Sturgeon nucleo-cytoplasmic large DNA virus phylogeny and PCR tests.
Identifieur interne : 000670 ( Ncbi/Checkpoint ); précédent : 000669; suivant : 000671Sturgeon nucleo-cytoplasmic large DNA virus phylogeny and PCR tests.
Auteurs : Sharon C. Clouthier [Canada] ; Elissa Vanwalleghem ; Eric D. AndersonSource :
- Diseases of aquatic organisms [ 0177-5103 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Viral.
- geographic : Manitoba.
- genetics : DNA Viruses.
- methods : Polymerase Chain Reaction.
- physiology : Gene Expression Regulation, Viral.
- veterinary : DNA Virus Infections.
- virology : Fish Diseases.
- Animals, Fishes, Phylogeny, Seasons.
Abstract
Sturgeon epitheliotropic nucleo-cytoplasmic large DNA viruses (NCLDVs) can cause a lethal disease of the integumentary system. These viruses have not been assigned to any currently recognized family or genus. In this study, phylogenetic analyses using the major capsid protein (MCP) showed that the sturgeon NCLDVs formed a cohesive taxonomic group, could be identified to the species or possibly sub-species level and formed a distinct evolutionary lineage within the Megavirales. The genetic relatedness of the sturgeon virus MCP allowed design of 3 PCR diagnostic tests with analytical specificity (ASp) inclusive of this group of viruses. The conventional PCR test, C1, had broader ASp than the 2 quantitative PCR tests, Q1 and Q2, and was inclusive of the sturgeon viruses as well as some viruses belonging to the families Mimi-, Phycodna-, or Iridoviridae. Q2 had broader specificity than Q1 but both tests recognized the sturgeon NCLDVs and did not cross-react with co-localizing sturgeon herpesviruses. Analytical test performance characteristics evaluated for Q1 and Q2 revealed sensitive assays with observed 50% limits of detection between 3 and 6.25 plasmid copies and high intra- and inter-assay repeatability. Q1 was used to test for sturgeon viruses in endangered populations of lake sturgeon Acipenser fulvescens within the Winnipeg River or Nelson River drainage systems of Manitoba, Canada. Test results indicated that namao virus is endemic in the Nelson River water basin. These tests meet the analytical requirements for diagnostic testing in Canada and are useful tools for disease management in sturgeon conservation stocking programs in North America.
DOI: 10.3354/dao02937
PubMed: 26648102
Affiliations:
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pubmed:26648102Le document en format XML
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Clouthier</name><affiliation wicri:level="1"><nlm:affiliation>Fisheries & Oceans Canada, Freshwater Institute, 501 University Crescent, Winnipeg, Manitoba R3T 2N6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Fisheries & Oceans Canada, Freshwater Institute, 501 University Crescent, Winnipeg, Manitoba R3T 2N6</wicri:regionArea><wicri:noRegion>Manitoba R3T 2N6</wicri:noRegion></affiliation></author><author><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></author><author><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. 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Clouthier</name><affiliation wicri:level="1"><nlm:affiliation>Fisheries & Oceans Canada, Freshwater Institute, 501 University Crescent, Winnipeg, Manitoba R3T 2N6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Fisheries & Oceans Canada, Freshwater Institute, 501 University Crescent, Winnipeg, Manitoba R3T 2N6</wicri:regionArea><wicri:noRegion>Manitoba R3T 2N6</wicri:noRegion></affiliation></author><author><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></author><author><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. Anderson</name></author></analytic><series><title level="j">Diseases of aquatic organisms</title><idno type="ISSN">0177-5103</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>DNA Virus Infections (veterinary)</term><term>DNA Viruses (genetics)</term><term>DNA, Viral (genetics)</term><term>Fish Diseases (virology)</term><term>Fishes</term><term>Gene Expression Regulation, Viral (physiology)</term><term>Manitoba</term><term>Phylogeny</term><term>Polymerase Chain Reaction (methods)</term><term>Seasons</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Manitoba</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>DNA Viruses</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Viral</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>DNA Virus Infections</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Fish Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fishes</term><term>Phylogeny</term><term>Seasons</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sturgeon epitheliotropic nucleo-cytoplasmic large DNA viruses (NCLDVs) can cause a lethal disease of the integumentary system. These viruses have not been assigned to any currently recognized family or genus. In this study, phylogenetic analyses using the major capsid protein (MCP) showed that the sturgeon NCLDVs formed a cohesive taxonomic group, could be identified to the species or possibly sub-species level and formed a distinct evolutionary lineage within the Megavirales. The genetic relatedness of the sturgeon virus MCP allowed design of 3 PCR diagnostic tests with analytical specificity (ASp) inclusive of this group of viruses. The conventional PCR test, C1, had broader ASp than the 2 quantitative PCR tests, Q1 and Q2, and was inclusive of the sturgeon viruses as well as some viruses belonging to the families Mimi-, Phycodna-, or Iridoviridae. Q2 had broader specificity than Q1 but both tests recognized the sturgeon NCLDVs and did not cross-react with co-localizing sturgeon herpesviruses. Analytical test performance characteristics evaluated for Q1 and Q2 revealed sensitive assays with observed 50% limits of detection between 3 and 6.25 plasmid copies and high intra- and inter-assay repeatability. Q1 was used to test for sturgeon viruses in endangered populations of lake sturgeon Acipenser fulvescens within the Winnipeg River or Nelson River drainage systems of Manitoba, Canada. Test results indicated that namao virus is endemic in the Nelson River water basin. These tests meet the analytical requirements for diagnostic testing in Canada and are useful tools for disease management in sturgeon conservation stocking programs in North America.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. Anderson</name><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></noCountry><country name="Canada"><noRegion><name sortKey="Clouthier, Sharon C" sort="Clouthier, Sharon C" uniqKey="Clouthier S" first="Sharon C" last="Clouthier">Sharon C. Clouthier</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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