Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: molecular cloning and distribution of the mRNAs encoding two somatostatin precursors.
Identifieur interne : 000044 ( Ncbi/Merge ); précédent : 000043; suivant : 000045Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: molecular cloning and distribution of the mRNAs encoding two somatostatin precursors.
Auteurs : Michele Trabucchi [France] ; Hervé Tostivint ; Isabelle Lihrmann ; Cristina Sollars ; Mauro Vallarino ; Robert M. Dores ; Hubert VaudrySource :
- The Journal of comparative neurology [ 0021-9967 ] ; 2002.
English descriptors
- KwdEn :
- Amino Acid Sequence (genetics), Animals, Brain Chemistry (genetics), Cloning, Molecular (methods), Female, Fishes, Gene Expression Regulation, Male, Molecular Sequence Data, Multifactorial Inheritance (genetics), RNA, Messenger (biosynthesis), RNA, Messenger (isolation & purification), Somatostatin (biosynthesis), Somatostatin (genetics), Somatostatin (isolation & purification).
- MESH :
- chemical , biosynthesis : RNA, Messenger, Somatostatin.
- genetics : Amino Acid Sequence, Brain Chemistry, Multifactorial Inheritance, Somatostatin.
- chemical , isolation & purification : RNA, Messenger, Somatostatin.
- methods : Cloning, Molecular.
- Animals, Female, Fishes, Gene Expression Regulation, Male, Molecular Sequence Data.
Abstract
The sequence of somatostatin-14 (SS1) has been strongly preserved throughout the evolution of vertebrates from agnathans to mammals. In Acipenseridae (sturgeons), two isoforms of somatostatin have been characterized to date: somatostatin-14 has been identified from the gastrointestinal tract of the pallid sturgeon Scaphirhynchus albus and [Pro(2)]somatostatin-14 has been identified from the pituitary of the Russian sturgeon Acipenser gueldenstaedti. In the present study, we report the cloning of two distinct somatostatin cDNAs from the brain of the sturgeon Acipenser transmontanus. One of the cDNAs encodes a 116-amino acid protein (PSS1) that contains the SS1 sequence at its C-terminal extremity and, thus, is clearly orthologous to other vertebrate PSS1. The other cDNA encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]somatostatin-14 at its C-terminal extremity. This second precursor exhibits more than 67% identity with the recently characterized lungfish PSS2 and goldfish PSS2. Reverse transcriptase-polymerase chain reaction analysis revealed that PSS1 is expressed in the central nervous system, the pancreas and the gut, whereas PSS2 is found in the central nervous system but not in the digestive system. In situ hybridization histochemistry showed that the PSS1 and PSS2 genes are differently expressed in numerous regions of the sturgeon brain. Interestingly, PSS1 and PSS2 mRNAs are present in the hypothalamus suggesting that, in sturgeon, both SS1 and SS2 may play hypophysiotropic functions. The PSS2 mRNA but not the PSS1 mRNA was found in the intermediate lobe of the pituitary. The present data demonstrate that two somatostatin genes are expressed in the sturgeon brain: one precursor generates somatostatin-14 and the other one gives rise to a [Pro(2)]somatostatin-14 variant, which is orthologous to goldfish, lungfish, and frog SS2.
PubMed: 11807842
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Dores</name></author><author><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name></author></analytic><series><title level="j">The Journal of comparative neurology</title><idno type="ISSN">0021-9967</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (genetics)</term><term>Animals</term><term>Brain Chemistry (genetics)</term><term>Cloning, Molecular (methods)</term><term>Female</term><term>Fishes</term><term>Gene Expression Regulation</term><term>Male</term><term>Molecular Sequence Data</term><term>Multifactorial Inheritance (genetics)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (isolation & purification)</term><term>Somatostatin (biosynthesis)</term><term>Somatostatin (genetics)</term><term>Somatostatin (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term><term>Somatostatin</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amino Acid Sequence</term><term>Brain Chemistry</term><term>Multifactorial Inheritance</term><term>Somatostatin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Messenger</term><term>Somatostatin</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cloning, Molecular</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Fishes</term><term>Gene Expression Regulation</term><term>Male</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The sequence of somatostatin-14 (SS1) has been strongly preserved throughout the evolution of vertebrates from agnathans to mammals. In Acipenseridae (sturgeons), two isoforms of somatostatin have been characterized to date: somatostatin-14 has been identified from the gastrointestinal tract of the pallid sturgeon Scaphirhynchus albus and [Pro(2)]somatostatin-14 has been identified from the pituitary of the Russian sturgeon Acipenser gueldenstaedti. In the present study, we report the cloning of two distinct somatostatin cDNAs from the brain of the sturgeon Acipenser transmontanus. One of the cDNAs encodes a 116-amino acid protein (PSS1) that contains the SS1 sequence at its C-terminal extremity and, thus, is clearly orthologous to other vertebrate PSS1. The other cDNA encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]somatostatin-14 at its C-terminal extremity. This second precursor exhibits more than 67% identity with the recently characterized lungfish PSS2 and goldfish PSS2. Reverse transcriptase-polymerase chain reaction analysis revealed that PSS1 is expressed in the central nervous system, the pancreas and the gut, whereas PSS2 is found in the central nervous system but not in the digestive system. In situ hybridization histochemistry showed that the PSS1 and PSS2 genes are differently expressed in numerous regions of the sturgeon brain. Interestingly, PSS1 and PSS2 mRNAs are present in the hypothalamus suggesting that, in sturgeon, both SS1 and SS2 may play hypophysiotropic functions. The PSS2 mRNA but not the PSS1 mRNA was found in the intermediate lobe of the pituitary. The present data demonstrate that two somatostatin genes are expressed in the sturgeon brain: one precursor generates somatostatin-14 and the other one gives rise to a [Pro(2)]somatostatin-14 variant, which is orthologous to goldfish, lungfish, and frog SS2.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11807842</PMID><DateCreated><Year>2002</Year><Month>01</Month><Day>24</Day></DateCreated><DateCompleted><Year>2002</Year><Month>03</Month><Day>13</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9967</ISSN><JournalIssue CitedMedium="Print"><Volume>443</Volume><Issue>4</Issue><PubDate><Year>2002</Year><Month>Feb</Month><Day>18</Day></PubDate></JournalIssue><Title>The Journal of comparative neurology</Title><ISOAbbreviation>J. Comp. Neurol.</ISOAbbreviation></Journal><ArticleTitle>Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: molecular cloning and distribution of the mRNAs encoding two somatostatin precursors.</ArticleTitle><Pagination><MedlinePgn>332-45</MedlinePgn></Pagination><Abstract><AbstractText>The sequence of somatostatin-14 (SS1) has been strongly preserved throughout the evolution of vertebrates from agnathans to mammals. In Acipenseridae (sturgeons), two isoforms of somatostatin have been characterized to date: somatostatin-14 has been identified from the gastrointestinal tract of the pallid sturgeon Scaphirhynchus albus and [Pro(2)]somatostatin-14 has been identified from the pituitary of the Russian sturgeon Acipenser gueldenstaedti. In the present study, we report the cloning of two distinct somatostatin cDNAs from the brain of the sturgeon Acipenser transmontanus. One of the cDNAs encodes a 116-amino acid protein (PSS1) that contains the SS1 sequence at its C-terminal extremity and, thus, is clearly orthologous to other vertebrate PSS1. The other cDNA encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]somatostatin-14 at its C-terminal extremity. This second precursor exhibits more than 67% identity with the recently characterized lungfish PSS2 and goldfish PSS2. Reverse transcriptase-polymerase chain reaction analysis revealed that PSS1 is expressed in the central nervous system, the pancreas and the gut, whereas PSS2 is found in the central nervous system but not in the digestive system. In situ hybridization histochemistry showed that the PSS1 and PSS2 genes are differently expressed in numerous regions of the sturgeon brain. Interestingly, PSS1 and PSS2 mRNAs are present in the hypothalamus suggesting that, in sturgeon, both SS1 and SS2 may play hypophysiotropic functions. The PSS2 mRNA but not the PSS1 mRNA was found in the intermediate lobe of the pituitary. The present data demonstrate that two somatostatin genes are expressed in the sturgeon brain: one precursor generates somatostatin-14 and the other one gives rise to a [Pro(2)]somatostatin-14 variant, which is orthologous to goldfish, lungfish, and frog SS2.</AbstractText><CopyrightInformation>Copyright 2002 Wiley-Liss, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Trabucchi</LastName><ForeName>Michele</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U-413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tostivint</LastName><ForeName>Hervé</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lihrmann</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Sollars</LastName><ForeName>Cristina</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Vallarino</LastName><ForeName>Mauro</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Dores</LastName><ForeName>Robert M</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Vaudry</LastName><ForeName>Hubert</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AF395849</AccessionNumber><AccessionNumber>AF395850</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, 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Messenger</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013004" MajorTopicYN="N">Somatostatin</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>1</Month><Day>25</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>3</Month><Day>14</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>1</Month><Day>25</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11807842</ArticleId><ArticleId IdType="pii">10.1002/cne.10126</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Mont-Saint-Aignan</li></settlement></list><tree><noCountry><name sortKey="Dores, Robert M" sort="Dores, Robert M" uniqKey="Dores R" first="Robert M" last="Dores">Robert M. Dores</name><name sortKey="Lihrmann, Isabelle" sort="Lihrmann, Isabelle" uniqKey="Lihrmann I" first="Isabelle" last="Lihrmann">Isabelle Lihrmann</name><name sortKey="Sollars, Cristina" sort="Sollars, Cristina" uniqKey="Sollars C" first="Cristina" last="Sollars">Cristina Sollars</name><name sortKey="Tostivint, Herve" sort="Tostivint, Herve" uniqKey="Tostivint H" first="Hervé" last="Tostivint">Hervé Tostivint</name><name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Trabucchi, Michele" sort="Trabucchi, Michele" uniqKey="Trabucchi M" first="Michele" last="Trabucchi">Michele Trabucchi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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