Induced Meiotic Gynogenesis of Paddlefish Polyodon spathula
Identifieur interne : 001955 ( Main/Merge ); précédent : 001954; suivant : 001956Induced Meiotic Gynogenesis of Paddlefish Polyodon spathula
Auteurs : Steven D. Mims [États-Unis] ; William L. Shelton [États-Unis] ; Otomar Linhart [République tchèque] ; Changzheng Wang [États-Unis]Source :
- Journal of the World Aquaculture Society [ 0893-8849 ] ; 1997-12.
Abstract
Viable, diploid gynogenetic (gynogenotes) paddlefish Polyodon spathula were produced by activating eggs with ultraviolet‐irradiated shovelnose sturgeon Scaphirhynchus platorynchus spermatozoa and heat shocking. Without irradiation treatment, sturgeon spermatozoa appeared to activate the eggs (up to gastrulation), but did not result in any viable hybrids. Experiment 1 determined that heat‐shock treatment of 35 C for a 2‐min duration within the interval of 2–22 min post‐activation resulted in highest yield of gynogenotes (12–19%) from eggs incubated at 18 C. Experiment 2 applied the heat shock treatment at 35 C from 14.0 to 28.0 min in 2‐min intervals after activation at 18 C for a larger scale of gynogenetic production. Both experiments showed that the best yields of gynogenotes were obtained when the heat shock treatment occurred at 16, 18, and 20 min after activation. When these times were expressed in terms of τ0. units (duration of one mitotic cycle of synchronous cell division related to water temperatures), optimal activations were 0.26, 0.29, and 0.32τ0 (τ0@ 18 C = 63.5 min). Experiment 3 tested the utility of τ0. at two different pre‐shock incubation water temperatures of 18 C and 16 C, and determined that there was no significant interaction in percentage of viable gynogenotes between two different incubation temperatures and the mitotic intervals (0.21, 0.26, 0.31, 0.36, 0.41τ0) tested. Best survival of gynogenotes occurred when eggs held at either pre‐shock incubation water temperatures were shocked at 0.26τ0 All gynogenotes examined were histologically confirmed to have ovarian tissue and were determined to have similar oocyte development to that of normal female (control) paddlefish.
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DOI: 10.1111/j.1749-7345.1997.tb00280.x
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Induced Meiotic Gynogenesis of Paddlefish Polyodon spathula</title><author><name sortKey="Mims, Steven D" sort="Mims, Steven D" uniqKey="Mims S" first="Steven D." last="Mims">Steven D. Mims</name></author><author><name sortKey="Shelton, William L" sort="Shelton, William L" uniqKey="Shelton W" first="William L." last="Shelton">William L. Shelton</name></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name></author><author><name sortKey="Wang, Changzheng" sort="Wang, Changzheng" uniqKey="Wang C" first="Changzheng" last="Wang">Changzheng Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CBB29D99E2A32C46A3E03C50141C19417145BA29</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1111/j.1749-7345.1997.tb00280.x</idno><idno type="url">https://api.istex.fr/document/CBB29D99E2A32C46A3E03C50141C19417145BA29/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001531</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001531</idno><idno type="wicri:Area/Istex/Curation">001529</idno><idno type="wicri:Area/Istex/Checkpoint">000F07</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F07</idno><idno type="wicri:doubleKey">0893-8849:1997:Mims S:induced:meiotic:gynogenesis</idno><idno type="wicri:Area/Main/Merge">001955</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Induced Meiotic Gynogenesis of Paddlefish Polyodon spathula</title><author><name sortKey="Mims, Steven D" sort="Mims, Steven D" uniqKey="Mims S" first="Steven D." last="Mims">Steven D. Mims</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Kentucky</region></placeName><wicri:cityArea>Aquaculture Research Center, Kentucky State University, Frankfort</wicri:cityArea></affiliation></author><author><name sortKey="Shelton, William L" sort="Shelton, William L" uniqKey="Shelton W" first="William L." last="Shelton">William L. Shelton</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Oklahoma</region></placeName><wicri:cityArea>Zoology Department, University of Oklahoma, Norman</wicri:cityArea></affiliation></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, 38925 Vodnany</wicri:regionArea><wicri:noRegion>38925 Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Changzheng" sort="Wang, Changzheng" uniqKey="Wang C" first="Changzheng" last="Wang">Changzheng Wang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Kentucky</region></placeName><wicri:cityArea>Community Research Service, Kentucky State University, Frankfort</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of the World Aquaculture Society</title><idno type="ISSN">0893-8849</idno><idno type="eISSN">1749-7345</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1997-12">1997-12</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="334">334</biblScope><biblScope unit="page" to="343">343</biblScope></imprint><idno type="ISSN">0893-8849</idno></series><idno type="istex">CBB29D99E2A32C46A3E03C50141C19417145BA29</idno><idno type="DOI">10.1111/j.1749-7345.1997.tb00280.x</idno><idno type="ArticleID">JWAS334</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-8849</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Viable, diploid gynogenetic (gynogenotes) paddlefish Polyodon spathula were produced by activating eggs with ultraviolet‐irradiated shovelnose sturgeon Scaphirhynchus platorynchus spermatozoa and heat shocking. Without irradiation treatment, sturgeon spermatozoa appeared to activate the eggs (up to gastrulation), but did not result in any viable hybrids. Experiment 1 determined that heat‐shock treatment of 35 C for a 2‐min duration within the interval of 2–22 min post‐activation resulted in highest yield of gynogenotes (12–19%) from eggs incubated at 18 C. Experiment 2 applied the heat shock treatment at 35 C from 14.0 to 28.0 min in 2‐min intervals after activation at 18 C for a larger scale of gynogenetic production. Both experiments showed that the best yields of gynogenotes were obtained when the heat shock treatment occurred at 16, 18, and 20 min after activation. When these times were expressed in terms of τ0. units (duration of one mitotic cycle of synchronous cell division related to water temperatures), optimal activations were 0.26, 0.29, and 0.32τ0 (τ0@ 18 C = 63.5 min). Experiment 3 tested the utility of τ0. at two different pre‐shock incubation water temperatures of 18 C and 16 C, and determined that there was no significant interaction in percentage of viable gynogenotes between two different incubation temperatures and the mitotic intervals (0.21, 0.26, 0.31, 0.36, 0.41τ0) tested. Best survival of gynogenotes occurred when eggs held at either pre‐shock incubation water temperatures were shocked at 0.26τ0 All gynogenotes examined were histologically confirmed to have ovarian tissue and were determined to have similar oocyte development to that of normal female (control) paddlefish.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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