Ethidium Bromide Nuclear Staining and Fluorescence Microscopy: An Alternative Method for Triploidy Detection in Fish
Identifieur interne : 001A75 ( Main/Merge ); précédent : 001A74; suivant : 001A76Ethidium Bromide Nuclear Staining and Fluorescence Microscopy: An Alternative Method for Triploidy Detection in Fish
Auteurs : Suttiporn Thititananukiji [Thaïlande] ; Renu Vejaratpimol [Thaïlande] ; Thanit Pewnim [Thaïlande] ; Arlo W. Fast [États-Unis]Source :
- Journal of the World Aquaculture Society [ 0893-8849 ] ; 1996-06.
Abstract
Abstract.– Erythrocyte nuclei from normal diploid (2N) and cold‐shocked triploid (3N) Asian catfish Clarias macrocephalus were comparatively stained with fluorescent stain, ethidium bromide and with a bright‐field stain, Giemsa. Stains were applied to dried blood smears on glass slides, with blood taken from fingerling fish without sacrificing the fish. Both stains produced comparable quality visual comparisons, with significant differences (P < 0.001) between nuclear dimensions of 2N and 3N cells. Triploid nuclei were larger and more elongate than diploid nuclei. Karyotypic comparisons revealed that diploid fish had 54 chromosomes, while triploid fish had 81. Ethidium bromide nuclear staining required only 3 min after slides were air‐dried and fixed, while Giemsa staining required more than 50 min.
Url:
DOI: 10.1111/j.1749-7345.1996.tb00272.x
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ethidium Bromide Nuclear Staining and Fluorescence Microscopy: An Alternative Method for Triploidy Detection in Fish</title><author><name sortKey="Thititananukiji, Suttiporn" sort="Thititananukiji, Suttiporn" uniqKey="Thititananukiji S" first="Suttiporn" last="Thititananukiji">Suttiporn Thititananukiji</name></author><author><name sortKey="Vejaratpimol, Renu" sort="Vejaratpimol, Renu" uniqKey="Vejaratpimol R" first="Renu" last="Vejaratpimol">Renu Vejaratpimol</name></author><author><name sortKey="Pewnim, Thanit" sort="Pewnim, Thanit" uniqKey="Pewnim T" first="Thanit" last="Pewnim">Thanit Pewnim</name></author><author><name sortKey="Fast, Arlo W" sort="Fast, Arlo W" uniqKey="Fast A" first="Arlo W." last="Fast">Arlo W. Fast</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D98B368F3D9BBB202FE8B08E090F46BAA794381B</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1111/j.1749-7345.1996.tb00272.x</idno><idno type="url">https://api.istex.fr/document/D98B368F3D9BBB202FE8B08E090F46BAA794381B/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000275</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000275</idno><idno type="wicri:Area/Istex/Curation">000275</idno><idno type="wicri:Area/Istex/Checkpoint">000F63</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F63</idno><idno type="wicri:doubleKey">0893-8849:1996:Thititananukiji S:ethidium:bromide:nuclear</idno><idno type="wicri:Area/Main/Merge">001A75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Ethidium Bromide Nuclear Staining and Fluorescence Microscopy: An Alternative Method for Triploidy Detection in Fish</title><author><name sortKey="Thititananukiji, Suttiporn" sort="Thititananukiji, Suttiporn" uniqKey="Thititananukiji S" first="Suttiporn" last="Thititananukiji">Suttiporn Thititananukiji</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biology, Silpakorn University</wicri:regionArea><wicri:noRegion>Silpakorn University</wicri:noRegion></affiliation></author><author><name sortKey="Vejaratpimol, Renu" sort="Vejaratpimol, Renu" uniqKey="Vejaratpimol R" first="Renu" last="Vejaratpimol">Renu Vejaratpimol</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biology, Silpakorn University</wicri:regionArea><wicri:noRegion>Silpakorn University</wicri:noRegion></affiliation></author><author><name sortKey="Pewnim, Thanit" sort="Pewnim, Thanit" uniqKey="Pewnim T" first="Thanit" last="Pewnim">Thanit Pewnim</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Chemistry, Silpakorn University</wicri:regionArea><wicri:noRegion>Silpakorn University</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Fast, Arlo W" sort="Fast, Arlo W" uniqKey="Fast A" first="Arlo W." last="Fast">Arlo W. Fast</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Hawaï</region></placeName><wicri:cityArea>Hawaii Institute of Marine Biology, University of Hawaii at Manoa, Honolulu</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of the World Aquaculture Society</title><idno type="ISSN">0893-8849</idno><idno type="eISSN">1749-7345</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1996-06">1996-06</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="213">213</biblScope><biblScope unit="page" to="217">217</biblScope></imprint><idno type="ISSN">0893-8849</idno></series><idno type="istex">D98B368F3D9BBB202FE8B08E090F46BAA794381B</idno><idno type="DOI">10.1111/j.1749-7345.1996.tb00272.x</idno><idno type="ArticleID">JWAS213</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-8849</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Abstract.– Erythrocyte nuclei from normal diploid (2N) and cold‐shocked triploid (3N) Asian catfish Clarias macrocephalus were comparatively stained with fluorescent stain, ethidium bromide and with a bright‐field stain, Giemsa. Stains were applied to dried blood smears on glass slides, with blood taken from fingerling fish without sacrificing the fish. Both stains produced comparable quality visual comparisons, with significant differences (P < 0.001) between nuclear dimensions of 2N and 3N cells. Triploid nuclei were larger and more elongate than diploid nuclei. Karyotypic comparisons revealed that diploid fish had 54 chromosomes, while triploid fish had 81. Ethidium bromide nuclear staining required only 3 min after slides were air‐dried and fixed, while Giemsa staining required more than 50 min.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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