Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals
Identifieur interne : 001C80 ( Main/Merge ); précédent : 001C79; suivant : 001C81Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals
Auteurs : C. Pelissero [Royaume-Uni] ; G. Flouriot [France] ; J. L. Foucher [France] ; B. Bennetau [France] ; J. Dunoguès [France] ; F. Le Gac [France] ; J. P. Sumpter [Royaume-Uni]Source :
- Journal of Steroid Biochemistry and Molecular Biology [ 0960-0760 ] ; 1993.
Abstract
We describe here an in vitro technique to assess the estrogenic activity of chemicals. This technique is based on rainbow trout hepatocytes incubated in a basic medium free of any additional growth factors or estrogenic chemicals and uses the production of vitellogenin (VTG) as a marker for the estrogenic potency of the compounds tested. The system allows at least some of the metabolic transformations which are undertaken by the liver cells in vivo and could therefore be used for xenobiotic compounds which exhibit estrogenic activities after liver metabolic transformation. A dose-response curve was always consistently obtained using estradiol-17β (E2), with a mid point at around 100 nM E2 and a maximum response at around 1000 nM. Established estrogens such as 17 a 1 ethynylestradiol (EE2) or diethylstilboestrol (DES) were also tested. EE2 appeared to be equipotent with E2 and DES slightly less potent. E2 conjugates were, perhaps surprisingly, also very potent. Estradiol-3-sulfate was equipotent with E2 and estradiol-17β-glucuronide approx. 10% as potent. Other steroids such as androgens and progesterone, though active in the bioassay, were 3 orders of magnitude less potent than E2. Of the various steroids tested, only cortisol, at concentrations up to 50 μM, was completely inactive. Six different phytoestrogens were tested in the assay. All were weakly estrogenic, possessing approximately one thousanth the potency of E2 (they were as potent as the endrogens and progesterone). All six phytoestrogens, as well as the androgens and progesterone, were tested in the presence of tamoxifen. In all cases tamoxifen reduced the production of VTG significantly, demonstrating that the estrogenic action of all of these compounds was most likely mediated by the E2 receptor. The potencies determined here may not reflect the situation in vivo but can provide complementary results about the activity of chemicals which need an hepatic metabolization to be estrogenic. Hepatocyte cultures would profitably be developed in other species to sustain these results.
Url:
DOI: 10.1016/0960-0760(93)90086-C
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001397
- to stream Istex, to step Curation: 001395
- to stream Istex, to step Checkpoint: 001089
Links to Exploration step
ISTEX:BC82C0D67A25B0683FF26F965B03366DA4BFD76ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals</title>
<author><name sortKey="Pelissero, C" sort="Pelissero, C" uniqKey="Pelissero C" first="C." last="Pelissero">C. Pelissero</name>
</author>
<author><name sortKey="Flouriot, G" sort="Flouriot, G" uniqKey="Flouriot G" first="G." last="Flouriot">G. Flouriot</name>
</author>
<author><name sortKey="Foucher, J L" sort="Foucher, J L" uniqKey="Foucher J" first="J. L." last="Foucher">J. L. Foucher</name>
</author>
<author><name sortKey="Bennetau, B" sort="Bennetau, B" uniqKey="Bennetau B" first="B." last="Bennetau">B. Bennetau</name>
</author>
<author><name sortKey="Dunogues, J" sort="Dunogues, J" uniqKey="Dunogues J" first="J." last="Dunoguès">J. Dunoguès</name>
</author>
<author><name sortKey="Le Gac, F" sort="Le Gac, F" uniqKey="Le Gac F" first="F." last="Le Gac">F. Le Gac</name>
</author>
<author><name sortKey="Sumpter, J P" sort="Sumpter, J P" uniqKey="Sumpter J" first="J. P." last="Sumpter">J. P. Sumpter</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:BC82C0D67A25B0683FF26F965B03366DA4BFD76E</idno>
<date when="1993" year="1993">1993</date>
<idno type="doi">10.1016/0960-0760(93)90086-C</idno>
<idno type="url">https://api.istex.fr/document/BC82C0D67A25B0683FF26F965B03366DA4BFD76E/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001397</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001397</idno>
<idno type="wicri:Area/Istex/Curation">001395</idno>
<idno type="wicri:Area/Istex/Checkpoint">001089</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001089</idno>
<idno type="wicri:doubleKey">0960-0760:1993:Pelissero C:vitellogenin:synthesis:in</idno>
<idno type="wicri:Area/Main/Merge">001C80</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals</title>
<author><name sortKey="Pelissero, C" sort="Pelissero, C" uniqKey="Pelissero C" first="C." last="Pelissero">C. Pelissero</name>
<affiliation wicri:level="2"><country>Royaume-Uni</country>
<placeName><region type="country">Angleterre</region>
</placeName>
<wicri:cityArea>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Flouriot, G" sort="Flouriot, G" uniqKey="Flouriot G" first="G." last="Flouriot">G. Flouriot</name>
<affiliation wicri:level="4"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biologie Moléculaire Université de Rennes I, Campus de Beaulieu</wicri:regionArea>
