Characteristics of growth hormone isolated from sturgeon (Acipenser güldenstädti) pituitaries.
Identifieur interne : 000844 ( Ncbi/Checkpoint ); précédent : 000843; suivant : 000845Characteristics of growth hormone isolated from sturgeon (Acipenser güldenstädti) pituitaries.
Auteurs : S W Farmer ; T. Hayashida ; H. Papkoff ; A L PolenovSource :
- Endocrinology [ 0013-7227 ] ; 1981.
English descriptors
- KwdEn :
- Amino Acids (analysis), Animals, Biological Assay, Electrophoresis, Polyacrylamide Gel, Female, Fishes (metabolism), Growth Hormone (analysis), Growth Hormone (isolation & purification), Growth Hormone (metabolism), Male, Molecular Weight, Pituitary Gland (metabolism), Protein Conformation, Species Specificity.
- MESH :
- chemical , analysis : Amino Acids, Growth Hormone.
- chemical , isolation & purification : Growth Hormone.
- metabolism : Fishes, Growth Hormone, Pituitary Gland.
- Animals, Biological Assay, Electrophoresis, Polyacrylamide Gel, Female, Male, Molecular Weight, Protein Conformation, Species Specificity.
Abstract
GH was isolated and characterized from pituitaries of a primitive bony fish, sturgeon (Acipenser güldenstädti). Sturgeon GH was very active in a mammalian GH assay, the rat tibia test. Relative to ovine GH (NIH-GH-S9), sturgeon GH gave a parallel dose-response slope and had a potency of 0.4. Sturgeon GH also showed strong cross-reaction in a snapping turtle GH RIA, comparable to that shown by tetrapod GHs and much greater than that of modern bony fish (teleost) GH. These results, predicted by earlier experiments using pituitary extracts of related species, support the hypothesis that GHs from primitive fish are more closely related to tetrapod GHs than are teleost GHs. Chemical characterization of sturgeon GH, including amino acid terminal residue analyses, amino acid composition, molecular weight determination, and electrophoresis on polyacrylamide gels, indicated that this GH is similar to GHs previously isolated from a teleost and representative species of every tetrapod class. These data provide strong additional evidence that the molecular structure of GH has been highly conserved during evolution. (Endocrinology 108: 377, 1981)
DOI: 10.1210/endo-108-2-377
PubMed: 7449727
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000791
- to stream PubMed, to step Curation: 000791
- to stream PubMed, to step Checkpoint: 000791
- to stream Ncbi, to step Merge: 000844
- to stream Ncbi, to step Curation: 000844
Links to Exploration step
pubmed:7449727Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characteristics of growth hormone isolated from sturgeon (Acipenser güldenstädti) pituitaries.</title>
<author><name sortKey="Farmer, S W" sort="Farmer, S W" uniqKey="Farmer S" first="S W" last="Farmer">S W Farmer</name>
</author>
<author><name sortKey="Hayashida, T" sort="Hayashida, T" uniqKey="Hayashida T" first="T" last="Hayashida">T. Hayashida</name>
</author>
<author><name sortKey="Papkoff, H" sort="Papkoff, H" uniqKey="Papkoff H" first="H" last="Papkoff">H. Papkoff</name>
</author>
<author><name sortKey="Polenov, A L" sort="Polenov, A L" uniqKey="Polenov A" first="A L" last="Polenov">A L Polenov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1981">1981</date>
<idno type="RBID">pubmed:7449727</idno>
<idno type="pmid">7449727</idno>
<idno type="doi">10.1210/endo-108-2-377</idno>
<idno type="wicri:Area/PubMed/Corpus">000791</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000791</idno>
<idno type="wicri:Area/PubMed/Curation">000791</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000791</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000791</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000791</idno>
<idno type="wicri:Area/Ncbi/Merge">000844</idno>
<idno type="wicri:Area/Ncbi/Curation">000844</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000844</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Characteristics of growth hormone isolated from sturgeon (Acipenser güldenstädti) pituitaries.</title>
<author><name sortKey="Farmer, S W" sort="Farmer, S W" uniqKey="Farmer S" first="S W" last="Farmer">S W Farmer</name>
</author>
<author><name sortKey="Hayashida, T" sort="Hayashida, T" uniqKey="Hayashida T" first="T" last="Hayashida">T. Hayashida</name>
</author>
<author><name sortKey="Papkoff, H" sort="Papkoff, H" uniqKey="Papkoff H" first="H" last="Papkoff">H. Papkoff</name>
</author>
<author><name sortKey="Polenov, A L" sort="Polenov, A L" uniqKey="Polenov A" first="A L" last="Polenov">A L Polenov</name>
</author>
</analytic>
<series><title level="j">Endocrinology</title>
<idno type="ISSN">0013-7227</idno>
<imprint><date when="1981" type="published">1981</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (analysis)</term>
<term>Animals</term>
<term>Biological Assay</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Female</term>
<term>Fishes (metabolism)</term>
<term>Growth Hormone (analysis)</term>
<term>Growth Hormone (isolation & purification)</term>
<term>Growth Hormone (metabolism)</term>
<term>Male</term>
<term>Molecular Weight</term>
<term>Pituitary Gland (metabolism)</term>
<term>Protein Conformation</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Amino Acids</term>
<term>Growth Hormone</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Growth Hormone</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term>
<term>Growth Hormone</term>
<term>Pituitary Gland</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Biological Assay</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Female</term>
<term>Male</term>
<term>Molecular Weight</term>
<term>Protein Conformation</term>
<term>Species Specificity</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">GH was isolated and characterized from pituitaries of a primitive bony fish, sturgeon (Acipenser güldenstädti). Sturgeon GH was very active in a mammalian GH assay, the rat tibia test. Relative to ovine GH (NIH-GH-S9), sturgeon GH gave a parallel dose-response slope and had a potency of 0.4. Sturgeon GH also showed strong cross-reaction in a snapping turtle GH RIA, comparable to that shown by tetrapod GHs and much greater than that of modern bony fish (teleost) GH. These results, predicted by earlier experiments using pituitary extracts of related species, support the hypothesis that GHs from primitive fish are more closely related to tetrapod GHs than are teleost GHs. Chemical characterization of sturgeon GH, including amino acid terminal residue analyses, amino acid composition, molecular weight determination, and electrophoresis on polyacrylamide gels, indicated that this GH is similar to GHs previously isolated from a teleost and representative species of every tetrapod class. These data provide strong additional evidence that the molecular structure of GH has been highly conserved during evolution. (Endocrinology 108: 377, 1981)</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Farmer, S W" sort="Farmer, S W" uniqKey="Farmer S" first="S W" last="Farmer">S W Farmer</name>
<name sortKey="Hayashida, T" sort="Hayashida, T" uniqKey="Hayashida T" first="T" last="Hayashida">T. Hayashida</name>
<name sortKey="Papkoff, H" sort="Papkoff, H" uniqKey="Papkoff H" first="H" last="Papkoff">H. Papkoff</name>
<name sortKey="Polenov, A L" sort="Polenov, A L" uniqKey="Polenov A" first="A L" last="Polenov">A L Polenov</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000844 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000844 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:7449727 |texte= Characteristics of growth hormone isolated from sturgeon (Acipenser güldenstädti) pituitaries. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:7449727" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |