Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA
Identifieur interne : 001E72 ( Main/Merge ); précédent : 001E71; suivant : 001E73Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA
Auteurs : C. A. Bidwell [États-Unis] ; K. J. Kroll ; E. Severud ; S. I. Doroshov ; D. M. CarlsonSource :
- General and comparative endocrinology [ 0016-6480 ] ; 1991.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogen-treated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon.
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Pascal:92-0677371Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA</title>
<author><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C. A." last="Bidwell">C. A. Bidwell</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. California, dep. animal sci.</s1>
<s2>Davis CA 95616</s2>
<s3>USA</s3>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis CA 95616</wicri:noRegion>
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</author>
<author><name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K. J." last="Kroll">K. J. Kroll</name>
</author>
<author><name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E." last="Severud">E. Severud</name>
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<author><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S. I." last="Doroshov">S. I. Doroshov</name>
</author>
<author><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D. M." last="Carlson">D. M. Carlson</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA</title>
<author><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C. A." last="Bidwell">C. A. Bidwell</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. California, dep. animal sci.</s1>
<s2>Davis CA 95616</s2>
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<author><name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K. J." last="Kroll">K. J. Kroll</name>
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<author><name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E." last="Severud">E. Severud</name>
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<author><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S. I." last="Doroshov">S. I. Doroshov</name>
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<author><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D. M." last="Carlson">D. M. Carlson</name>
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<series><title level="j" type="main">General and comparative endocrinology</title>
<title level="j" type="abbreviated">Gen. comp. endocrinol.</title>
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<seriesStmt><title level="j" type="main">General and comparative endocrinology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term>
<term>Characterization</term>
<term>Estrogen</term>
<term>Gel electrophoresis</term>
<term>Immunoprecipitation reaction</term>
<term>Messenger RNA</term>
<term>Phosphoproteins</term>
<term>Pisces</term>
<term>Vitellogenesis</term>
<term>Vitellogenin</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Pisces</term>
<term>Vitellogénine</term>
<term>RNA messager</term>
<term>Caractérisation</term>
<term>Electrophorèse gel</term>
<term>Immunoprécipitation</term>
<term>Phosphoprotéine</term>
<term>Oestrogène</term>
<term>Vitellogenèse</term>
<term>Acipenser transmontanus</term>
<term>Acipenseridae</term>
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<front><div type="abstract" xml:lang="en">Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogen-treated sturgeon. Cell-free translation of poly(A)<sup>+</sup>
mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon.</div>
</front>
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<affiliations><list><country><li>États-Unis</li>
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<tree><noCountry><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D. M." last="Carlson">D. M. Carlson</name>
<name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S. I." last="Doroshov">S. I. Doroshov</name>
<name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K. J." last="Kroll">K. J. Kroll</name>
<name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E." last="Severud">E. Severud</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C. A." last="Bidwell">C. A. Bidwell</name>
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