Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor.
Identifieur interne : 000309 ( Ncbi/Curation ); précédent : 000308; suivant : 000310Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor.
Auteurs : Zhiyong Liao [République populaire de Chine] ; Xiaoli Chen ; Mingjiang WuSource :
- Science in China. Series C, Life sciences [ 1862-2798 ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, CHO Cells, Cell Proliferation (drug effects), Cloning, Molecular, Cricetinae, Cricetulus, DNA, Complementary (chemistry), DNA, Complementary (genetics), Dose-Response Relationship, Drug, Fish Proteins (genetics), Fish Proteins (metabolism), Fish Proteins (physiology), Fishes (genetics), Gene Expression (drug effects), Gene Expression Profiling, Growth Hormone (metabolism), Growth Hormone (pharmacology), Luciferases (genetics), Luciferases (metabolism), Molecular Sequence Data, Receptors, Somatotropin (genetics), Receptors, Somatotropin (metabolism), Receptors, Somatotropin (physiology), Reverse Transcriptase Polymerase Chain Reaction, Sea Bream (metabolism), Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transfection.
- MESH :
- chemical , chemistry : DNA, Complementary.
- drug effects : Cell Proliferation, Gene Expression.
- chemical , genetics : DNA, Complementary, Fish Proteins, Fishes, Luciferases, Receptors, Somatotropin.
- chemical , metabolism : Fish Proteins, Growth Hormone, Luciferases, Receptors, Somatotropin, Sea Bream.
- chemical , pharmacology : Growth Hormone.
- chemical , physiology : Fish Proteins, Receptors, Somatotropin.
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, CHO Cells, Cloning, Molecular, Cricetinae, Cricetulus, Dose-Response Relationship, Drug, Gene Expression Profiling, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transfection.
Abstract
A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate the mechanism of growth hormone in regulating the growth of Chinese sturgeon. The open reading frame of the cloned Chinese sturgeon growth hormone receptor (csGHR) cDNA encodes a trans-membrane protein of 611 amino acids containing all the characteristic motifs of GHR. By sequence alignment, substitutions of amino acid residues highly conserved in other species were identified. Using the CHO cell culture system, the function of csGHR and the biological significance of the amino acid substitution in csGHR were examined. The promoter of serine protease inhibitor 2.1 (Spi2.1) was trans-activated upon stimulation of seabream GH (sbGH) in the csGHR-expressing CHO cells. Furthermore, CHO cells stably expressing csGHR were stimulated to proliferate by sbGH. In agreement with our previous report, Chinese sturgeon growth hormone-binding protein (csGHBP) was detected in the culture medium of CHO cells stably expressing csGHR. Mutation of Asp residue in the ligand binding motif in csGHR to Glu significantly enhanced csGHR's biological function, whereas mutation of Asp residue to Ala decreased its biological function. The results demonstrated that the cloned csGHR was of full biological function and the csGHBP could be generated through proteolysis of csGHR. These findings might provide new insights into thoroughly understanding the regulatory mechanism of Chinese sturgeon growth.
