Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds
Identifieur interne : 000001 ( PascalFrancis/Corpus ); précédent : 000000; suivant : 000002Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds
Auteurs : Jon A. Doering ; Reza Farmahin ; Steve Wiseman ; Sean W. Kennedy ; John P. Giesy ; Markus HeckerSource :
- Environmental science & technology [ 0013-936X ] ; 2014.
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Abstract
Worldwide, populations of sturgeons are endangered, and it is hypothesized that anthropogenic chemicals, including dioxin-like compounds (DLCs), might be contributing to the observed declines in populations. DLCs elicit their toxic action through activation of the aryl hydrocarbon receptor (AhR), which is believed to regulate most, if not all, adverse effects associated with exposure to these chemicals. Currently, risk assessment of DLCs in fishes uses toxic equivalency factors (TEFs) developed for the World Health Organization (WHO) that are based on studies of embryo-lethality with salmonids. However, there is a lack of knowledge of the sensitivity of sturgeons to DLCs, and it is uncertain whether TEFs developed by the WHO are protective of these fishes. Sturgeons are evolutionarily distinct from salmonids, and the AhRs of sturgeons differ from those of salmonids. Therefore, this study investigated the sensitivity of white sturgeon (Acipenser transmontanus) to DLCs in vitro via the use of luciferase reporter gene assays using COS-7 cells transfected with AhR1 or AhR2 of white sturgeon. Specifically, activation and relative potencies (RePs) of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachloro-dibenzofuran, 2,3,7,8-tetrachloro-dibenzofuran, 3,3',4,4',5-pentachlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,3,3',4,4'-pentachlorobiphenyl were determined for each AhR. It was demonstrated that white sturgeon expresses AhRls and AhR2s that are both activated by DLCs with EC50 values for 2,3,7,8-TCDD that are lower than those of any other AhR of vertebrates tested to date. Both AhRs of white sturgeon had RePs for polychlorinated dibenzofurans more similar to TEFs for birds, while RePs for polychlorinated biphenyls were most similar to TEFs for fishes. Measured concentrations of select DLCs in tissues of white sturgeon from British Columbia, Canada, were used to calculate toxic equivalents (TEQs) by use of TEFs for fishes used by the WHO and TCDD equivalents (TCDD-EQs) via the use of RePs for AhR2 of white sturgeon as determined by transfected COS-7 cells. TCDD-EQs calculated for endangered populations of white sturgeon were approximately 10-fold greater than TEQs and were within ranges known to cause adverse effects in other fishes, including other species of sturgeons. Therefore, TEFs used by the WHO might not adequately protect white sturgeon, illuminating the need for additional investigation into the sensitivity of these fish to DLCs.
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| NO : | PASCAL 15-0037904 INIST |
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| ET : | Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds |
| AU : | DOERING (Jon A.); FARMAHIN (Reza); WISEMAN (Steve); KENNEDY (Sean W.); GIESY (John P.); HECKER (Markus) |
| AF : | Toxicology Graduate Program, University of Saskatchewan/Saskatoon, SK/Canada (1 aut.); Toxicology Centre, University of Saskatchewan/Saskatoon, SK/Canada (1 aut., 3 aut., 5 aut., 6 aut.); Environment Canada, National Wildlife Research Centre/Ottawa, ON/Canada (2 aut., 4 aut.); Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa/Ottawa, ON/Canada (2 aut., 4 aut.); Department of Veterinary Biomedical Sciences, University of Saskatchewan/Saskatoon, SK/Canada (5 aut.); Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong/Kowloon/Hong-Kong (5 aut.); School of Biological Sciences, University of Hong Kong/Hong-Kong (5 aut.); State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University/Nanjing/Chine (5 aut.); School of the Environment and Sustainability, University of Saskatchewan/Saskatoon, SK/Canada (6 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Environmental science & technology; ISSN 0013-936X; Coden ESTHAG; Etats-Unis; Da. 2014; Vol. 48; No. 14; Pp. 8219-8226; Bibl. 57 ref. |
| LA : | Anglais |
| EA : | Worldwide, populations of sturgeons are endangered, and it is hypothesized that anthropogenic chemicals, including dioxin-like compounds (DLCs), might be contributing to the observed declines in populations. DLCs elicit their toxic action through activation of the aryl hydrocarbon receptor (AhR), which is believed to regulate most, if not all, adverse effects associated with exposure to these chemicals. Currently, risk assessment of DLCs in fishes uses toxic equivalency factors (TEFs) developed for the World Health Organization (WHO) that are based on studies of embryo-lethality with salmonids. However, there is a lack of knowledge of the sensitivity of sturgeons to DLCs, and it is uncertain whether TEFs developed by the WHO are protective of these fishes. Sturgeons are evolutionarily distinct from salmonids, and the AhRs of sturgeons differ from those of salmonids. Therefore, this study investigated the sensitivity of white sturgeon (Acipenser transmontanus) to DLCs in vitro via the use of luciferase reporter gene assays using COS-7 cells transfected with AhR1 or AhR2 of white sturgeon. Specifically, activation and relative potencies (RePs) of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachloro-dibenzofuran, 2,3,7,8-tetrachloro-dibenzofuran, 3,3',4,4',5-pentachlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,3,3',4,4'-pentachlorobiphenyl were determined for each AhR. It was demonstrated that white sturgeon expresses AhRls and AhR2s that are both activated by DLCs with EC50 values for 2,3,7,8-TCDD that are lower than those of any other AhR of vertebrates tested to date. Both AhRs of white sturgeon had RePs for polychlorinated dibenzofurans more similar to TEFs for birds, while RePs for polychlorinated biphenyls were most similar to TEFs for fishes. Measured concentrations of select DLCs in tissues of white sturgeon from British Columbia, Canada, were used to calculate toxic equivalents (TEQs) by use of TEFs for fishes used by the WHO and TCDD equivalents (TCDD-EQs) via the use of RePs for AhR2 of white sturgeon as determined by transfected COS-7 cells. TCDD-EQs calculated for endangered populations of white sturgeon were approximately 10-fold greater than TEQs and were within ranges known to cause adverse effects in other fishes, including other species of sturgeons. Therefore, TEFs used by the WHO might not adequately protect white sturgeon, illuminating the need for additional investigation into the sensitivity of these fish to DLCs. |
| CC : | 002A14D05H1 |
| FD : | Hydrocarbure; Récepteur Ah; Pollution eau; Dérivé de la dioxine; Acipenser transmontanus; Toxicité; Milieu eau douce; Evaluation du risque |
| FG : | Pisces; Vertebrata; Composé organique; Polluant organique persistant |
| ED : | Hydrocarbon; Ah receptor; Water pollution; Dioxin derivatives; Acipenser transmontanus; Toxicity; Freshwater environment; Risk evaluation |
| EG : | Pisces; Vertebrata; Organic compounds; Persistent organic pollutant |
| SD : | Hidrocarburo; Receptor Ah; Contaminación agua; Acipenser transmontanus; Toxicidad; Medio agua dulce |
| LO : | INIST-13615.354000504838590670 |
| ID : | 15-0037904 |
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Pascal:15-0037904Le document en format XML
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Doering</name><affiliation><inist:fA14 i1="01"><s1>Toxicology Graduate Program, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="02"><s1>Toxicology Centre, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Farmahin, Reza" sort="Farmahin, Reza" uniqKey="Farmahin R" first="Reza" last="Farmahin">Reza Farmahin</name><affiliation><inist:fA14 i1="03"><s1>Environment Canada, National Wildlife Research Centre</s1><s2>Ottawa, ON</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="04"><s1>Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa</s1><s2>Ottawa, ON</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Wiseman, Steve" sort="Wiseman, Steve" uniqKey="Wiseman S" first="Steve" last="Wiseman">Steve Wiseman</name><affiliation><inist:fA14 i1="02"><s1>Toxicology Centre, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Kennedy, Sean W" sort="Kennedy, Sean W" uniqKey="Kennedy S" first="Sean W." last="Kennedy">Sean W. Kennedy</name><affiliation><inist:fA14 i1="03"><s1>Environment Canada, National Wildlife Research Centre</s1><s2>Ottawa, ON</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="04"><s1>Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa</s1><s2>Ottawa, ON</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Giesy, John P" sort="Giesy, John P" uniqKey="Giesy J" first="John P." last="Giesy">John P. Giesy</name><affiliation><inist:fA14 i1="02"><s1>Toxicology Centre, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="05"><s1>Department of Veterinary Biomedical Sciences, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>5 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="06"><s1>Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>5 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="07"><s1>School of Biological Sciences, University of Hong Kong</s1><s3>HKG</s3><sZ>5 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="08"><s1>State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University</s1><s2>Nanjing</s2><s3>CHN</s3><sZ>5 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Hecker, Markus" sort="Hecker, Markus" uniqKey="Hecker M" first="Markus" last="Hecker">Markus Hecker</name><affiliation><inist:fA14 i1="02"><s1>Toxicology Centre, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="09"><s1>School of the Environment and Sustainability, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>6 aut.</sZ></inist:fA14></affiliation></author></analytic><series><title level="j" type="main">Environmental science & technology</title><title level="j" type="abbreviated">Environ. sci. technol.</title><idno type="ISSN">0013-936X</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Environmental science & technology</title><title level="j" type="abbreviated">Environ. sci. technol.</title><idno type="ISSN">0013-936X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term><term>Ah receptor</term><term>Dioxin derivatives</term><term>Freshwater environment</term><term>Hydrocarbon</term><term>Risk evaluation</term><term>Toxicity</term><term>Water pollution</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Hydrocarbure</term><term>Récepteur Ah</term><term>Pollution eau</term><term>Dérivé de la dioxine</term><term>Acipenser transmontanus</term><term>Toxicité</term><term>Milieu eau douce</term><term>Evaluation du risque</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Worldwide, populations of sturgeons are endangered, and it is hypothesized that anthropogenic chemicals, including dioxin-like compounds (DLCs), might be contributing to the observed declines in populations. DLCs elicit their toxic action through activation of the aryl hydrocarbon receptor (AhR), which is believed to regulate most, if not all, adverse effects associated with exposure to these chemicals. Currently, risk assessment of DLCs in fishes uses toxic equivalency factors (TEFs) developed for the World Health Organization (WHO) that are based on studies of embryo-lethality with salmonids. However, there is a lack of knowledge of the sensitivity of sturgeons to DLCs, and it is uncertain whether TEFs developed by the WHO are protective of these fishes. Sturgeons are evolutionarily distinct from salmonids, and the AhRs of sturgeons differ from those of salmonids. Therefore, this study investigated the sensitivity of white sturgeon (Acipenser transmontanus) to DLCs in vitro via the use of luciferase reporter gene assays using COS-7 cells transfected with AhR1 or AhR2 of white sturgeon. Specifically, activation and relative potencies (RePs) of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachloro-dibenzofuran, 2,3,7,8-tetrachloro-dibenzofuran, 3,3',4,4',5-pentachlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,3,3',4,4'-pentachlorobiphenyl were determined for each AhR. It was demonstrated that white sturgeon expresses AhRls and AhR2s that are both activated by DLCs with EC<sub>50</sub> values for 2,3,7,8-TCDD that are lower than those of any other AhR of vertebrates tested to date. Both AhRs of white sturgeon had RePs for polychlorinated dibenzofurans more similar to TEFs for birds, while RePs for polychlorinated biphenyls were most similar to TEFs for fishes. Measured concentrations of select DLCs in tissues of white sturgeon from British Columbia, Canada, were used to calculate toxic equivalents (TEQs) by use of TEFs for fishes used by the WHO and TCDD equivalents (TCDD-EQs) via the use of RePs for AhR2 of white sturgeon as determined by transfected COS-7 cells. TCDD-EQs calculated for endangered populations of white sturgeon were approximately 10-fold greater than TEQs and were within ranges known to cause adverse effects in other fishes, including other species of sturgeons. Therefore, TEFs used by the WHO might not adequately protect white sturgeon, illuminating the need for additional investigation into the sensitivity of these fish to DLCs.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0013-936X</s0></fA01><fA02 i1="01"><s0>ESTHAG</s0></fA02><fA03 i2="1"><s0>Environ. sci. technol.</s0></fA03><fA05><s2>48</s2></fA05><fA06><s2>14</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds</s1></fA08><fA11 i1="01" i2="1"><s1>DOERING (Jon A.)</s1></fA11><fA11 i1="02" i2="1"><s1>FARMAHIN (Reza)</s1></fA11><fA11 i1="03" i2="1"><s1>WISEMAN (Steve)</s1></fA11><fA11 i1="04" i2="1"><s1>KENNEDY (Sean W.)</s1></fA11><fA11 i1="05" i2="1"><s1>GIESY (John P.)</s1></fA11><fA11 i1="06" i2="1"><s1>HECKER (Markus)</s1></fA11><fA14 i1="01"><s1>Toxicology Graduate Program, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ></fA14><fA14 i1="02"><s1>Toxicology Centre, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="03"><s1>Environment Canada, National Wildlife Research Centre</s1><s2>Ottawa, ON</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="04"><s1>Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa</s1><s2>Ottawa, ON</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="05"><s1>Department of Veterinary Biomedical Sciences, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="06"><s1>Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>5 aut.</sZ></fA14><fA14 i1="07"><s1>School of Biological Sciences, University of Hong Kong</s1><s3>HKG</s3><sZ>5 aut.</sZ></fA14><fA14 i1="08"><s1>State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University</s1><s2>Nanjing</s2><s3>CHN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="09"><s1>School of the Environment and Sustainability, University of Saskatchewan</s1><s2>Saskatoon, SK</s2><s3>CAN</s3><sZ>6 aut.