Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA.
Identifieur interne : 001E16 ( Main/Merge ); précédent : 001E15; suivant : 001E17Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA.
Auteurs : C A Bidwell ; K J Kroll ; E. Severud ; S I Doroshov ; D M CarlsonSource :
- General and comparative endocrinology [ 0016-6480 ] ; 1991.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : RNA, Messenger.
- chemical , genetics : Vitellogenins.
- chemical , pharmacology : Estradiol.
- drug effects : Liver.
- genetics : Fishes.
- metabolism : Liver.
- physiology : Reproduction.
- Animals, Cell-Free System, Cloning, Molecular.
Abstract
Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogen-treated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon. Immunoprecipitations also showed the presence of four other serum proteins synthesized by the liver of estrogen-treated sturgeon. The induction of vitellogenesis by estrogen in sturgeon, which are a primitive teleost, was found to be similar to induction of vitellogenesis in amphibians, avians, and other teleosts. Estrogen treatment induced a highly abundant vitellogenin mRNA as well as several mRNAs for other serum proteins. However, the presence of two distinct vitellogenin monomers in the cell-free translation assay was significantly different from the results in other species.
PubMed: 1936922
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pubmed:1936922Le document en format XML
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Severud</name></author><author><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S I" last="Doroshov">S I Doroshov</name></author><author><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D M" last="Carlson">D M Carlson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:1936922</idno><idno type="pmid">1936922</idno><idno type="wicri:Area/PubMed/Corpus">000734</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000734</idno><idno type="wicri:Area/PubMed/Curation">000734</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000734</idno><idno type="wicri:Area/PubMed/Checkpoint">000734</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000734</idno><idno type="wicri:Area/Ncbi/Merge">000283</idno><idno type="wicri:Area/Ncbi/Curation">000283</idno><idno type="wicri:Area/Ncbi/Checkpoint">000283</idno><idno type="wicri:doubleKey">0016-6480:1991:Bidwell C:identification:and:preliminary</idno><idno type="wicri:Area/Main/Merge">001E16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA.</title><author><name sortKey="Bidwell, C A" sort="Bidwell, C A" uniqKey="Bidwell C" first="C A" last="Bidwell">C A Bidwell</name><affiliation><nlm:affiliation>Department of Animal Science, University of California, Davis 95616.</nlm:affiliation><wicri:noCountry code="subField">Davis 95616</wicri:noCountry></affiliation></author><author><name sortKey="Kroll, K J" sort="Kroll, K J" uniqKey="Kroll K" first="K J" last="Kroll">K J Kroll</name></author><author><name sortKey="Severud, E" sort="Severud, E" uniqKey="Severud E" first="E" last="Severud">E. Severud</name></author><author><name sortKey="Doroshov, S I" sort="Doroshov, S I" uniqKey="Doroshov S" first="S I" last="Doroshov">S I Doroshov</name></author><author><name sortKey="Carlson, D M" sort="Carlson, D M" uniqKey="Carlson D" first="D M" last="Carlson">D M Carlson</name></author></analytic><series><title level="j">General and comparative endocrinology</title><idno type="ISSN">0016-6480</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell-Free System</term><term>Cloning, Molecular</term><term>Estradiol (pharmacology)</term><term>Fishes (genetics)</term><term>Liver (drug effects)</term><term>Liver (metabolism)</term><term>RNA, Messenger (biosynthesis)</term><term>Reproduction (physiology)</term><term>Vitellogenins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Vitellogenins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Estradiol</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Reproduction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell-Free System</term><term>Cloning, Molecular</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogen-treated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon. Immunoprecipitations also showed the presence of four other serum proteins synthesized by the liver of estrogen-treated sturgeon. The induction of vitellogenesis by estrogen in sturgeon, which are a primitive teleost, was found to be similar to induction of vitellogenesis in amphibians, avians, and other teleosts. Estrogen treatment induced a highly abundant vitellogenin mRNA as well as several mRNAs for other serum proteins. However, the presence of two distinct vitellogenin monomers in the cell-free translation assay was significantly different from the results in other species.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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