DOI: 10.1007/s11427-009-0131-3
PubMed: 19911126
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000419
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000419
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000419
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000309
Links to Exploration step
pubmed:19911126Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor.</title>
<author><name sortKey="Liao, Zhiyong" sort="Liao, Zhiyong" uniqKey="Liao Z" first="Zhiyong" last="Liao">Zhiyong Liao</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China. zyliap@sibs.ac.cn</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035</wicri:regionArea>
<wicri:noRegion>325035</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Xiaoli" sort="Chen, Xiaoli" uniqKey="Chen X" first="Xiaoli" last="Chen">Xiaoli Chen</name>
</author>
<author><name sortKey="Wu, Mingjiang" sort="Wu, Mingjiang" uniqKey="Wu M" first="Mingjiang" last="Wu">Mingjiang Wu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19911126</idno>
<idno type="pmid">19911126</idno>
<idno type="doi">10.1007/s11427-009-0131-3</idno>
<idno type="wicri:Area/PubMed/Corpus">000419</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000419</idno>
<idno type="wicri:Area/PubMed/Curation">000419</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000419</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000419</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000419</idno>
<idno type="wicri:Area/Ncbi/Merge">000309</idno>
<idno type="wicri:Area/Ncbi/Curation">000309</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor.</title>
<author><name sortKey="Liao, Zhiyong" sort="Liao, Zhiyong" uniqKey="Liao Z" first="Zhiyong" last="Liao">Zhiyong Liao</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China. zyliap@sibs.ac.cn</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035</wicri:regionArea>
<wicri:noRegion>325035</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Xiaoli" sort="Chen, Xiaoli" uniqKey="Chen X" first="Xiaoli" last="Chen">Xiaoli Chen</name>
</author>
<author><name sortKey="Wu, Mingjiang" sort="Wu, Mingjiang" uniqKey="Wu M" first="Mingjiang" last="Wu">Mingjiang Wu</name>
</author>
</analytic>
<series><title level="j">Science in China. Series C, Life sciences</title>
<idno type="eISSN">1862-2798</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Blotting, Western</term>
<term>CHO Cells</term>
<term>Cell Proliferation (drug effects)</term>
<term>Cloning, Molecular</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>DNA, Complementary (chemistry)</term>
<term>DNA, Complementary (genetics)</term>
<term>Dose-Response Relationship, Drug</term>
<term>Fish Proteins (genetics)</term>
<term>Fish Proteins (metabolism)</term>
<term>Fish Proteins (physiology)</term>
<term>Fishes (genetics)</term>
<term>Gene Expression (drug effects)</term>
<term>Gene Expression Profiling</term>
<term>Growth Hormone (metabolism)</term>
<term>Growth Hormone (pharmacology)</term>
<term>Luciferases (genetics)</term>
<term>Luciferases (metabolism)</term>
<term>Molecular Sequence Data</term>
<term>Receptors, Somatotropin (genetics)</term>
<term>Receptors, Somatotropin (metabolism)</term>
<term>Receptors, Somatotropin (physiology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sea Bream (metabolism)</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Transfection</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term>
<term>Gene Expression</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
<term>Fish Proteins</term>
<term>Fishes</term>
<term>Luciferases</term>
<term>Receptors, Somatotropin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fish Proteins</term>
<term>Growth Hormone</term>
<term>Luciferases</term>
<term>Receptors, Somatotropin</term>
<term>Sea Bream</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Growth Hormone</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Fish Proteins</term>
<term>Receptors, Somatotropin</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Blotting, Western</term>
<term>CHO Cells</term>
<term>Cloning, Molecular</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Dose-Response Relationship, Drug</term>
<term>Gene Expression Profiling</term>
<term>Molecular Sequence Data</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Transfection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate the mechanism of growth hormone in regulating the growth of Chinese sturgeon. The open reading frame of the cloned Chinese sturgeon growth hormone receptor (csGHR) cDNA encodes a trans-membrane protein of 611 amino acids containing all the characteristic motifs of GHR. By sequence alignment, substitutions of amino acid residues highly conserved in other species were identified. Using the CHO cell culture system, the function of csGHR and the biological significance of the amino acid substitution in csGHR were examined. The promoter of serine protease inhibitor 2.1 (Spi2.1) was trans-activated upon stimulation of seabream GH (sbGH) in the csGHR-expressing CHO cells. Furthermore, CHO cells stably expressing csGHR were stimulated to proliferate by sbGH. In agreement with our previous report, Chinese sturgeon growth hormone-binding protein (csGHBP) was detected in the culture medium of CHO cells stably expressing csGHR. Mutation of Asp residue in the ligand binding motif in csGHR to Glu significantly enhanced csGHR's biological function, whereas mutation of Asp residue to Ala decreased its biological function. The results demonstrated that the cloned csGHR was of full biological function and the csGHBP could be generated through proteolysis of csGHR. These findings might provide new insights into thoroughly understanding the regulatory mechanism of Chinese sturgeon growth.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000309 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000309 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:19911126 |texte= Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:19911126" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
![]() | This area was generated with Dilib version V0.6.27. | ![]() |