post-thaw motility, they seem to reduce the fertilizing ability of the sperm.
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| C01 | 01 | | ENG | @0 Successful sperm cryopreservation techniques have been developed for Eurasian sturgeon species; however, there is little information available on these techniques for North-American species. In this study, two sets of sperm cryopreservation experiments were carried out on the endangered shortnose sturgeon (Acipenser brevirostrum). In the first set, the cryoprotectants methanol (MeOH) and dimethyl sulfoxide (DMSO) were investigated using three concentrations (5%, 10% and 15%). The highest post-thaw motility was found using 5% DMSO (26±13%) while the use of 5% MeOH resulted in the highest rates for fertilization at the 4-cell stage (40±15%), neurulation (38±13%) and hatching (32±12%). In the second set, the Original Tsvetkova's extender (OT), Modified Tsvetkova's extender (MT) and modified Hanks' balanced salt solution (mHBSS) were investigated in combination with three MeOH concentrations. The highest post-thaw motility (18±10%), fertilization (18±11%) and hatching rates (17±12%) were observed with MT extender used in combination with 5% MeOH. In another set of experiments, the effects of two extenders (MT and mHBSS) and two concentrations of MeOH were investigated for sperm cryopreservation of pallid sturgeon (Scaphyrinchus albus). The highest post-thaw motility (70±10%) was observed using MT and 10% MeOH while MT and 5% MeOH yielded the highest rates of fertilization (88±6%) and hatching (73 ±14%). In general we conclude that although hyperosmotic conditions of extenders and cryoprotectants result in higher post-thaw motility, they seem to reduce the fertilizing ability of the sperm. |
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Le document en format XML
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</publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species</title>
<author><name sortKey="Horvath, Akos" sort="Horvath, Akos" uniqKey="Horvath A" first="Akos" last="Horvath">Akos Horvath</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, 2410 Ben Hur Road</s1>
<s2>LA 70820</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14><country>États-Unis</country>
</affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Fish Culture, Szent Istvan University, Páler K. u. 1</s1>
<s2>Gödöllö, 2103</s2>
<s3>HUN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14><country>Hongrie</country>
</affiliation><author><name sortKey="Wayman, William R" sort="Wayman, William R" uniqKey="Wayman W" first="William R." last="Wayman">William R. Wayman</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Warm Springs Fish Technology Center, Fish and Wildlife Service</s1>
<s2>5308 Spring St. Warm Springs, GA 31830</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
</inist:fA14><country>États-Unis</country>
</affiliation><author><name sortKey="Urbanyi, Bela" sort="Urbanyi, Bela" uniqKey="Urbanyi B" first="Béla" last="Urbanyi">Béla Urbanyi</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Fish Culture, Szent Istvan University, Páler K. u. 1</s1>
<s2>Gödöllö, 2103</s2>
<s3>HUN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14><country>Hongrie</country>
</affiliation><author><name sortKey="Ware, Kent M" sort="Ware, Kent M" uniqKey="Ware K" first="Kent M." last="Ware">Kent M. Ware</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Bears Bluff National Fish Hatchery, Fish and Wildlife Service, 7030 Bears Bluff Road</s1>
<s2>Wadmalaw Island, SC 29487</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
</inist:fA14><country>États-Unis</country>
</affiliation><author><name sortKey="Dean, Jan C" sort="Dean, Jan C" uniqKey="Dean J" first="Jan C." last="Dean">Jan C. Dean</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Natchitoches National Fish Hatchery, Fish and Wildlife Service, 615 South Drive</s1>
<s2>Natchitoches, LA 71457</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
</inist:fA14><country>États-Unis</country>
</affiliation><author><name sortKey="Tiersch, Terrence R" sort="Tiersch, Terrence R" uniqKey="Tiersch T" first="Terrence R." last="Tiersch">Terrence R. Tiersch</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, 2410 Ben Hur Road</s1>
<s2>LA 70820</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14><country>États-Unis</country>
</affiliation><series><title level="j" type="main">Aquaculture : (Amsterdam)</title>
<title level="j" type="abbreviated">Aquaculture : (Amst.)</title>
<idno type="ISSN">0044-8486</idno>
<imprint><date when="2005">2005</date>
</imprint><seriesStmt><title level="j" type="main">Aquaculture : (Amsterdam)</title>
<title level="j" type="abbreviated">Aquaculture : (Amst.)</title>
<idno type="ISSN">0044-8486</idno>
</seriesStmt><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser sturio</term>
<term>Aquaculture</term>
<term>Cryopreservation</term>
<term>North America</term>
<term>Spermatozoa</term>
</keywords><keywords scheme="Pascal" xml:lang="fr"><term>Spermatozoïde</term>
<term>Cryoconservation</term>
<term>Amérique du Nord</term>
<term>Aquiculture</term>
<term>Acipenser sturio</term>
</keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Aquiculture</term>
</keywords><front><div type="abstract" xml:lang="en">Successful sperm cryopreservation techniques have been developed for Eurasian sturgeon species; however, there is little information available on these techniques for North-American species. In this study, two sets of sperm cryopreservation experiments were carried out on the endangered shortnose sturgeon (Acipenser brevirostrum). In the first set, the cryoprotectants methanol (MeOH) and dimethyl sulfoxide (DMSO) were investigated using three concentrations (5%, 10% and 15%). The highest post-thaw motility was found using 5% DMSO (26±13%) while the use of 5% MeOH resulted in the highest rates for fertilization at the 4-cell stage (40±15%), neurulation (38±13%) and hatching (32±12%). In the second set, the Original Tsvetkova's extender (OT), Modified Tsvetkova's extender (MT) and modified Hanks' balanced salt solution (mHBSS) were investigated in combination with three MeOH concentrations. The highest post-thaw motility (18±10%), fertilization (18±11%) and hatching rates (17±12%) were observed with MT extender used in combination with 5% MeOH. In another set of experiments, the effects of two extenders (MT and mHBSS) and two concentrations of MeOH were investigated for sperm cryopreservation of pallid sturgeon (Scaphyrinchus albus). The highest post-thaw motility (70±10%) was observed using MT and 10% MeOH while MT and 5% MeOH yielded the highest rates of fertilization (88±6%) and hatching (73 ±14%). In general we conclude that although hyperosmotic conditions of extenders and cryoprotectants result in higher post-thaw motility, they seem to reduce the fertilizing ability of the sperm.</div>
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</fA06><fA08 i1="01" i2="1" l="ENG"><s1>The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species</s1>
</fA08><fA09 i1="01" i2="1" l="ENG"><s1>Genetics in Aquaculture VIII: Proceedings of the Eighth International Symposium on Genetics in Aquaculture hosted by the University of Chile and held at the city of Puerto Varas, Chile during the week of 9-15 November 2003</s1>
</fA09><fA11 i1="01" i2="1"><s1>HORVATH (Akos)</s1>
</fA11><fA11 i1="02" i2="1"><s1>WAYMAN (William R.)</s1>
</fA11><fA11 i1="03" i2="1"><s1>URBANYI (Béla)</s1>
</fA11><fA11 i1="04" i2="1"><s1>WARE (Kent M.)</s1>
</fA11><fA11 i1="05" i2="1"><s1>DEAN (Jan C.)</s1>
</fA11><fA11 i1="06" i2="1"><s1>TIERSCH (Terrence R.)</s1>
</fA11><fA12 i1="01" i2="1"><s1>NEIRA (Roberto)</s1>
<s9>ed.</s9>
</fA12><fA12 i1="02" i2="1"><s1>DIAZ (Nelson F.)</s1>
<s9>ed.</s9>
</fA12><fA14 i1="01"><s1>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, 2410 Ben Hur Road</s1>
<s2>LA 70820</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14><fA14 i1="02"><s1>Department of Fish Culture, Szent Istvan University, Páler K. u. 1</s1>
<s2>Gödöllö, 2103</s2>
<s3>HUN</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14><fA14 i1="03"><s1>Warm Springs Fish Technology Center, Fish and Wildlife Service</s1>
<s2>5308 Spring St. Warm Springs, GA 31830</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
</fA14><fA14 i1="04"><s1>Bears Bluff National Fish Hatchery, Fish and Wildlife Service, 7030 Bears Bluff Road</s1>
<s2>Wadmalaw Island, SC 29487</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
</fA14><fA14 i1="05"><s1>Natchitoches National Fish Hatchery, Fish and Wildlife Service, 615 South Drive</s1>
<s2>Natchitoches, LA 71457</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
</fA14><fA15 i1="01"><s1>Departamento de Producción Animal, Facultad de Ciencias Agronómicas, Universidad de Chile, Casilla 1004</s1>
<s2>Santiago</s2>
<s3>CHL</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</fA15><fA18 i1="01" i2="1"><s1>Foundation for Agrarian Innovation (FIA)</s1>
