Fundulus heteroclitus vitellogenin: the deduced primary structure of a piscine precursor to noncrystalline, liquid-phase yolk protein.
Identifieur interne : 000846 ( Ncbi/Curation ); précédent : 000845; suivant : 000847Fundulus heteroclitus vitellogenin: the deduced primary structure of a piscine precursor to noncrystalline, liquid-phase yolk protein.
Auteurs : G J Lafleur [États-Unis] ; B M Byrne ; J. Kanungo ; L D Nelson ; R M Greenberg ; R A WallaceSource :
- Journal of molecular evolution [ 0022-2844 ] ; 1995.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Biological Evolution, Chickens (genetics), Codon (genetics), DNA, Complementary, Egg Proteins (chemistry), Egg Proteins (genetics), Female, Killifishes (genetics), Lampreys (genetics), Liver (metabolism), Male, Molecular Sequence Data, Oncorhynchus mykiss (genetics), Open Reading Frames, Protein Precursors (biosynthesis), Protein Precursors (genetics), Sequence Homology, Amino Acid, Vitellogenins (chemistry), Vitellogenins (genetics), Xenopus laevis (genetics).
- MESH :
- chemical , biosynthesis : Protein Precursors.
- chemical , chemistry : Egg Proteins, Vitellogenins.
- chemical , genetics : Codon, Egg Proteins, Protein Precursors, Vitellogenins.
- genetics : Chickens, Killifishes, Lampreys, Oncorhynchus mykiss, Xenopus laevis.
- metabolism : Liver.
- Amino Acid Sequence, Animals, Base Sequence, Biological Evolution, DNA, Complementary, Female, Male, Molecular Sequence Data, Open Reading Frames, Sequence Homology, Amino Acid.
Abstract
We have cloned and sequenced a cDNA encoding a vitellogenin (Vtg) from the mummichog, Fundulus heteroclitus, an estuarine teleost. We constructed a liver cDNA library against RNA from estrogen-treated male mummichogs. Five overlapping cDNA clones totalling 5,197 bp were isolated through a combination of degenerate oligonucleotide probing of the library and PCR. The cDNA sequence contains a 5,112 bp open reading frame. The predicted primary structure of the deduced 1,704-amino-acid protein is 30-40% identical to other documented chordate Vtgs, establishing this Vtg as a member of the ancient Vtg gene family. Of the previously reported chordate Vtg sequences (Xenopus laevis, Gallus domesticus, Ichthyomyzon unicuspis, and Acipenser transmontanus), all four act as precursor proteins to a yolk which is eventually rendered insoluble under physiological conditions, either as crystalline platelets or as noncrystalline granules. The yolk of F. heteroclitus, on the other hand, remains in a soluble state throughout oocyte growth. The putative F. heteroclitus Vtg contains a polyserine region with a relative serine composition that is 10-20% higher than that observed for the other Vtgs. The trinucleotide repeats encoding the characteristic polyserine tracts of the phosvitin region follow a previously reported trend: TCX codons on the 5' end and AGY codons toward the 3' end. Whether the difference in Vtg primary structure between F. heteroclitus and that of other chordates is responsible for the differences in yolk structure remains to be elucidated. As the first complete teleost Vtg to be reported, these data will aid in designing nucleotide and immunological probes for detecting Vtg as a reproductive status indicator in F. heteroclitus and other piscine species.
PubMed: 7563139
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pubmed:7563139Le document en format XML
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Kanungo</name></author><author><name sortKey="Nelson, L D" sort="Nelson, L D" uniqKey="Nelson L" first="L D" last="Nelson">L D Nelson</name></author><author><name sortKey="Greenberg, R M" sort="Greenberg, R M" uniqKey="Greenberg R" first="R M" last="Greenberg">R M Greenberg</name></author><author><name sortKey="Wallace, R A" sort="Wallace, R A" uniqKey="Wallace R" first="R A" last="Wallace">R A Wallace</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:7563139</idno><idno type="pmid">7563139</idno><idno type="wicri:Area/PubMed/Corpus">000694</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000694</idno><idno type="wicri:Area/PubMed/Curation">000694</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000694</idno><idno type="wicri:Area/PubMed/Checkpoint">000694</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000694</idno><idno type="wicri:Area/Ncbi/Merge">000846</idno><idno type="wicri:Area/Ncbi/Curation">000846</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fundulus heteroclitus vitellogenin: the deduced primary structure of a piscine precursor to noncrystalline, liquid-phase yolk protein.</title><author><name sortKey="Lafleur, G J" sort="Lafleur, G J" uniqKey="Lafleur G" first="G J" last="Lafleur">G J Lafleur</name><affiliation wicri:level="1"><nlm:affiliation>Whitney Laboratory, University of Florida, Marineland 32086, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Whitney Laboratory, University of Florida, Marineland 32086</wicri:regionArea><wicri:noRegion>Marineland 32086</wicri:noRegion></affiliation></author><author><name sortKey="Byrne, B M" sort="Byrne, B M" uniqKey="Byrne B" first="B M" last="Byrne">B M Byrne</name></author><author><name sortKey="Kanungo, J" sort="Kanungo, J" uniqKey="Kanungo J" first="J" last="Kanungo">J. Kanungo</name></author><author><name sortKey="Nelson, L D" sort="Nelson, L D" uniqKey="Nelson L" first="L D" last="Nelson">L D Nelson</name></author><author><name sortKey="Greenberg, R M" sort="Greenberg, R M" uniqKey="Greenberg R" first="R M" last="Greenberg">R M Greenberg</name></author><author><name sortKey="Wallace, R A" sort="Wallace, R A" uniqKey="Wallace R" first="R A" last="Wallace">R A Wallace</name></author></analytic><series><title level="j">Journal of molecular evolution</title><idno type="ISSN">0022-2844</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Biological Evolution</term><term>Chickens (genetics)</term><term>Codon (genetics)</term><term>DNA, Complementary</term><term>Egg Proteins (chemistry)</term><term>Egg Proteins (genetics)</term><term>Female</term><term>Killifishes (genetics)</term><term>Lampreys (genetics)</term><term>Liver (metabolism)</term><term>Male</term><term>Molecular Sequence Data</term><term>Oncorhynchus mykiss (genetics)</term><term>Open Reading Frames</term><term>Protein Precursors (biosynthesis)</term><term>Protein Precursors (genetics)</term><term>Sequence Homology, Amino Acid</term><term>Vitellogenins (chemistry)</term><term>Vitellogenins (genetics)</term><term>Xenopus laevis (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Protein Precursors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Egg Proteins</term><term>Vitellogenins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Codon</term><term>Egg Proteins</term><term>Protein Precursors</term><term>Vitellogenins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chickens</term><term>Killifishes</term><term>Lampreys</term><term>Oncorhynchus mykiss</term><term>Xenopus laevis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Biological Evolution</term><term>DNA, Complementary</term><term>Female</term><term>Male</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have cloned and sequenced a cDNA encoding a vitellogenin (Vtg) from the mummichog, Fundulus heteroclitus, an estuarine teleost. We constructed a liver cDNA library against RNA from estrogen-treated male mummichogs. Five overlapping cDNA clones totalling 5,197 bp were isolated through a combination of degenerate oligonucleotide probing of the library and PCR. The cDNA sequence contains a 5,112 bp open reading frame. The predicted primary structure of the deduced 1,704-amino-acid protein is 30-40% identical to other documented chordate Vtgs, establishing this Vtg as a member of the ancient Vtg gene family. Of the previously reported chordate Vtg sequences (Xenopus laevis, Gallus domesticus, Ichthyomyzon unicuspis, and Acipenser transmontanus), all four act as precursor proteins to a yolk which is eventually rendered insoluble under physiological conditions, either as crystalline platelets or as noncrystalline granules. The yolk of F. heteroclitus, on the other hand, remains in a soluble state throughout oocyte growth. The putative F. heteroclitus Vtg contains a polyserine region with a relative serine composition that is 10-20% higher than that observed for the other Vtgs. The trinucleotide repeats encoding the characteristic polyserine tracts of the phosvitin region follow a previously reported trend: TCX codons on the 5' end and AGY codons toward the 3' end. Whether the difference in Vtg primary structure between F. heteroclitus and that of other chordates is responsible for the differences in yolk structure remains to be elucidated. As the first complete teleost Vtg to be reported, these data will aid in designing nucleotide and immunological probes for detecting Vtg as a reproductive status indicator in F. heteroclitus and other piscine species.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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