Identification and preliminary characterization of white sturgeon ( Acipenser transmontanus ) vitellogenin mRNA
Identifieur interne : 001E36 ( Main/Merge ); précédent : 001E35; suivant : 001E37Identification and preliminary characterization of white sturgeon ( Acipenser transmontanus ) vitellogenin mRNA
Auteurs : Christopher A. Bidwell [États-Unis] ; Kevin J. Kroll [États-Unis] ; Erik Severud [États-Unis] ; Serge I. Doroshov [États-Unis] ; Don M. Carlson [États-Unis]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1991.
Abstract
Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogentreated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon. Immunoprecipitations also showed the presence of four other serum proteins synthesized by the liver of estrogen-treated sturgeon. The induction of vitellogenesis by estrogen in sturgeon, which are a primitive teleost, was found to be similar to induction of vitellogenesis in amphibians, avians, and other teleosts. Estrogen treatment induced a highly abundant vitellogenin mRNA as well as several mRNAs for other serum proteins. However, the presence of two distinct vitellogenin monomers in the cell-free translation assay was significantly different from the results in other species.
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DOI: 10.1016/0016-6480(91)90147-X
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Bidwell</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Animal Science, University of California, Davis, Davis</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>1 Present address: Department of Animal Sciences, Purdue University, West Lafayette</wicri:cityArea></affiliation></author><author><name sortKey="Kroll, Kevin J" sort="Kroll, Kevin J" uniqKey="Kroll K" first="Kevin J." last="Kroll">Kevin J. 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Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogentreated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon. Immunoprecipitations also showed the presence of four other serum proteins synthesized by the liver of estrogen-treated sturgeon. The induction of vitellogenesis by estrogen in sturgeon, which are a primitive teleost, was found to be similar to induction of vitellogenesis in amphibians, avians, and other teleosts. Estrogen treatment induced a highly abundant vitellogenin mRNA as well as several mRNAs for other serum proteins. However, the presence of two distinct vitellogenin monomers in the cell-free translation assay was significantly different from the results in other species.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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