</sZ></fA14><fA20><s1>8219-8226</s1></fA20><fA21><s1>2014</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>13615</s2><s5>354000504838590670</s5></fA43><fA44><s0>0000</s0><s1>© 2015 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>57 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>15-0037904</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Environmental science & technology</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Worldwide, populations of sturgeons are endangered, and it is hypothesized that anthropogenic chemicals, including dioxin-like compounds (DLCs), might be contributing to the observed declines in populations. DLCs elicit their toxic action through activation of the aryl hydrocarbon receptor (AhR), which is believed to regulate most, if not all, adverse effects associated with exposure to these chemicals. Currently, risk assessment of DLCs in fishes uses toxic equivalency factors (TEFs) developed for the World Health Organization (WHO) that are based on studies of embryo-lethality with salmonids. However, there is a lack of knowledge of the sensitivity of sturgeons to DLCs, and it is uncertain whether TEFs developed by the WHO are protective of these fishes. Sturgeons are evolutionarily distinct from salmonids, and the AhRs of sturgeons differ from those of salmonids. Therefore, this study investigated the sensitivity of white sturgeon (Acipenser transmontanus) to DLCs in vitro via the use of luciferase reporter gene assays using COS-7 cells transfected with AhR1 or AhR2 of white sturgeon. Specifically, activation and relative potencies (RePs) of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachloro-dibenzofuran, 2,3,7,8-tetrachloro-dibenzofuran, 3,3',4,4',5-pentachlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,3,3',4,4'-pentachlorobiphenyl were determined for each AhR. It was demonstrated that white sturgeon expresses AhRls and AhR2s that are both activated by DLCs with EC<sub>50</sub> values for 2,3,7,8-TCDD that are lower than those of any other AhR of vertebrates tested to date. Both AhRs of white sturgeon had RePs for polychlorinated dibenzofurans more similar to TEFs for birds, while RePs for polychlorinated biphenyls were most similar to TEFs for fishes. Measured concentrations of select DLCs in tissues of white sturgeon from British Columbia, Canada, were used to calculate toxic equivalents (TEQs) by use of TEFs for fishes used by the WHO and TCDD equivalents (TCDD-EQs) via the use of RePs for AhR2 of white sturgeon as determined by transfected COS-7 cells. TCDD-EQs calculated for endangered populations of white sturgeon were approximately 10-fold greater than TEQs and were within ranges known to cause adverse effects in other fishes, including other species of sturgeons. Therefore, TEFs used by the WHO might not adequately protect white sturgeon, illuminating the need for additional investigation into the sensitivity of these fish to DLCs.</s0></fC01><fC02 i1="01" i2="X"><s0>002A14D05H1</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Hydrocarbure</s0><s2>FX</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Hydrocarbon</s0><s2>FX</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Hidrocarburo</s0><s2>FX</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Récepteur Ah</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Ah receptor</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Receptor Ah</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Pollution eau</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Water pollution</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Contaminación agua</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Dérivé de la dioxine</s0><s2>FR</s2><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Dioxin derivatives</s0><s2>FR</s2><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Acipenser transmontanus</s0><s2>NS</s2><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Acipenser transmontanus</s0><s2>NS</s2><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Acipenser transmontanus</s0><s2>NS</s2><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Toxicité</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Toxicity</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Toxicidad</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Milieu eau douce</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Freshwater environment</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Medio agua dulce</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Evaluation du risque</s0><s4>CD</s4><s5>97</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Risk evaluation</s0><s4>CD</s4><s5>97</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Pisces</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Pisces</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Pisces</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Composé organique</s0><s2>NA</s2><s5>61</s5></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Organic