Salinity effects on activity and expression of glutathione S-transferases in white sturgeon and Chinook salmon.
Identifieur interne : 000166 ( Ncbi/Checkpoint ); précédent : 000165; suivant : 000167Salinity effects on activity and expression of glutathione S-transferases in white sturgeon and Chinook salmon.
Auteurs : Rachel T. Donham [États-Unis] ; Dexter Morin ; Ronald S. TjeerdemaSource :
- Ecotoxicology and environmental safety [ 0147-6513 ] ; 2006.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glutathione, Glutathione Transferase.
- chemical , pharmacology : Sodium Chloride.
- chemical , toxicity : Dinitrochlorobenzene, Ethacrynic Acid.
- enzymology : Liver.
- metabolism : Fishes, Salmon.
- Animals.
Abstract
This study evaluated the activity and expression of the glutathione S-transferase (GST) detoxification isoenzymes in juvenile white sturgeon (Acipenser transmontanus) and Chinook salmon (Oncorhynchus tshawytscha) during acclimation from freshwater (2 per thousand) to estuarine (15 per thousand) salinity conditions. In white sturgeon, GST activity toward 1-chloro-2,4-dinitrobenzene (CDNB) increased significantly (P = 0.005; n = 5) with elevated salinity, but not for the Chinook salmon (P = 0.174; n = 10). GST activity of both sturgeon and salmon toward ethacrynic acid (ETHA) did not significantly change with elevated salinity (P = 0.516 with n = 3, and P = 0.125 with n = 3, respectively). Expression of the GST classes, and hepatic glutathione (GSH) concentration, as determined by HPLC, also did not significantly change with increased salinity. In conclusion, overall GST activity in white sturgeon, but not Chinook salmon, is stimulated by elevated water salinity, thus electrophilic chemicals such as pesticides may be more effectively detoxified by sturgeon as they undergo seaward migration.
DOI: 10.1016/j.ecoenv.2005.01.007
PubMed: 16677913
Affiliations:
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Donham</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Morin, Dexter" sort="Morin, Dexter" uniqKey="Morin D" first="Dexter" last="Morin">Dexter Morin</name></author><author><name sortKey="Tjeerdema, Ronald S" sort="Tjeerdema, Ronald S" uniqKey="Tjeerdema R" first="Ronald S" last="Tjeerdema">Ronald S. 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Donham</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Morin, Dexter" sort="Morin, Dexter" uniqKey="Morin D" first="Dexter" last="Morin">Dexter Morin</name></author><author><name sortKey="Tjeerdema, Ronald S" sort="Tjeerdema, Ronald S" uniqKey="Tjeerdema R" first="Ronald S" last="Tjeerdema">Ronald S. Tjeerdema</name></author></analytic><series><title level="j">Ecotoxicology and environmental safety</title><idno type="ISSN">0147-6513</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Dinitrochlorobenzene (toxicity)</term><term>Ethacrynic Acid (toxicity)</term><term>Fishes (metabolism)</term><term>Glutathione (metabolism)</term><term>Glutathione Transferase (metabolism)</term><term>Liver (enzymology)</term><term>Salmon (metabolism)</term><term>Sodium Chloride (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione</term><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sodium Chloride</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Dinitrochlorobenzene</term><term>Ethacrynic Acid</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Salmon</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study evaluated the activity and expression of the glutathione S-transferase (GST) detoxification isoenzymes in juvenile white sturgeon (Acipenser transmontanus) and Chinook salmon (Oncorhynchus tshawytscha) during acclimation from freshwater (2 per thousand) to estuarine (15 per thousand) salinity conditions. In white sturgeon, GST activity toward 1-chloro-2,4-dinitrobenzene (CDNB) increased significantly (P = 0.005; n = 5) with elevated salinity, but not for the Chinook salmon (P = 0.174; n = 10). GST activity of both sturgeon and salmon toward ethacrynic acid (ETHA) did not significantly change with elevated salinity (P = 0.516 with n = 3, and P = 0.125 with n = 3, respectively). Expression of the GST classes, and hepatic glutathione (GSH) concentration, as determined by HPLC, also did not significantly change with increased salinity. In conclusion, overall GST activity in white sturgeon, but not Chinook salmon, is stimulated by elevated water salinity, thus electrophilic chemicals such as pesticides may be more effectively detoxified by sturgeon as they undergo seaward migration.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Morin, Dexter" sort="Morin, Dexter" uniqKey="Morin D" first="Dexter" last="Morin">Dexter Morin</name><name sortKey="Tjeerdema, Ronald S" sort="Tjeerdema, Ronald S" uniqKey="Tjeerdema R" first="Ronald S" last="Tjeerdema">Ronald S. Tjeerdema</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Donham, Rachel T" sort="Donham, Rachel T" uniqKey="Donham R" first="Rachel T" last="Donham">Rachel T. Donham</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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