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Effect of ovarian stage on plasma vitellogenin and calcium in cultured white sturgeon

Identifieur interne : 000083 ( PascalFrancis/Curation ); précédent : 000082; suivant : 000084

Effect of ovarian stage on plasma vitellogenin and calcium in cultured white sturgeon

Auteurs : J. Linares-Casenave [États-Unis] ; K. J. Kroll [États-Unis] ; J. P. Van Eenennaam [États-Unis] ; S. I. Doroshov [États-Unis]

Source :

RBID : Pascal:03-0326515

Descripteurs français

English descriptors

Abstract

We developed an enzyme immunoassay for plasma vitellogenin (VTG) of white sturgeon Acipenser transmontanus, cultured in California. The assay, based on polyclonal rabbit antibody to purified VTG, was tested for specificity and applied on sturgeon broodstocks (age 4-12 years) reared at three locations. Plasma VTG concentrations were correlated with total plasma calcium and stage of ovarian maturity. Females in the previtellogenic ovarian stage and at the onset of yolk deposition had low VTG concentrations, but plasma VTG and calcium increased after onset of yolk deposition in the oocytes. Total plasma calcium exhibited a linear relationship with plasma VTG. Concentrations of both metabolites in plasma discriminate previtellogenic and vitellogenic females, but fail to discriminate specific stage of ovarian vitellogenesis. The immunoassay for plasma VTG or flame photometry for total plasma calcium can be utilized to segregate vitellogenic females in cultured sturgeon broodstocks.
pA  
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A02 01      @0 AQCLAL
A03   1    @0 Aquaculture : (Amst.)
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A08 01  1  ENG  @1 Effect of ovarian stage on plasma vitellogenin and calcium in cultured white sturgeon
A11 01  1    @1 LINARES-CASENAVE (J.)
A11 02  1    @1 KROLL (K. J.)
A11 03  1    @1 VAN EENENNAAM (J. P.)
A11 04  1    @1 DOROSHOV (S. I.)
A14 01      @1 Department of Animal Science, University of Califonia, One Shield Avenue @2 Davis, CA 95616-8521 @3 USA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.
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A21       @1 2003
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A43 01      @1 INIST @2 15964 @5 354000110880450490
A44       @0 0000 @1 © 2003 INIST-CNRS. All rights reserved.
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A47 01  1    @0 03-0326515
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A64 01  1    @0 Aquaculture : (Amsterdam)
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C01 01    ENG  @0 We developed an enzyme immunoassay for plasma vitellogenin (VTG) of white sturgeon Acipenser transmontanus, cultured in California. The assay, based on polyclonal rabbit antibody to purified VTG, was tested for specificity and applied on sturgeon broodstocks (age 4-12 years) reared at three locations. Plasma VTG concentrations were correlated with total plasma calcium and stage of ovarian maturity. Females in the previtellogenic ovarian stage and at the onset of yolk deposition had low VTG concentrations, but plasma VTG and calcium increased after onset of yolk deposition in the oocytes. Total plasma calcium exhibited a linear relationship with plasma VTG. Concentrations of both metabolites in plasma discriminate previtellogenic and vitellogenic females, but fail to discriminate specific stage of ovarian vitellogenesis. The immunoassay for plasma VTG or flame photometry for total plasma calcium can be utilized to segregate vitellogenic females in cultured sturgeon broodstocks.
C02 01  X    @0 002A36B03A
C03 01  X  FRE  @0 Plasma @5 01
C03 01  X  ENG  @0 Plasma @5 01
C03 01  X  SPA  @0 Plasma @5 01
C03 02  X  FRE  @0 Vitellogénine @5 02
C03 02  X  ENG  @0 Vitellogenin @5 02
C03 02  X  SPA  @0 Vitelogenina @5 02
C03 03  X  FRE  @0 Calcium @2 NC @2 FR @5 03
C03 03  X  ENG  @0 Calcium @2 NC @2 FR @5 03
C03 03  X  SPA  @0 Calcio @2 NC @2 FR @5 03
C03 04  X  FRE  @0 Maturité sexuelle @5 04
C03 04  X  ENG  @0 Sexual maturity @5 04
C03 04  X  SPA  @0 Madurez sexual @5 04
C03 05  X  FRE  @0 Ovaire @5 05
C03 05  X  ENG  @0 Ovary @5 05
C03 05  X  SPA  @0 Ovario @5 05
C03 06  X  FRE  @0 Technique ELISA @5 06
C03 06  X  ENG  @0 ELISA assay @5 06
C03 06  X  SPA  @0 Técnica ELISA @5 06
C03 07  X  FRE  @0 Stock @5 07
C03 07  X  ENG  @0 Stock @5 07
C03 07  X  SPA  @0 Existencias @5 07
C03 08  X  FRE  @0 Reproduction @5 08
C03 08  X  ENG  @0 Reproduction @5 08
C03 08  X  SPA  @0 Reproducción @5 08
C03 09  X  FRE  @0 Pisciculture @5 09
C03 09  X  ENG  @0 Pisciculture @5 09
C03 09  X  SPA  @0 Piscicultura @5 09
C03 10  X  FRE  @0 Acipenser transmontanus @2 NS @5 55
C03 10  X  ENG  @0 Acipenser transmontanus @2 NS @5 55
C03 10  X  SPA  @0 Acipenser transmontanus @2 NS @5 55
C07 01  X  FRE  @0 Appareil génital femelle @5 21
C07 01  X  ENG  @0 Female genital system @5 21
C07 01  X  SPA  @0 Aparato genital hembra @5 21
C07 02  X  FRE  @0 Pisces @2 NS
C07 02  X  ENG  @0 Pisces @2 NS
C07 02  X  SPA  @0 Pisces @2 NS
C07 03  X  FRE  @0 Vertebrata @2 NS
C07 03  X  ENG  @0 Vertebrata @2 NS
C07 03  X  SPA  @0 Vertebrata @2 NS
C07 04  X  FRE  @0 Acipenseridae @2 NS @4 INC @5 70
N21       @1 223
N82       @1 PSI

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Pascal:03-0326515

Le document en format XML

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<div type="abstract" xml:lang="en">We developed an enzyme immunoassay for plasma vitellogenin (VTG) of white sturgeon Acipenser transmontanus, cultured in California. The assay, based on polyclonal rabbit antibody to purified VTG, was tested for specificity and applied on sturgeon broodstocks (age 4-12 years) reared at three locations. Plasma VTG concentrations were correlated with total plasma calcium and stage of ovarian maturity. Females in the previtellogenic ovarian stage and at the onset of yolk deposition had low VTG concentrations, but plasma VTG and calcium increased after onset of yolk deposition in the oocytes. Total plasma calcium exhibited a linear relationship with plasma VTG. Concentrations of both metabolites in plasma discriminate previtellogenic and vitellogenic females, but fail to discriminate specific stage of ovarian vitellogenesis. The immunoassay for plasma VTG or flame photometry for total plasma calcium can be utilized to segregate vitellogenic females in cultured sturgeon broodstocks.</div>
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<fC07 i1="03" i2="X" l="ENG">
<s0>Vertebrata</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="SPA">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Acipenseridae</s0>
<s2>NS</s2>
<s4>INC</s4>
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<fN21>
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</fN21>
<fN82>
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