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DNA content in Eurasian sturgeon species determined by flow cytometry.

Identifieur interne : 001C77 ( Main/Merge ); précédent : 001C76; suivant : 001C78

DNA content in Eurasian sturgeon species determined by flow cytometry.

Auteurs : V J Birstein [Russie] ; A I Poletaev ; B F Goncharov

Source :

Cytometry [ 0196-4763 ] ; 1993.

RBID : pubmed:8513694

English descriptors

KwdEn :
- Animals, Asia, DNA (analysis), Europe, Fishes (classification), Fishes (genetics), Flow Cytometry, Polyploidy, Reference Standards, Species Specificity, Xenopus laevis (genetics).
MESH :
- chemical , analysis : DNA.
- geographic : Asia, Europe.
- classification : Fishes.
- genetics : Fishes, Xenopus laevis.
- Animals, Flow Cytometry, Polyploidy, Reference Standards, Species Specificity.

Abstract

The nuclear DNA content in 10 species of chondrostean fishes was measured by flow cytometry. The sterlet Acipenser ruthenus blood cells were used as an internal standard. The sterlet DNA content was calculated on the basis of comparison with the Xenopus laevis blood cells, 2C = 6.30 pg. In the tetraploid A. ruthenus and A. stellatus the DNA content comprises 3.74 pg/nucleus and is practically invariant; in Huso dauricus it is almost the same, 3.74-3.81 pg; and in A. nudiventris it is a little higher, 3.88-4.04 pg. In the oldest chondrostean, Pseudoscaphirhynchus kaufmanni, the nuclear DNA content is slightly lower, 2C = 3.46-3.48 pg, and in the American paddlefish Polyodon spathula it is lower still, 3.17 pg. In two octoploid sturgeons, A. baeri and A. gueldenstaedti, the DNA content is twice as high as that of the sterlet, 8.29-8.31 and 7.86-7.88 pg, respectively; a very similar amount, 8.24-8.42 pg, was determined in the hybrid Huso huso x A. ruthenus. In the Sakhalin sturgeon, A. medirostris (= A. mikadoi), the DNA content is two times higher than in the octoploids, 13.93-14.73 pg; therefore its ploidy may be 16n and the number of chromosomes could be 500.

DOI: 10.1002/cyto.990140406
PubMed: 8513694

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pubmed:8513694

Le document en format XML

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<author><name sortKey="Birstein, V J" sort="Birstein, V J" uniqKey="Birstein V" first="V J" last="Birstein">V J Birstein</name>
<affiliation wicri:level="3"><nlm:affiliation>N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow.</nlm:affiliation>
<country>Russie</country>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
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<wicri:orgArea>N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences</wicri:orgArea>
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<author><name sortKey="Poletaev, A I" sort="Poletaev, A I" uniqKey="Poletaev A" first="A I" last="Poletaev">A I Poletaev</name>
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<author><name sortKey="Goncharov, B F" sort="Goncharov, B F" uniqKey="Goncharov B" first="B F" last="Goncharov">B F Goncharov</name>
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<term>Fishes (genetics)</term>
<term>Flow Cytometry</term>
<term>Polyploidy</term>
<term>Reference Standards</term>
<term>Species Specificity</term>
<term>Xenopus laevis (genetics)</term>
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<term>Xenopus laevis</term>
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<front><div type="abstract" xml:lang="en">The nuclear DNA content in 10 species of chondrostean fishes was measured by flow cytometry. The sterlet Acipenser ruthenus blood cells were used as an internal standard. The sterlet DNA content was calculated on the basis of comparison with the Xenopus laevis blood cells, 2C = 6.30 pg. In the tetraploid A. ruthenus and A. stellatus the DNA content comprises 3.74 pg/nucleus and is practically invariant; in Huso dauricus it is almost the same, 3.74-3.81 pg; and in A. nudiventris it is a little higher, 3.88-4.04 pg. In the oldest chondrostean, Pseudoscaphirhynchus kaufmanni, the nuclear DNA content is slightly lower, 2C = 3.46-3.48 pg, and in the American paddlefish Polyodon spathula it is lower still, 3.17 pg. In two octoploid sturgeons, A. baeri and A. gueldenstaedti, the DNA content is twice as high as that of the sterlet, 8.29-8.31 and 7.86-7.88 pg, respectively; a very similar amount, 8.24-8.42 pg, was determined in the hybrid Huso huso x A. ruthenus. In the Sakhalin sturgeon, A. medirostris (= A. mikadoi), the DNA content is two times higher than in the octoploids, 13.93-14.73 pg; therefore its ploidy may be 16n and the number of chromosomes could be 500.</div>
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DNA content in Eurasian sturgeon species determined by flow cytometry.

DNA content in Eurasian sturgeon species determined by flow cytometry.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki