Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA
Identifieur interne : 001E72 ( Main/Merge ); précédent : 001E71; suivant : 001E73Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA
Auteurs : C. A. Bidwell [États-Unis] ; K. J. Kroll ; E. Severud ; S. I. Doroshov ; D. M. CarlsonSource :
- General and comparative endocrinology [ 0016-6480 ] ; 1991.
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Abstract
Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogen-treated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon.
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Pascal:92-0677371Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA</title><author><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C. A." last="Bidwell">C. A. Bidwell</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. California, dep. animal sci.</s1><s2>Davis CA 95616</s2><s3>USA</s3></inist:fA14><country>États-Unis</country><wicri:noRegion>Davis CA 95616</wicri:noRegion></affiliation></author><author><name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K. J." last="Kroll">K. J. Kroll</name></author><author><name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E." last="Severud">E. Severud</name></author><author><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S. I." last="Doroshov">S. I. Doroshov</name></author><author><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D. M." last="Carlson">D. M. Carlson</name></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">92-0677371</idno><date when="1991">1991</date><idno type="stanalyst">PASCAL 92-0677371 INIST</idno><idno type="RBID">Pascal:92-0677371</idno><idno type="wicri:Area/PascalFrancis/Corpus">000571</idno><idno type="wicri:Area/PascalFrancis/Curation">000541</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000585</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000585</idno><idno type="wicri:doubleKey">0016-6480:1991:Bidwell C:identification:and:preliminary</idno><idno type="wicri:Area/Main/Merge">001E72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA</title><author><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C. A." last="Bidwell">C. A. Bidwell</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. California, dep. animal sci.</s1><s2>Davis CA 95616</s2><s3>USA</s3></inist:fA14><country>États-Unis</country><wicri:noRegion>Davis CA 95616</wicri:noRegion></affiliation></author><author><name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K. J." last="Kroll">K. J. Kroll</name></author><author><name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E." last="Severud">E. Severud</name></author><author><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S. I." last="Doroshov">S. I. Doroshov</name></author><author><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D. M." last="Carlson">D. M. Carlson</name></author></analytic><series><title level="j" type="main">General and comparative endocrinology</title><title level="j" type="abbreviated">Gen. comp. endocrinol.</title><idno type="ISSN">0016-6480</idno><imprint><date when="1991">1991</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">General and comparative endocrinology</title><title level="j" type="abbreviated">Gen. comp. endocrinol.</title><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term><term>Characterization</term><term>Estrogen</term><term>Gel electrophoresis</term><term>Immunoprecipitation reaction</term><term>Messenger RNA</term><term>Phosphoproteins</term><term>Pisces</term><term>Vitellogenesis</term><term>Vitellogenin</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Pisces</term><term>Vitellogénine</term><term>RNA messager</term><term>Caractérisation</term><term>Electrophorèse gel</term><term>Immunoprécipitation</term><term>Phosphoprotéine</term><term>Oestrogène</term><term>Vitellogenèse</term><term>Acipenser transmontanus</term><term>Acipenseridae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogen-treated sturgeon. Cell-free translation of poly(A)<sup>+</sup> mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D. M." last="Carlson">D. M. Carlson</name><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S. I." last="Doroshov">S. I. Doroshov</name><name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K. J." last="Kroll">K. J. Kroll</name><name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E." last="Severud">E. Severud</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C. A." last="Bidwell">C. A. Bidwell</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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