[Molecular characterization and evolutional analysis of liportein lipase and hepatic lipase gene in Chinese Sturgeon and other six freshwater fishes].
Identifieur interne : 000340 ( Ncbi/Curation ); précédent : 000339; suivant : 000341[Molecular characterization and evolutional analysis of liportein lipase and hepatic lipase gene in Chinese Sturgeon and other six freshwater fishes].
Auteurs : Yan Huang [République populaire de Chine] ; Xu-Fang Liang ; Lin Wang ; Guang-Zhao Li ; Xiu-Xia Liu ; Yu YaoSource :
- Dong wu xue yan jiu = Zoological research ; 2010.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Carps, Cricetinae (classification), Cricetinae (genetics), Cricetulus (genetics), DNA, Complementary (analysis), DNA, Complementary (genetics), Evolution, Molecular, Fishes (classification), Fishes (genetics), Fresh Water, Lipase (genetics), Lipoprotein Lipase (analysis), Lipoprotein Lipase (genetics), Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction.
- MESH :
- chemical , analysis : DNA, Complementary, Lipoprotein Lipase.
- classification : Cricetinae, Fishes.
- genetics : Cricetinae, Cricetulus, DNA, Complementary, Fishes, Lipase, Lipoprotein Lipase.
- Amino Acid Sequence, Animals, Base Sequence, Carps, Evolution, Molecular, Fresh Water, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.
DOI: 10.3724/SP.J.1141.2010.03239
PubMed: 20672411
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Molecular characterization and evolutional analysis of liportein lipase and hepatic lipase gene in Chinese Sturgeon and other six freshwater fishes].</title><author><name sortKey="Huang, Yan" sort="Huang, Yan" uniqKey="Huang Y" first="Yan" last="Huang">Yan Huang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science and Technology, Jinan University, Guangzhou 510632, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liang, Xu Fang" sort="Liang, Xu Fang" uniqKey="Liang X" first="Xu-Fang" last="Liang">Xu-Fang Liang</name></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name></author><author><name sortKey="Li, Guang Zhao" sort="Li, Guang Zhao" uniqKey="Li G" first="Guang-Zhao" last="Li">Guang-Zhao Li</name></author><author><name sortKey="Liu, Xiu Xia" sort="Liu, Xiu Xia" uniqKey="Liu X" first="Xiu-Xia" last="Liu">Xiu-Xia Liu</name></author><author><name sortKey="Yao, Yu" sort="Yao, Yu" uniqKey="Yao Y" first="Yu" last="Yao">Yu Yao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20672411</idno><idno type="pmid">20672411</idno><idno type="doi">10.3724/SP.J.1141.2010.03239</idno><idno type="wicri:Area/PubMed/Corpus">000390</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000390</idno><idno type="wicri:Area/PubMed/Curation">000390</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000390</idno><idno type="wicri:Area/PubMed/Checkpoint">000390</idno><idno 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sortKey="Liang, Xu Fang" sort="Liang, Xu Fang" uniqKey="Liang X" first="Xu-Fang" last="Liang">Xu-Fang Liang</name></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name></author><author><name sortKey="Li, Guang Zhao" sort="Li, Guang Zhao" uniqKey="Li G" first="Guang-Zhao" last="Li">Guang-Zhao Li</name></author><author><name sortKey="Liu, Xiu Xia" sort="Liu, Xiu Xia" uniqKey="Liu X" first="Xiu-Xia" last="Liu">Xiu-Xia Liu</name></author><author><name sortKey="Yao, Yu" sort="Yao, Yu" uniqKey="Yao Y" first="Yu" last="Yao">Yu Yao</name></author></analytic><series><title level="j">Dong wu xue yan jiu = Zoological research</title><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Carps</term><term>Cricetinae (classification)</term><term>Cricetinae (genetics)</term><term>Cricetulus (genetics)</term><term>DNA, Complementary (analysis)</term><term>DNA, Complementary (genetics)</term><term>Evolution, Molecular</term><term>Fishes (classification)</term><term>Fishes (genetics)</term><term>Fresh Water</term><term>Lipase (genetics)</term><term>Lipoprotein Lipase (analysis)</term><term>Lipoprotein Lipase (genetics)</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Complementary</term><term>Lipoprotein Lipase</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Cricetinae</term><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cricetinae</term><term>Cricetulus</term><term>DNA, Complementary</term><term>Fishes</term><term>Lipase</term><term>Lipoprotein Lipase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Carps</term><term>Evolution, Molecular</term><term>Fresh Water</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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