compounds</s0><s2>NA</s2><s5>61</s5></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Compuesto orgánico</s0><s2>NA</s2><s5>61</s5></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Polluant organique persistant</s0><s5>62</s5></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Persistent organic pollutant</s0><s5>62</s5></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Contaminante organico persistente</s0><s5>62</s5></fC07><fN21><s1>068</s1></fN21></pA></standard><server><NO>PASCAL 15-0037904 INIST</NO><ET>Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds</ET><AU>DOERING (Jon A.); FARMAHIN (Reza); WISEMAN (Steve); KENNEDY (Sean W.); GIESY (John P.); HECKER (Markus)</AU><AF>Toxicology Graduate Program, University of Saskatchewan/Saskatoon, SK/Canada (1 aut.); Toxicology Centre, University of Saskatchewan/Saskatoon, SK/Canada (1 aut., 3 aut., 5 aut., 6 aut.); Environment Canada, National Wildlife Research Centre/Ottawa, ON/Canada (2 aut., 4 aut.); Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa/Ottawa, ON/Canada (2 aut., 4 aut.); Department of Veterinary Biomedical Sciences, University of Saskatchewan/Saskatoon, SK/Canada (5 aut.); Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong/Kowloon/Hong-Kong (5 aut.); School of Biological Sciences, University of Hong Kong/Hong-Kong (5 aut.); State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University/Nanjing/Chine (5 aut.); School of the Environment and Sustainability, University of Saskatchewan/Saskatoon, SK/Canada (6 aut.)</AF><DT>Publication en série; Niveau analytique</DT><SO>Environmental science & technology; ISSN 0013-936X; Coden ESTHAG; Etats-Unis; Da. 2014; Vol. 48; No. 14; Pp. 8219-8226; Bibl. 57 ref.</SO><LA>Anglais</LA><EA>Worldwide, populations of sturgeons are endangered, and it is hypothesized that anthropogenic chemicals, including dioxin-like compounds (DLCs), might be contributing to the observed declines in populations. DLCs elicit their toxic action through activation of the aryl hydrocarbon receptor (AhR), which is believed to regulate most, if not all, adverse effects associated with exposure to these chemicals. Currently, risk assessment of DLCs in fishes uses toxic equivalency factors (TEFs) developed for the World Health Organization (WHO) that are based on studies of embryo-lethality with salmonids. However, there is a lack of knowledge of the sensitivity of sturgeons to DLCs, and it is uncertain whether TEFs developed by the WHO are protective of these fishes. Sturgeons are evolutionarily distinct from salmonids, and the AhRs of sturgeons differ from those of salmonids. Therefore, this study investigated the sensitivity of white sturgeon (Acipenser transmontanus) to DLCs in vitro via the use of luciferase reporter gene assays using COS-7 cells transfected with AhR1 or AhR2 of white sturgeon. Specifically, activation and relative potencies (RePs) of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachloro-dibenzofuran, 2,3,7,8-tetrachloro-dibenzofuran, 3,3',4,4',5-pentachlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,3,3',4,4'-pentachlorobiphenyl were determined for each AhR. It was demonstrated that white sturgeon expresses AhRls and AhR2s that are both activated by DLCs with EC<sub>50</sub> values for 2,3,7,8-TCDD that are lower than those of any other AhR of vertebrates tested to date. Both AhRs of white sturgeon had RePs for polychlorinated dibenzofurans more similar to TEFs for birds, while RePs for polychlorinated biphenyls were most similar to TEFs for fishes. Measured concentrations of select DLCs in tissues of white sturgeon from British Columbia, Canada, were used to calculate toxic equivalents (TEQs) by use of TEFs for fishes used by the WHO and TCDD equivalents (TCDD-EQs) via the use of RePs for AhR2 of white sturgeon as determined by transfected COS-7 cells. TCDD-EQs calculated for endangered populations of white sturgeon were approximately 10-fold greater than TEQs and were within ranges known to cause adverse effects in other fishes, including other species of sturgeons. Therefore, TEFs used by the WHO might not adequately protect white sturgeon, illuminating the need for additional investigation into the sensitivity of these fish to DLCs.</EA><CC>002A14D05H1</CC><FD>Hydrocarbure; Récepteur Ah; Pollution eau; Dérivé de la dioxine; Acipenser transmontanus; Toxicité; Milieu eau douce; Evaluation du risque</FD><FG>Pisces; Vertebrata; Composé organique; Polluant organique persistant</FG><ED>Hydrocarbon; Ah receptor; Water pollution; Dioxin derivatives; Acipenser transmontanus; Toxicity; Freshwater environment; Risk evaluation</ED><EG>Pisces; Vertebrata; Organic compounds; Persistent organic pollutant</EG><SD>Hidrocarburo; Receptor Ah; Contaminación agua; Acipenser transmontanus; Toxicidad; Medio agua dulce</SD><LO>INIST-13615.354000504838590670</LO><ID>15-0037904</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
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