<s3>CHL</s3>
<s9>org-cong.</s9>
</fA18><fA18 i1="02" i2="1"><s1>Institute of Fisheries Development (IFOP)</s1>
<s3>CHL</s3>
<s9>org-cong.</s9>
</fA18><fA20><s1>243-251</s1>
</fA20><fA21><s1>2005</s1>
</fA21><fA23 i1="01"><s0>ENG</s0>
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<s2>15964</s2>
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</fA60><fA64 i1="01" i2="1"><s0>Aquaculture : (Amsterdam)</s0>
</fA64><fA66 i1="01"><s0>NLD</s0>
</fA66><fC01 i1="01" l="ENG"><s0>Successful sperm cryopreservation techniques have been developed for Eurasian sturgeon species; however, there is little information available on these techniques for North-American species. In this study, two sets of sperm cryopreservation experiments were carried out on the endangered shortnose sturgeon (Acipenser brevirostrum). In the first set, the cryoprotectants methanol (MeOH) and dimethyl sulfoxide (DMSO) were investigated using three concentrations (5%, 10% and 15%). The highest post-thaw motility was found using 5% DMSO (26±13%) while the use of 5% MeOH resulted in the highest rates for fertilization at the 4-cell stage (40±15%), neurulation (38±13%) and hatching (32±12%). In the second set, the Original Tsvetkova's extender (OT), Modified Tsvetkova's extender (MT) and modified Hanks' balanced salt solution (mHBSS) were investigated in combination with three MeOH concentrations. The highest post-thaw motility (18±10%), fertilization (18±11%) and hatching rates (17±12%) were observed with MT extender used in combination with 5% MeOH. In another set of experiments, the effects of two extenders (MT and mHBSS) and two concentrations of MeOH were investigated for sperm cryopreservation of pallid sturgeon (Scaphyrinchus albus). The highest post-thaw motility (70±10%) was observed using MT and 10% MeOH while MT and 5% MeOH yielded the highest rates of fertilization (88±6%) and hatching (73 ±14%). In general we conclude that although hyperosmotic conditions of extenders and cryoprotectants result in higher post-thaw motility, they seem to reduce the fertilizing ability of the sperm.</s0>
</fC01><fC02 i1="01" i2="X"><s0>002A36B01</s0>
</fC02><fC02 i1="02" i2="X"><s0>002A15B</s0>
</fC02><fC03 i1="01" i2="X" l="FRE"><s0>Spermatozoïde</s0>
<s5>01</s5>
</fC03><fC03 i1="01" i2="X" l="ENG"><s0>Spermatozoa</s0>
<s5>01</s5>
</fC03><fC03 i1="01" i2="X" l="SPA"><s0>Espermatozoide</s0>
<s5>01</s5>
</fC03><fC03 i1="02" i2="X" l="FRE"><s0>Cryoconservation</s0>
<s5>02</s5>
</fC03><fC03 i1="02" i2="X" l="ENG"><s0>Cryopreservation</s0>
<s5>02</s5>
</fC03><fC03 i1="02" i2="X" l="SPA"><s0>Crioconservación</s0>
<s5>02</s5>
</fC03><fC03 i1="03" i2="X" l="FRE"><s0>Amérique du Nord</s0>
<s2>NG</s2>
<s5>03</s5>
</fC03><fC03 i1="03" i2="X" l="ENG"><s0>North America</s0>
<s2>NG</s2>
<s5>03</s5>
</fC03><fC03 i1="03" i2="X" l="SPA"><s0>America del norte</s0>
<s2>NG</s2>
<s5>03</s5>
</fC03><fC03 i1="04" i2="X" l="FRE"><s0>Aquiculture</s0>
<s5>04</s5>
</fC03><fC03 i1="04" i2="X" l="ENG"><s0>Aquaculture</s0>
<s5>04</s5>
</fC03><fC03 i1="04" i2="X" l="SPA"><s0>Acuacultura</s0>
<s5>04</s5>
</fC03><fC03 i1="05" i2="X" l="FRE"><s0>Acipenser sturio</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03><fC03 i1="05" i2="X" l="ENG"><s0>Acipenser sturio</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03><fC03 i1="05" i2="X" l="SPA"><s0>Acipenser sturio</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03><fC07 i1="01" i2="X" l="FRE"><s0>Amérique</s0>
<s2>NG</s2>
</fC07><fC07 i1="01" i2="X" l="ENG"><s0>America</s0>
<s2>NG</s2>
</fC07><fC07 i1="01" i2="X" l="SPA"><s0>America</s0>
<s2>NG</s2>
</fC07><fC07 i1="02" i2="X" l="FRE"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07><fC07 i1="02" i2="X" l="ENG"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07><fC07 i1="02" i2="X" l="SPA"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07><fC07 i1="03" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07><fC07 i1="03" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07><fC07 i1="03" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07><fC07 i1="04" i2="X" l="FRE"><s0>Acipenseridae</s0>
<s4>INC</s4>
<s5>70</s5>
</fC07><fN21><s1>213</s1>
</fN21><fN44 i1="01"><s1>OTO</s1>
</fN44><fN82><s1>OTO</s1>
</fN82><pR><fA30 i1="01" i2="1" l="ENG"><s1>ISGA International Symposium on Genetics in Aquaculture</s1>
<s2>8</s2>
<s3>Puerto Varas CHL</s3>
<s4>2003-11-09</s4>
</fA30>
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