Immunohistochemical distribution of cytochrome P4501A in larvae and fingerlings of the Siberian sturgeon, Acipenser baeri.
Identifieur interne : 000037 ( Ncbi/Merge ); précédent : 000036; suivant : 000038Immunohistochemical distribution of cytochrome P4501A in larvae and fingerlings of the Siberian sturgeon, Acipenser baeri.
Auteurs : C. Sarasquete [Espagne] ; J B Ortiz ; Enric Gisbert [Espagne]Source :
- The Histochemical journal [ 0018-2214 ] ; 2001.
English descriptors
- KwdEn :
- Animals, Cytochrome P-450 Enzyme System (metabolism), Digestive System (embryology), Digestive System (growth & development), Female, Fishes (growth & development), Fishes (metabolism), Immunohistochemistry, Larva (enzymology), Larva (growth & development), Nervous System (growth & development), Nervous System (metabolism), Tissue Distribution.
- MESH :
- chemical , metabolism : Cytochrome P-450 Enzyme System.
- embryology : Digestive System.
- enzymology : Larva.
- growth & development : Digestive System, Fishes, Larva, Nervous System.
- metabolism : Fishes, Nervous System.
- Animals, Female, Immunohistochemistry, Tissue Distribution.
Abstract
In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYPIA), from hatching until 30 days posthatching in developing Siberian sturgeon, Acipenser baeri. For this purpose, a polyclonal (BN-1) antiserum developed against a conservative sequence of piscine CYP1A and a monoclonal (C10-7) antiserum directed against cod CYP1A were used on paraffin-embedded samples. From hatching onwards, distinct CYP1A immunoreactivity was distinctly observed in the following tissues and cells: envelope of oil droplets, matrix and syncytium of the yolk-sac, sinusoids, biliary epithelial cells and hepatocytes. In the digestive tract, buccopharyngeal, oesophageal, gastric and intestinal epithelia, as well as the cytoplasm and brush border of enterocytes were CYP1A-positive. Interestingly, gastric glands and melanin-plug present within lumen of the digestive system were strongly immunoreactive. Kidney (epithelia of renal tubules), gills (pillar and endothelial cells), skin (epithelial cells), muscle fibres of heart and eye (retina) were positive. In brain, we observed a strong CYP1A staining in the developing telencephalon and especially in olfactory system, as well as in those nerve fibres running ventrally toward the posterior brain. A strong CYP1A staining was observed in vascular endothelia of all organs/tissues, especially in the liver. In general, the intensity of CYP1A immunostaining increased during larval development, suggesting besides its known metabolic function (endogenous and/or exogenous), a possible participation of this heme-protein in control of cell division, regulation of growth and differentiation.
PubMed: 11432638
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Sarasquete</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencias Marinas de Andalucía, CSIC, Puerto Real, Cádiz, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Ciencias Marinas de Andalucía, CSIC, Puerto Real, Cádiz</wicri:regionArea><wicri:noRegion>Cádiz</wicri:noRegion></affiliation></author><author><name sortKey="Ortiz, J B" sort="Ortiz, J B" uniqKey="Ortiz J" first="J B" last="Ortiz">J B Ortiz</name></author><author><name sortKey="Gisbert, E" sort="Gisbert, E" uniqKey="Gisbert E" first="E" last="Gisbert">Enric Gisbert</name><affiliation><country>Espagne</country><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName><orgName type="university">Université de Barcelone</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11432638</idno><idno type="pmid">11432638</idno><idno type="wicri:Area/PubMed/Corpus">000629</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000629</idno><idno type="wicri:Area/PubMed/Curation">000629</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000629</idno><idno type="wicri:Area/PubMed/Checkpoint">000629</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000629</idno><idno type="wicri:Area/Ncbi/Merge">000037</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunohistochemical distribution of cytochrome P4501A in larvae and fingerlings of the Siberian sturgeon, Acipenser baeri.</title><author><name sortKey="Sarasquete, C" sort="Sarasquete, C" uniqKey="Sarasquete C" first="C" last="Sarasquete">C. Sarasquete</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencias Marinas de Andalucía, CSIC, Puerto Real, Cádiz, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Ciencias Marinas de Andalucía, CSIC, Puerto Real, Cádiz</wicri:regionArea><wicri:noRegion>Cádiz</wicri:noRegion></affiliation></author><author><name sortKey="Ortiz, J B" sort="Ortiz, J B" uniqKey="Ortiz J" first="J B" last="Ortiz">J B Ortiz</name></author><author><name sortKey="Gisbert, E" sort="Gisbert, E" uniqKey="Gisbert E" first="E" last="Gisbert">Enric Gisbert</name><affiliation><country>Espagne</country><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName><orgName type="university">Université de Barcelone</orgName></affiliation></author></analytic><series><title level="j">The Histochemical journal</title><idno type="ISSN">0018-2214</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cytochrome P-450 Enzyme System (metabolism)</term><term>Digestive System (embryology)</term><term>Digestive System (growth & development)</term><term>Female</term><term>Fishes (growth & development)</term><term>Fishes (metabolism)</term><term>Immunohistochemistry</term><term>Larva (enzymology)</term><term>Larva (growth & development)</term><term>Nervous System (growth & development)</term><term>Nervous System (metabolism)</term><term>Tissue Distribution</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Digestive System</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Larva</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Digestive System</term><term>Fishes</term><term>Larva</term><term>Nervous System</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Nervous System</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Immunohistochemistry</term><term>Tissue Distribution</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYPIA), from hatching until 30 days posthatching in developing Siberian sturgeon, Acipenser baeri. For this purpose, a polyclonal (BN-1) antiserum developed against a conservative sequence of piscine CYP1A and a monoclonal (C10-7) antiserum directed against cod CYP1A were used on paraffin-embedded samples. From hatching onwards, distinct CYP1A immunoreactivity was distinctly observed in the following tissues and cells: envelope of oil droplets, matrix and syncytium of the yolk-sac, sinusoids, biliary epithelial cells and hepatocytes. In the digestive tract, buccopharyngeal, oesophageal, gastric and intestinal epithelia, as well as the cytoplasm and brush border of enterocytes were CYP1A-positive. Interestingly, gastric glands and melanin-plug present within lumen of the digestive system were strongly immunoreactive. Kidney (epithelia of renal tubules), gills (pillar and endothelial cells), skin (epithelial cells), muscle fibres of heart and eye (retina) were positive. In brain, we observed a strong CYP1A staining in the developing telencephalon and especially in olfactory system, as well as in those nerve fibres running ventrally toward the posterior brain. A strong CYP1A staining was observed in vascular endothelia of all organs/tissues, especially in the liver. In general, the intensity of CYP1A immunostaining increased during larval development, suggesting besides its known metabolic function (endogenous and/or exogenous), a possible participation of this heme-protein in control of cell division, regulation of growth and differentiation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11432638</PMID><DateCreated><Year>2001</Year><Month>07</Month><Day>02</Day></DateCreated><DateCompleted><Year>2001</Year><Month>12</Month><Day>07</Day></DateCompleted><DateRevised><Year>2003</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0018-2214</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>2</Issue><PubDate><Year>2001</Year><Month>Feb</Month></PubDate></JournalIssue><Title>The Histochemical journal</Title><ISOAbbreviation>Histochem. J.</ISOAbbreviation></Journal><ArticleTitle>Immunohistochemical distribution of cytochrome P4501A in larvae and fingerlings of the Siberian sturgeon, Acipenser baeri.</ArticleTitle><Pagination><MedlinePgn>101-10</MedlinePgn></Pagination><Abstract><AbstractText>In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYPIA), from hatching until 30 days posthatching in developing Siberian sturgeon, Acipenser baeri. For this purpose, a polyclonal (BN-1) antiserum developed against a conservative sequence of piscine CYP1A and a monoclonal (C10-7) antiserum directed against cod CYP1A were used on paraffin-embedded samples. From hatching onwards, distinct CYP1A immunoreactivity was distinctly observed in the following tissues and cells: envelope of oil droplets, matrix and syncytium of the yolk-sac, sinusoids, biliary epithelial cells and hepatocytes. In the digestive tract, buccopharyngeal, oesophageal, gastric and intestinal epithelia, as well as the cytoplasm and brush border of enterocytes were CYP1A-positive. Interestingly, gastric glands and melanin-plug present within lumen of the digestive system were strongly immunoreactive. Kidney (epithelia of renal tubules), gills (pillar and endothelial cells), skin (epithelial cells), muscle fibres of heart and eye (retina) were positive. In brain, we observed a strong CYP1A staining in the developing telencephalon and especially in olfactory system, as well as in those nerve fibres running ventrally toward the posterior brain. A strong CYP1A staining was observed in vascular endothelia of all organs/tissues, especially in the liver. 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Sarasquete</name></noRegion><name sortKey="Gisbert, E" sort="Gisbert, E" uniqKey="Gisbert E" first="E" last="Gisbert">Enric Gisbert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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