Effects of Incubation Media on Maturation of Isolated Ovarian Follicles of Siberian Sturgeon ( Acipenser baeri Brandt) Induced by Sturgeon Gonadotropic Preparation or 17 α ,20 β -Dihydroxyprogesterone
Identifieur interne : 001960 ( Main/Merge ); précédent : 001959; suivant : 001961Effects of Incubation Media on Maturation of Isolated Ovarian Follicles of Siberian Sturgeon ( Acipenser baeri Brandt) Induced by Sturgeon Gonadotropic Preparation or 17 α ,20 β -Dihydroxyprogesterone
Auteurs : Patrick Williot [France]Source :
- Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology [ 0742-8413 ] ; 1997.
English descriptors
- KwdEn :
Abstract
In vitro maturation of Siberian sturgeon follicles was studied following induction with sturgeon gonadotropic preparations (aci-GTH, ED50) or 17α,20β-diOHP (ET50 and complete maturation (GVBD)). aci-GTH ED50 varied greatly, over an order of magnitude depending on female or incubation medium. Spontaneous maturation was recorded at different levels, for different females and in different media probably as a result of a peculiar situation between follicle state and medium. Analysis of variance demonstrated the great importance of the medium and its interaction with the follicle. The best preferred incubation medium is based on Siberian sturgeon plasma characteristics because it provides good discrimination of the female physiological state and it avoids “abnormal” responses. The optima for pH and osmotic pressure are approximately pH 8 and 270–280 mosmol/l, respectively, although there were great individual variations of 17α,20β-diOHP-dependent GVBD to pH and osmotic pressure. The 17α,20β-diOHP ET50 is incubation medium–independent and readily discriminates the females. Some other observations are discussed in the light of the literature.
Url:
DOI: 10.1016/S0742-8413(97)00178-3
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A45
- to stream Istex, to step Curation: 000A43
- to stream Istex, to step Checkpoint: 000F12
Links to Exploration step
ISTEX:46EA0CA688187BD127E06C63D77C16A523562A70Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of Incubation Media on Maturation of Isolated Ovarian Follicles of Siberian Sturgeon ( Acipenser baeri Brandt) Induced by Sturgeon Gonadotropic Preparation or 17 α ,20 β -Dihydroxyprogesterone</title>
<author><name sortKey="Williot, P" sort="Williot, P" uniqKey="Williot P" first="P." last="Williot">Patrick Williot</name>
<affiliation><country>France</country>
<placeName><settlement type="city">Bordeaux</settlement>
<settlement type="town">Cestas</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="laboratoire" n="5">Cemagref - centre de Bordeaux</orgName>
<orgName type="institution">Centre national du machinisme agricole, du génie rural, des eaux et des forêts</orgName>
<orgName type="institution">Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:46EA0CA688187BD127E06C63D77C16A523562A70</idno>
<date when="1997" year="1997">1997</date>
<idno type="doi">10.1016/S0742-8413(97)00178-3</idno>
<idno type="url">https://api.istex.fr/document/46EA0CA688187BD127E06C63D77C16A523562A70/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000A45</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A45</idno>
<idno type="wicri:Area/Istex/Curation">000A43</idno>
<idno type="wicri:Area/Istex/Checkpoint">000F12</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F12</idno>
<idno type="wicri:doubleKey">0742-8413:1997:Williot P:effects:of:incubation</idno>
<idno type="wicri:Area/Main/Merge">001960</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Effects of Incubation Media on Maturation of Isolated Ovarian Follicles of Siberian Sturgeon ( Acipenser baeri Brandt) Induced by Sturgeon Gonadotropic Preparation or 17 α ,20 β -Dihydroxyprogesterone</title>
<author><name sortKey="Williot, P" sort="Williot, P" uniqKey="Williot P" first="P." last="Williot">Patrick Williot</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>Ressources Aquatiques Continentales, Cemagref, Cestas</wicri:regionArea>
<wicri:noRegion>Cestas</wicri:noRegion>
<wicri:noRegion>Cestas</wicri:noRegion>
<placeName><settlement type="city">Bordeaux</settlement>
<settlement type="town">Cestas</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="laboratoire" n="5">Cemagref - centre de Bordeaux</orgName>
<orgName type="institution">Centre national du machinisme agricole, du génie rural, des eaux et des forêts</orgName>
<orgName type="institution">Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture</orgName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title>
<title level="j" type="abbrev">CBCOLD</title>
<idno type="ISSN">0742-8413</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1997">1997</date>
<biblScope unit="volume">118</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="285">285</biblScope>
<biblScope unit="page" to="293">293</biblScope>
</imprint>
<idno type="ISSN">0742-8413</idno>
</series>
<idno type="istex">46EA0CA688187BD127E06C63D77C16A523562A70</idno>
<idno type="DOI">10.1016/S0742-8413(97)00178-3</idno>
<idno type="PII">S0742-8413(97)00178-3</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0742-8413</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>17α,20β-dihydroxyprogesterone</term>
<term>Acipenser baeri</term>
<term>gonadotropic preparations</term>
<term>incubation medium</term>
<term>maturation</term>
<term>osmotic pressure</term>
<term>ovarian follicles</term>
<term>pH</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In vitro maturation of Siberian sturgeon follicles was studied following induction with sturgeon gonadotropic preparations (aci-GTH, ED50) or 17α,20β-diOHP (ET50 and complete maturation (GVBD)). aci-GTH ED50 varied greatly, over an order of magnitude depending on female or incubation medium. Spontaneous maturation was recorded at different levels, for different females and in different media probably as a result of a peculiar situation between follicle state and medium. Analysis of variance demonstrated the great importance of the medium and its interaction with the follicle. The best preferred incubation medium is based on Siberian sturgeon plasma characteristics because it provides good discrimination of the female physiological state and it avoids “abnormal” responses. The optima for pH and osmotic pressure are approximately pH 8 and 270–280 mosmol/l, respectively, although there were great individual variations of 17α,20β-diOHP-dependent GVBD to pH and osmotic pressure. The 17α,20β-diOHP ET50 is incubation medium–independent and readily discriminates the females. Some other observations are discussed in the light of the literature.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001960 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001960 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:46EA0CA688187BD127E06C63D77C16A523562A70 |texte= Effects of Incubation Media on Maturation of Isolated Ovarian Follicles of Siberian Sturgeon ( Acipenser baeri Brandt) Induced by Sturgeon Gonadotropic Preparation or 17 α ,20 β -Dihydroxyprogesterone }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |