Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines.
Identifieur interne : 001829 ( Main/Merge ); précédent : 001828; suivant : 001830Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines.
Auteurs : L R Watson [États-Unis] ; J M Groff ; R P HedrickSource :
- Diseases of aquatic organisms [ 0177-5103 ] ; 1998.
English descriptors
- KwdEn :
- Animals, Cell Line, Cytopathogenic Effect, Viral, DNA Virus Infections (veterinary), DNA Virus Infections (virology), Esophagus (pathology), Esophagus (virology), Fish Diseases (virology), Fishes, Gills (pathology), Gills (virology), Iridovirus (physiology), Iridovirus (ultrastructure), Liver (pathology), Liver (virology), Microscopy, Electron, Sense Organs (virology), Skin (pathology), Skin (virology), Virion (physiology), Virion (ultrastructure), Virus Replication.
- MESH :
- pathology : Esophagus, Gills, Liver, Skin.
- physiology : Iridovirus, Virion.
- ultrastructure : Iridovirus, Virion.
- veterinary : DNA Virus Infections.
- virology : DNA Virus Infections, Esophagus, Fish Diseases, Gills, Liver, Sense Organs, Skin.
- Animals, Cell Line, Cytopathogenic Effect, Viral, Fishes, Microscopy, Electron, Virus Replication.
Abstract
Characteristics of the in vitro propagation of the white sturgeon iridovirus (WSIV) were examined in 6 sturgeon cell lines. One new cell line originating from gonadal tissues (WSGO) produced up to 12-fold more WSIV [approximately 22 TCID50 (50% tissue culture infective dose) cell-1], than that of a previously established reference spleen cell line (WSS-2). Infected WSGO cell cultures were examined using phase microscopy, viral infectivity assay and transmission electron microscopy (TEM). At 15 degrees C, both mature virions and infectious virus were first detected after 7 d post-infection. Capsids acquired envelopes in the cytoplasm and virions remained primarily cell-associated during the 35 d replication cycle. Cellular changes including hyper-refractility and cytoplasmic swelling with dense cytoplasmic inclusions correlated to extensive proliferation of cytoplasmic vesicles and viral assembly sites. These cytological characteristics corresponded to changes in target cells of WSIV-infected juvenile white sturgeon following bath challenge. Microscopic changes in stained tissue sections of the host epithelium were detected 4 d post-challenge, approximately 8 d prior to the onset of clinical signs. Hypertrophied Malpighian cells surrounded by a prominent pericellular cisternum characterized epithelial lesions in the skin. Similar changes to epithelial cells of the barbels, olfactory organs and esophagus were also observed. Destruction of the sensory epithelium is suggested as a cause for cessation of feeding which occurs early in the infection of white sturgeon juveniles with WSIV.
DOI: 10.3354/dao032173
PubMed: 9676244
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000671
- to stream PubMed, to step Curation: 000671
- to stream PubMed, to step Checkpoint: 000671
- to stream Ncbi, to step Merge: 000899
- to stream Ncbi, to step Curation: 000899
- to stream Ncbi, to step Checkpoint: 000899
Links to Exploration step
pubmed:9676244Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines.</title>
<author><name sortKey="Watson, L R" sort="Watson, L R" uniqKey="Watson L" first="L R" last="Watson">L R Watson</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616</wicri:regionArea>
<wicri:noRegion>Davis 95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Groff, J M" sort="Groff, J M" uniqKey="Groff J" first="J M" last="Groff">J M Groff</name>
</author>
<author><name sortKey="Hedrick, R P" sort="Hedrick, R P" uniqKey="Hedrick R" first="R P" last="Hedrick">R P Hedrick</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1998">1998</date>
<idno type="RBID">pubmed:9676244</idno>
<idno type="pmid">9676244</idno>
<idno type="doi">10.3354/dao032173</idno>
<idno type="wicri:Area/PubMed/Corpus">000671</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000671</idno>
<idno type="wicri:Area/PubMed/Curation">000671</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000671</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000671</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000671</idno>
<idno type="wicri:Area/Ncbi/Merge">000899</idno>
<idno type="wicri:Area/Ncbi/Curation">000899</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000899</idno>
<idno type="wicri:doubleKey">0177-5103:1998:Watson L:replication:and:pathogenesis</idno>
<idno type="wicri:Area/Main/Merge">001829</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines.</title>
<author><name sortKey="Watson, L R" sort="Watson, L R" uniqKey="Watson L" first="L R" last="Watson">L R Watson</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616</wicri:regionArea>
<wicri:noRegion>Davis 95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Groff, J M" sort="Groff, J M" uniqKey="Groff J" first="J M" last="Groff">J M Groff</name>
</author>
<author><name sortKey="Hedrick, R P" sort="Hedrick, R P" uniqKey="Hedrick R" first="R P" last="Hedrick">R P Hedrick</name>
</author>
</analytic>
<series><title level="j">Diseases of aquatic organisms</title>
<idno type="ISSN">0177-5103</idno>
<imprint><date when="1998" type="published">1998</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cytopathogenic Effect, Viral</term>
<term>DNA Virus Infections (veterinary)</term>
<term>DNA Virus Infections (virology)</term>
<term>Esophagus (pathology)</term>
<term>Esophagus (virology)</term>
<term>Fish Diseases (virology)</term>
<term>Fishes</term>
<term>Gills (pathology)</term>
<term>Gills (virology)</term>
<term>Iridovirus (physiology)</term>
<term>Iridovirus (ultrastructure)</term>
<term>Liver (pathology)</term>
<term>Liver (virology)</term>
<term>Microscopy, Electron</term>
<term>Sense Organs (virology)</term>
<term>Skin (pathology)</term>
<term>Skin (virology)</term>
<term>Virion (physiology)</term>
<term>Virion (ultrastructure)</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Esophagus</term>
<term>Gills</term>
<term>Liver</term>
<term>Skin</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Iridovirus</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Iridovirus</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>DNA Virus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>DNA Virus Infections</term>
<term>Esophagus</term>
<term>Fish Diseases</term>
<term>Gills</term>
<term>Liver</term>
<term>Sense Organs</term>
<term>Skin</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Fishes</term>
<term>Microscopy, Electron</term>
<term>Virus Replication</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Characteristics of the in vitro propagation of the white sturgeon iridovirus (WSIV) were examined in 6 sturgeon cell lines. One new cell line originating from gonadal tissues (WSGO) produced up to 12-fold more WSIV [approximately 22 TCID50 (50% tissue culture infective dose) cell-1], than that of a previously established reference spleen cell line (WSS-2). Infected WSGO cell cultures were examined using phase microscopy, viral infectivity assay and transmission electron microscopy (TEM). At 15 degrees C, both mature virions and infectious virus were first detected after 7 d post-infection. Capsids acquired envelopes in the cytoplasm and virions remained primarily cell-associated during the 35 d replication cycle. Cellular changes including hyper-refractility and cytoplasmic swelling with dense cytoplasmic inclusions correlated to extensive proliferation of cytoplasmic vesicles and viral assembly sites. These cytological characteristics corresponded to changes in target cells of WSIV-infected juvenile white sturgeon following bath challenge. Microscopic changes in stained tissue sections of the host epithelium were detected 4 d post-challenge, approximately 8 d prior to the onset of clinical signs. Hypertrophied Malpighian cells surrounded by a prominent pericellular cisternum characterized epithelial lesions in the skin. Similar changes to epithelial cells of the barbels, olfactory organs and esophagus were also observed. Destruction of the sensory epithelium is suggested as a cause for cessation of feeding which occurs early in the infection of white sturgeon juveniles with WSIV.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001829 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001829 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:9676244 |texte= Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:9676244" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |