EsturgeonV1, PascalFrancis, Corpus, bibRecord, 000080

Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon

Identifieur interne : 000080 ( PascalFrancis/Corpus ); précédent : 000079; suivant : 000081

Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon

Auteurs : T. Kurobe ; K. T. Kwak ; E. Macconnell ; T. S. Mcdowell ; F. O. Mardones ; R. P. Hedrick

Source :

Diseases of aquatic organisms [ 0177-5103 ] ; 2010.

RBID : Pascal:11-0426955

Descripteurs français

Pascal (Inist)
- Développement, Réaction chaîne polymérase, Iridovirus, Infection, Captivité, Cours eau, Missouri, Pisces, Acipenser, Scaphirhynchus albus.

English descriptors

KwdEn :
- Captivity, Development, Infection, Iridovirus, Missouri, Pisces, Polymerase chain reaction, Stream.

Abstract

The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A02	`01`			`@0 DAOREO`
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A05				`@2 93`
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A08	`01`	`1`	`ENG`	`@1 Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon`
A11	`01`	`1`		`@1 KUROBE (T.)`
A11	`02`	`1`		`@1 KWAK (K. T.)`
A11	`03`	`1`		`@1 MACCONNELL (E.)`
A11	`04`	`1`		`@1 MCDOWELL (T. S.)`
A11	`05`	`1`		`@1 MARDONES (F. O.)`
A11	`06`	`1`		`@1 HEDRICK (R. P.)`
A14	`01`			`@1 Department of Medicine and Epidemiology, University of California @2 Davis, California 95616 @3 USA @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 6 aut.`
A14	`02`			`@1 Center for Animal Disease Modeling and Surveillance (CADMS), School of Veterinary Medicine, University of California @2 Davis, California 95616 @3 USA @Z 5 aut.`
A14	`03`			`@1 US Fish and Wildlife Service, Fish Health Center @2 Bozeman, Montana 59715 @3 USA @Z 3 aut.`
A20				`@1 31-42`
A21				`@1 2010`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 21304 @5 354000194047800030`
A44				`@0 0000 @1 © 2011 INIST-CNRS. All rights reserved.`
A45				`@0 1 p.1/4`
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A61				`@0 A`
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C01	`01`		`ENG`	@0 The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.
C02	`01`	`X`		`@0 002A14B04C`
C02	`02`	`X`		`@0 002A15B`
C03	`01`	`X`	`FRE`	`@0 Développement @5 01`
C03	`01`	`X`	`ENG`	`@0 Development @5 01`
C03	`01`	`X`	`SPA`	`@0 Desarrollo @5 01`
C03	`02`	`X`	`FRE`	`@0 Réaction chaîne polymérase @5 02`
C03	`02`	`X`	`ENG`	`@0 Polymerase chain reaction @5 02`
C03	`02`	`X`	`SPA`	`@0 Reacción cadena polimerasa @5 02`
C03	`03`	`X`	`FRE`	`@0 Iridovirus @2 NW @5 03`
C03	`03`	`X`	`ENG`	`@0 Iridovirus @2 NW @5 03`
C03	`03`	`X`	`SPA`	`@0 Iridovirus @2 NW @5 03`
C03	`04`	`X`	`FRE`	`@0 Infection @5 04`
C03	`04`	`X`	`ENG`	`@0 Infection @5 04`
C03	`04`	`X`	`SPA`	`@0 Infección @5 04`
C03	`05`	`X`	`FRE`	`@0 Captivité @5 05`
C03	`05`	`X`	`ENG`	`@0 Captivity @5 05`
C03	`05`	`X`	`SPA`	`@0 Cautividad @5 05`
C03	`06`	`X`	`FRE`	`@0 Cours eau @5 06`
C03	`06`	`X`	`ENG`	`@0 Stream @5 06`
C03	`06`	`X`	`SPA`	`@0 Curso agua @5 06`
C03	`07`	`X`	`FRE`	`@0 Missouri @2 NG @5 19`
C03	`07`	`X`	`ENG`	`@0 Missouri @2 NG @5 19`
C03	`07`	`X`	`SPA`	`@0 Misuri @2 NG @5 19`
C03	`08`	`X`	`FRE`	`@0 Pisces @2 NS @5 55`
C03	`08`	`X`	`ENG`	`@0 Pisces @2 NS @5 55`
C03	`08`	`X`	`SPA`	`@0 Pisces @2 NS @5 55`
C03	`09`	`X`	`FRE`	`@0 Acipenser @4 INC @5 64`
C03	`10`	`X`	`FRE`	`@0 Scaphirhynchus albus @4 INC @5 87`
C07	`01`	`X`	`FRE`	`@0 Iridoviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Iridoviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Iridoviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`02`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`02`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`03`	`X`	`FRE`	`@0 Etats-Unis @2 NG`
C07	`03`	`X`	`ENG`	`@0 United States @2 NG`
C07	`03`	`X`	`SPA`	`@0 Estados Unidos @2 NG`
C07	`04`	`X`	`FRE`	`@0 Amérique du Nord @2 NG`
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C07	`04`	`X`	`SPA`	`@0 America del norte @2 NG`
C07	`05`	`X`	`FRE`	`@0 Amérique @2 NG`
C07	`05`	`X`	`ENG`	`@0 America @2 NG`
C07	`05`	`X`	`SPA`	`@0 America @2 NG`
C07	`06`	`X`	`FRE`	`@0 Milieu eau douce @5 26`
C07	`06`	`X`	`ENG`	`@0 Freshwater environment @5 26`
C07	`06`	`X`	`SPA`	`@0 Medio agua dulce @5 26`
C07	`07`	`X`	`FRE`	`@0 Acipenseridae @4 INC @5 32`
C07	`08`	`X`	`FRE`	`@0 Vertebrata @2 NS`
C07	`08`	`X`	`ENG`	`@0 Vertebrata @2 NS`
C07	`08`	`X`	`SPA`	`@0 Vertebrata @2 NS`
N21				`@1 290`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 11-0426955 INIST
ET :	Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon
AU :	KUROBE (T.); KWAK (K. T.); MACCONNELL (E.); MCDOWELL (T. S.); MARDONES (F. O.); HEDRICK (R. P.)
AF :	Department of Medicine and Epidemiology, University of California/Davis, California 95616/Etats-Unis (1 aut., 2 aut., 4 aut., 6 aut.); Center for Animal Disease Modeling and Surveillance (CADMS), School of Veterinary Medicine, University of California/Davis, California 95616/Etats-Unis (5 aut.); US Fish and Wildlife Service, Fish Health Center/Bozeman, Montana 59715/Etats-Unis (3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Diseases of aquatic organisms; ISSN 0177-5103; Coden DAOREO; Allemagne; Da. 2010; Vol. 93; No. 1; Pp. 31-42; Bibl. 1 p.1/4
LA :	Anglais
EA :	The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.
CC :	002A14B04C; 002A15B
FD :	Développement; Réaction chaîne polymérase; Iridovirus; Infection; Captivité; Cours eau; Missouri; Pisces; Acipenser; Scaphirhynchus albus
FG :	Iridoviridae; Virus; Etats-Unis; Amérique du Nord; Amérique; Milieu eau douce; Acipenseridae; Vertebrata
ED :	Development; Polymerase chain reaction; Iridovirus; Infection; Captivity; Stream; Missouri; Pisces
EG :	Iridoviridae; Virus; United States; North America; America; Freshwater environment; Vertebrata
SD :	Desarrollo; Reacción cadena polimerasa; Iridovirus; Infección; Cautividad; Curso agua; Misuri; Pisces
LO :	INIST-21304.354000194047800030
ID :	11-0426955

Links to Exploration step

Pascal:11-0426955

Le document en format XML

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<term>Captivité</term>
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<front><div type="abstract" xml:lang="en">The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.</div>
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<fA14 i1="01"><s1>Department of Medicine and Epidemiology, University of California</s1>
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<fC01 i1="01" l="ENG"><s0>The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A14B04C</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A15B</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Développement</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Development</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Desarrollo</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Réaction chaîne polymérase</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Polymerase chain reaction</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Reacción cadena polimerasa</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Iridovirus</s0>
<s2>NW</s2>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Iridovirus</s0>
<s2>NW</s2>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Iridovirus</s0>
<s2>NW</s2>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Infection</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Infection</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Infección</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Captivité</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Captivity</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Cautividad</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Cours eau</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Stream</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Curso agua</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Missouri</s0>
<s2>NG</s2>
<s5>19</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Missouri</s0>
<s2>NG</s2>
<s5>19</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Misuri</s0>
<s2>NG</s2>
<s5>19</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Pisces</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Pisces</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Pisces</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Acipenser</s0>
<s4>INC</s4>
<s5>64</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Scaphirhynchus albus</s0>
<s4>INC</s4>
<s5>87</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Iridoviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Iridoviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Iridoviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Etats-Unis</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>United States</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Estados Unidos</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Amérique du Nord</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>North America</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>America del norte</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Amérique</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>America</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>America</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Milieu eau douce</s0>
<s5>26</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Freshwater environment</s0>
<s5>26</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Medio agua dulce</s0>
<s5>26</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Acipenseridae</s0>
<s4>INC</s4>
<s5>32</s5>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>290</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 11-0426955 INIST</NO>
<ET>Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon</ET>
<AU>KUROBE (T.); KWAK (K. T.); MACCONNELL (E.); MCDOWELL (T. S.); MARDONES (F. O.); HEDRICK (R. P.)</AU>
<AF>Department of Medicine and Epidemiology, University of California/Davis, California 95616/Etats-Unis (1 aut., 2 aut., 4 aut., 6 aut.); Center for Animal Disease Modeling and Surveillance (CADMS), School of Veterinary Medicine, University of California/Davis, California 95616/Etats-Unis (5 aut.); US Fish and Wildlife Service, Fish Health Center/Bozeman, Montana 59715/Etats-Unis (3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Diseases of aquatic organisms; ISSN 0177-5103; Coden DAOREO; Allemagne; Da. 2010; Vol. 93; No. 1; Pp. 31-42; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.</EA>
<CC>002A14B04C; 002A15B</CC>
<FD>Développement; Réaction chaîne polymérase; Iridovirus; Infection; Captivité; Cours eau; Missouri; Pisces; Acipenser; Scaphirhynchus albus</FD>
<FG>Iridoviridae; Virus; Etats-Unis; Amérique du Nord; Amérique; Milieu eau douce; Acipenseridae; Vertebrata</FG>
<ED>Development; Polymerase chain reaction; Iridovirus; Infection; Captivity; Stream; Missouri; Pisces</ED>
<EG>Iridoviridae; Virus; United States; North America; America; Freshwater environment; Vertebrata</EG>
<SD>Desarrollo; Reacción cadena polimerasa; Iridovirus; Infección; Cautividad; Curso agua; Misuri; Pisces</SD>
<LO>INIST-21304.354000194047800030</LO>
<ID>11-0426955</ID>
</server>
</inist>
</record>

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Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon

Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon

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