<orgName type="university">Université de Rennes 1</orgName>
<placeName><settlement type="city">Rennes</settlement>
<region type="region" nuts="2">Région Bretagne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Foucher, J L" sort="Foucher, J L" uniqKey="Foucher J" first="J. L." last="Foucher">J. L. Foucher</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire INRA de Physiologie des Poissons, Campus de Rennes Beaulieu</wicri:regionArea>
<wicri:noRegion>Campus de Rennes Beaulieu</wicri:noRegion>
<wicri:noRegion>Campus de Rennes Beaulieu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bennetau, B" sort="Bennetau, B" uniqKey="Bennetau B" first="B." last="Bennetau">B. Bennetau</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Chimie Organique et Organométallique, URA 35 CNRS, Université de Bordeaux I, 351, Cours de la Libération</wicri:regionArea>
<wicri:noRegion>Cours de la Libération</wicri:noRegion>
<wicri:noRegion>Cours de la Libération</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dunogues, J" sort="Dunogues, J" uniqKey="Dunogues J" first="J." last="Dunoguès">J. Dunoguès</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Chimie Organique et Organométallique, URA 35 CNRS, Université de Bordeaux I, 351, Cours de la Libération</wicri:regionArea>
<wicri:noRegion>Cours de la Libération</wicri:noRegion>
<wicri:noRegion>Cours de la Libération</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Le Gac, F" sort="Le Gac, F" uniqKey="Le Gac F" first="F." last="Le Gac">F. Le Gac</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire INRA de Physiologie des Poissons, Campus de Rennes Beaulieu</wicri:regionArea>
<wicri:noRegion>Campus de Rennes Beaulieu</wicri:noRegion>
<wicri:noRegion>Campus de Rennes Beaulieu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sumpter, J P" sort="Sumpter, J P" uniqKey="Sumpter J" first="J. P." last="Sumpter">J. P. Sumpter</name>
<affiliation wicri:level="2"><country>Royaume-Uni</country>
<placeName><region type="country">Angleterre</region>
</placeName>
<wicri:cityArea>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Steroid Biochemistry and Molecular Biology</title>
<title level="j" type="abbrev">SBMB</title>
<idno type="ISSN">0960-0760</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1993">1993</date>
<biblScope unit="volume">44</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="263">263</biblScope>
<biblScope unit="page" to="272">272</biblScope>
</imprint>
<idno type="ISSN">0960-0760</idno>
</series>
<idno type="istex">BC82C0D67A25B0683FF26F965B03366DA4BFD76E</idno>
<idno type="DOI">10.1016/0960-0760(93)90086-C</idno>
<idno type="PII">0960-0760(93)90086-C</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0960-0760</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We describe here an in vitro technique to assess the estrogenic activity of chemicals. This technique is based on rainbow trout hepatocytes incubated in a basic medium free of any additional growth factors or estrogenic chemicals and uses the production of vitellogenin (VTG) as a marker for the estrogenic potency of the compounds tested. The system allows at least some of the metabolic transformations which are undertaken by the liver cells in vivo and could therefore be used for xenobiotic compounds which exhibit estrogenic activities after liver metabolic transformation. A dose-response curve was always consistently obtained using estradiol-17β (E2), with a mid point at around 100 nM E2 and a maximum response at around 1000 nM. Established estrogens such as 17 a 1 ethynylestradiol (EE2) or diethylstilboestrol (DES) were also tested. EE2 appeared to be equipotent with E2 and DES slightly less potent. E2 conjugates were, perhaps surprisingly, also very potent. Estradiol-3-sulfate was equipotent with E2 and estradiol-17β-glucuronide approx. 10% as potent. Other steroids such as androgens and progesterone, though active in the bioassay, were 3 orders of magnitude less potent than E2. Of the various steroids tested, only cortisol, at concentrations up to 50 μM, was completely inactive. Six different phytoestrogens were tested in the assay. All were weakly estrogenic, possessing approximately one thousanth the potency of E2 (they were as potent as the endrogens and progesterone). All six phytoestrogens, as well as the androgens and progesterone, were tested in the presence of tamoxifen. In all cases tamoxifen reduced the production of VTG significantly, demonstrating that the estrogenic action of all of these compounds was most likely mediated by the E2 receptor. The potencies determined here may not reflect the situation in vivo but can provide complementary results about the activity of chemicals which need an hepatic metabolization to be estrogenic. Hepatocyte cultures would profitably be developed in other species to sustain these results.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C80 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001C80 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:BC82C0D67A25B0683FF26F965B03366DA4BFD76E |texte= Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |