Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology.
Identifieur interne : 000645 ( Ncbi/Checkpoint ); précédent : 000644; suivant : 000646Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology.
Auteurs : L M Li [République populaire de Chine] ; L. Wei [République populaire de Chine] ; H Y Jiang [République populaire de Chine] ; Y. Zhang [République populaire de Chine] ; X J Zhang [République populaire de Chine] ; L H Yuan [République populaire de Chine] ; J P Chen [République populaire de Chine]Source :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- genetics : Fishes, Microsatellite Repeats.
- methods : High-Throughput Nucleotide Sequencing.
- Animals, Molecular Sequence Data, Polymorphism, Genetic.
Abstract
Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of ob-served alleles per locus is 6.6 (ranging from 2 to 17). Observed and expected heterozygosity values ranged from 0 to 0.958 and from 0.508 to 0.940, respectively. Not a single locus showed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was observed among any pairwise loci. These highly informative microsatellite markers will be useful for genetic diversity and population structure analyses of A. schrenckii and other species of this genus.
DOI: 10.4238/2015.July.14.16
PubMed: 26214471
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000124
- to stream PubMed, to step Curation: 000124
- to stream PubMed, to step Checkpoint: 000124
- to stream Ncbi, to step Merge: 000645
- to stream Ncbi, to step Curation: 000645
Links to Exploration step
pubmed:26214471Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology.</title>
<author><name sortKey="Li, L M" sort="Li, L M" uniqKey="Li L" first="L M" last="Li">L M Li</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wei, L" sort="Wei, L" uniqKey="Wei L" first="L" last="Wei">L. Wei</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Ecology, Lishui University, Lishui, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Ecology, Lishui University, Lishui</wicri:regionArea>
<wicri:noRegion>Lishui</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jiang, H Y" sort="Jiang, H Y" uniqKey="Jiang H" first="H Y" last="Jiang">H Y Jiang</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Y" sort="Zhang, Y" uniqKey="Zhang Y" first="Y" last="Zhang">Y. Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science of Inner Mongolia Agriculture University, Hohhot, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science of Inner Mongolia Agriculture University, Hohhot</wicri:regionArea>
<wicri:noRegion>Hohhot</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, X J" sort="Zhang, X J" uniqKey="Zhang X" first="X J" last="Zhang">X J Zhang</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yuan, L H" sort="Yuan, L H" uniqKey="Yuan L" first="L H" last="Yuan">L H Yuan</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, J P" sort="Chen, J P" uniqKey="Chen J" first="J P" last="Chen">J P Chen</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China chenjp@gdei.gd.cn.</nlm:affiliation>
<country wicri:rule="url">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2015">2015</date>
<idno type="RBID">pubmed:26214471</idno>
<idno type="pmid">26214471</idno>
<idno type="doi">10.4238/2015.July.14.16</idno>
<idno type="wicri:Area/PubMed/Corpus">000124</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000124</idno>
<idno type="wicri:Area/PubMed/Curation">000124</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000124</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000124</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000124</idno>
<idno type="wicri:Area/Ncbi/Merge">000645</idno>
<idno type="wicri:Area/Ncbi/Curation">000645</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000645</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology.</title>
<author><name sortKey="Li, L M" sort="Li, L M" uniqKey="Li L" first="L M" last="Li">L M Li</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wei, L" sort="Wei, L" uniqKey="Wei L" first="L" last="Wei">L. Wei</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Ecology, Lishui University, Lishui, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Ecology, Lishui University, Lishui</wicri:regionArea>
<wicri:noRegion>Lishui</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jiang, H Y" sort="Jiang, H Y" uniqKey="Jiang H" first="H Y" last="Jiang">H Y Jiang</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Y" sort="Zhang, Y" uniqKey="Zhang Y" first="Y" last="Zhang">Y. Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science of Inner Mongolia Agriculture University, Hohhot, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science of Inner Mongolia Agriculture University, Hohhot</wicri:regionArea>
<wicri:noRegion>Hohhot</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, X J" sort="Zhang, X J" uniqKey="Zhang X" first="X J" last="Zhang">X J Zhang</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yuan, L H" sort="Yuan, L H" uniqKey="Yuan L" first="L H" last="Yuan">L H Yuan</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, J P" sort="Chen, J P" uniqKey="Chen J" first="J P" last="Chen">J P Chen</name>
<affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute, Guangzhou, China chenjp@gdei.gd.cn.</nlm:affiliation>
<country wicri:rule="url">République populaire de Chine</country>
<wicri:regionArea>Guangdong Entomological Institute, Guangzhou</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Genetics and molecular research : GMR</title>
<idno type="eISSN">1676-5680</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Fishes (genetics)</term>
<term>High-Throughput Nucleotide Sequencing (methods)</term>
<term>Microsatellite Repeats (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Polymorphism, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term>
<term>Microsatellite Repeats</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>High-Throughput Nucleotide Sequencing</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Molecular Sequence Data</term>
<term>Polymorphism, Genetic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of ob-served alleles per locus is 6.6 (ranging from 2 to 17). Observed and expected heterozygosity values ranged from 0 to 0.958 and from 0.508 to 0.940, respectively. Not a single locus showed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was observed among any pairwise loci. These highly informative microsatellite markers will be useful for genetic diversity and population structure analyses of A. schrenckii and other species of this genus.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Guangdong</li>
</region>
<settlement><li>Jiangmen</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Li, L M" sort="Li, L M" uniqKey="Li L" first="L M" last="Li">L M Li</name>
</region>
<name sortKey="Chen, J P" sort="Chen, J P" uniqKey="Chen J" first="J P" last="Chen">J P Chen</name>
<name sortKey="Jiang, H Y" sort="Jiang, H Y" uniqKey="Jiang H" first="H Y" last="Jiang">H Y Jiang</name>
<name sortKey="Wei, L" sort="Wei, L" uniqKey="Wei L" first="L" last="Wei">L. Wei</name>
<name sortKey="Yuan, L H" sort="Yuan, L H" uniqKey="Yuan L" first="L H" last="Yuan">L H Yuan</name>
<name sortKey="Zhang, X J" sort="Zhang, X J" uniqKey="Zhang X" first="X J" last="Zhang">X J Zhang</name>
<name sortKey="Zhang, Y" sort="Zhang, Y" uniqKey="Zhang Y" first="Y" last="Zhang">Y. Zhang</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000645 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000645 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:26214471 |texte= Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:26214471" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |