Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO.
Identifieur interne : 000249 ( Ncbi/Checkpoint ); précédent : 000248; suivant : 000250Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO.
Auteurs : Martin Psenicka [République tchèque] ; Grzegorz J. Dietrich ; Mariola Wojtczak ; Joanna Nynca ; Marek Rodina ; Otomar Linhart ; Jacky Cosson ; Andrzej CiereszkoSource :
- Cryobiology [ 1090-2392 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- chemical , toxicity : Cryoprotective Agents, Dimethyl Sulfoxide, Methanol.
- cytology : Spermatozoa.
- drug effects : Sperm Motility.
- physiology : Acrosome, Spermatozoa.
- Animals, Cryopreservation, Fishes, Fluorescent Dyes, Male, Staining and Labeling.
Abstract
In this study we describe acrosome staining and motility characteristics of fresh and cryopreserved sterlet (Acipenser ruthenus L.) spermatozoa using soybean trypsin inhibitor-Alexa conjugate fluorescent staining and computer-aided sperm analysis (CASA), respectively. Methanol or dimethylsulfoxide (DMSO) were used as cryoprotectants. After cryopreservation a decline in sperm motility characteristics occurred, but no differential effect between cryoprotectant was observed. Cryopreservation caused a significant increase in the percentage of spermatozoa with acrosome stained by SBTI-Alexa for samples cryopreserved using DMSO compared to methanol. These data suggest that the low usefulness of DMSO for cryopreservation of sturgeon spermatozoa is related to its harmful specific effect towards the acrosome, probably by causing its precocious triggering, much before any egg contact.
DOI: 10.1016/j.cryobiol.2008.03.006
PubMed: 18466892
Affiliations:
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Dietrich</name></author><author><name sortKey="Wojtczak, Mariola" sort="Wojtczak, Mariola" uniqKey="Wojtczak M" first="Mariola" last="Wojtczak">Mariola Wojtczak</name></author><author><name sortKey="Nynca, Joanna" sort="Nynca, Joanna" uniqKey="Nynca J" first="Joanna" last="Nynca">Joanna Nynca</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name></author><author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name></author><author><name sortKey="Ciereszko, Andrzej" sort="Ciereszko, Andrzej" uniqKey="Ciereszko A" first="Andrzej" last="Ciereszko">Andrzej Ciereszko</name></author></analytic><series><title level="j">Cryobiology</title><idno type="eISSN">1090-2392</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrosome (physiology)</term><term>Animals</term><term>Cryopreservation</term><term>Cryoprotective Agents (toxicity)</term><term>Dimethyl Sulfoxide (toxicity)</term><term>Fishes</term><term>Fluorescent Dyes</term><term>Male</term><term>Methanol (toxicity)</term><term>Sperm Motility (drug effects)</term><term>Spermatozoa (cytology)</term><term>Spermatozoa (physiology)</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cryoprotective Agents</term><term>Dimethyl Sulfoxide</term><term>Methanol</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Sperm Motility</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Acrosome</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cryopreservation</term><term>Fishes</term><term>Fluorescent Dyes</term><term>Male</term><term>Staining and Labeling</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study we describe acrosome staining and motility characteristics of fresh and cryopreserved sterlet (Acipenser ruthenus L.) spermatozoa using soybean trypsin inhibitor-Alexa conjugate fluorescent staining and computer-aided sperm analysis (CASA), respectively. Methanol or dimethylsulfoxide (DMSO) were used as cryoprotectants. After cryopreservation a decline in sperm motility characteristics occurred, but no differential effect between cryoprotectant was observed. Cryopreservation caused a significant increase in the percentage of spermatozoa with acrosome stained by SBTI-Alexa for samples cryopreserved using DMSO compared to methanol. These data suggest that the low usefulness of DMSO for cryopreservation of sturgeon spermatozoa is related to its harmful specific effect towards the acrosome, probably by causing its precocious triggering, much before any egg contact.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Ciereszko, Andrzej" sort="Ciereszko, Andrzej" uniqKey="Ciereszko A" first="Andrzej" last="Ciereszko">Andrzej Ciereszko</name><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name><name sortKey="Dietrich, Grzegorz J" sort="Dietrich, Grzegorz J" uniqKey="Dietrich G" first="Grzegorz J" last="Dietrich">Grzegorz J. Dietrich</name><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><name sortKey="Nynca, Joanna" sort="Nynca, Joanna" uniqKey="Nynca J" first="Joanna" last="Nynca">Joanna Nynca</name><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><name sortKey="Wojtczak, Mariola" sort="Wojtczak, Mariola" uniqKey="Wojtczak M" first="Mariola" last="Wojtczak">Mariola Wojtczak</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Psenicka, Martin" sort="Psenicka, Martin" uniqKey="Psenicka M" first="Martin" last="Psenicka">Martin Psenicka</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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