Microsatellite analysis of genetic variation in sturgeon: New primer sequences for Scaphirhynchus and Acipenser
Identifieur interne : 001663 ( Main/Merge ); précédent : 001662; suivant : 001664Microsatellite analysis of genetic variation in sturgeon: New primer sequences for Scaphirhynchus and Acipenser
Auteurs : Eve C. Mcquown [États-Unis] ; Brian L. Sloss [États-Unis] ; Robert J. Sheehan [États-Unis] ; Jeff Rodzen [États-Unis] ; Gregory J. Tranah [États-Unis] ; Bernie May [États-Unis]Source :
- Transactions of the American Fisheries Society : (1900) [ 0002-8487 ] ; 2000.
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- Pascal (Inist)
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Abstract
Low levels of genetic variation at traditional molecular markers have hampered genetic research within the family Acipenseridae. In an effort to develop a large set of polymorphic genetic markers, 172 clones were sequenced from three subgenomic libraries of shovelnose sturgeon Scaphirhynchus platorynchus; the libraries were enriched for two dinucleotide and one tetranucleotide microsatellite motifs (CA, GA, and TAGA). Primers were designed for 113 of the sequences and tested against shovelnose sturgeon, pallid sturgeon S. albus, white sturgeon Acipenser transmontanus, lake sturgeon A. fulvescens, and green sturgeon A. medirostris. Of the 113 primer sets tested, 96% amplified in one or more species (58 dimeric and 50 tetrameric). In Scaphirhynchus species, 93% of all loci amplified, and 76% were polymorphic. Within the individual Acipenser species, 65-80% of loci amplified, with 42-58% being polymorphic. Polymorphic systems for Scaphirhynchus species predominately displayed simple, disomic banding patterns, while those for Acipenser species typically displayed banding patterns characteristic of tetraploid or higher polyploid levels. These new microsatellite loci provide a group of genetic markers that are detectable with noninvasive sampling and that should prove useful in the preservation of threatened and endangered sturgeon species worldwide.
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Mcquown</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Animal Science, Meyer Hall, University of California</s1><s2>Davis, California 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Davis, California 95616</wicri:noRegion></affiliation></author><author><name sortKey="Sloss, Brian L" sort="Sloss, Brian L" uniqKey="Sloss B" first="Brian L." last="Sloss">Brian L. Sloss</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Fisheries and Illinois Aquaculture Center, Department of Zoology, Southern Illinois University</s1><s2>Carbondale, Illinois 62901-6511</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Carbondale, Illinois 62901-6511</wicri:noRegion></affiliation></author><author><name sortKey="Sheehan, Robert J" sort="Sheehan, Robert J" uniqKey="Sheehan R" first="Robert J." last="Sheehan">Robert J. Sheehan</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Fisheries and Illinois Aquaculture Center, Department of Zoology, Southern Illinois University</s1><s2>Carbondale, Illinois 62901-6511</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Carbondale, Illinois 62901-6511</wicri:noRegion></affiliation></author><author><name sortKey="Rodzen, Jeff" sort="Rodzen, Jeff" uniqKey="Rodzen J" first="Jeff" last="Rodzen">Jeff Rodzen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Animal Science, Meyer Hall, University of California</s1><s2>Davis, California 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Davis, California 95616</wicri:noRegion></affiliation></author><author><name sortKey="Tranah, Gregory J" sort="Tranah, Gregory J" uniqKey="Tranah G" first="Gregory J." last="Tranah">Gregory J. Tranah</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Animal Science, Meyer Hall, University of California</s1><s2>Davis, California 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Davis, California 95616</wicri:noRegion></affiliation></author><author><name sortKey="May, Bernie" sort="May, Bernie" uniqKey="May B" first="Bernie" last="May">Bernie May</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Animal Science, Meyer Hall, University of California</s1><s2>Davis, California 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Davis, California 95616</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Natural Resources, Fernow Hall, Cornell University</s1><s2>Ithaca, New York 14853</s2><s3>USA</s3><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Ithaca (New York)</settlement><region type="state">État de New York</region></placeName><orgName type="university">Université Cornell</orgName></affiliation></author></analytic><series><title level="j" type="main">Transactions of the American Fisheries Society : (1900)</title><title level="j" type="abbreviated">Trans. Am. Fish. Soc. : (1900)</title><idno type="ISSN">0002-8487</idno><imprint><date when="2000">2000</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Transactions of the American Fisheries Society : (1900)</title><title level="j" type="abbreviated">Trans. Am. Fish. Soc. : (1900)</title><idno type="ISSN">0002-8487</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term><term>Endangered species</term><term>Gene amplification</term><term>Genetic marker</term><term>Genetic stock</term><term>Genetic variability</term><term>Interspecific comparison</term><term>Microsatellite DNA</term><term>Polymorphism</term><term>Polyploidy</term><term>Population exploitation</term><term>Primer</term><term>Resource management</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Marqueur génétique</term><term>DNA microsatellite</term><term>Variabilité génétique</term><term>Polyploïdie</term><term>Polymorphisme</term><term>Stock génétique</term><term>Gestion ressources</term><term>Espèce menacée</term><term>Exploitation population</term><term>Amorce</term><term>Amplification génique</term><term>Comparaison interspécifique</term><term>Acipenser transmontanus</term><term>Acipenser</term><term>Scaphirhynchus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Low levels of genetic variation at traditional molecular markers have hampered genetic research within the family Acipenseridae. In an effort to develop a large set of polymorphic genetic markers, 172 clones were sequenced from three subgenomic libraries of shovelnose sturgeon Scaphirhynchus platorynchus; the libraries were enriched for two dinucleotide and one tetranucleotide microsatellite motifs (CA, GA, and TAGA). Primers were designed for 113 of the sequences and tested against shovelnose sturgeon, pallid sturgeon S. albus, white sturgeon Acipenser transmontanus, lake sturgeon A. fulvescens, and green sturgeon A. medirostris. Of the 113 primer sets tested, 96% amplified in one or more species (58 dimeric and 50 tetrameric). In Scaphirhynchus species, 93% of all loci amplified, and 76% were polymorphic. Within the individual Acipenser species, 65-80% of loci amplified, with 42-58% being polymorphic. Polymorphic systems for Scaphirhynchus species predominately displayed simple, disomic banding patterns, while those for Acipenser species typically displayed banding patterns characteristic of tetraploid or higher polyploid levels. These new microsatellite loci provide a group of genetic markers that are detectable with noninvasive sampling and that should prove useful in the preservation of threatened and endangered sturgeon species worldwide.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Mcquown, Eve C" sort="Mcquown, Eve C" uniqKey="Mcquown E" first="Eve C." last="Mcquown">Eve C. Mcquown</name></noRegion><name sortKey="May, Bernie" sort="May, Bernie" uniqKey="May B" first="Bernie" last="May">Bernie May</name><name sortKey="May, Bernie" sort="May, Bernie" uniqKey="May B" first="Bernie" last="May">Bernie May</name><name sortKey="Rodzen, Jeff" sort="Rodzen, Jeff" uniqKey="Rodzen J" first="Jeff" last="Rodzen">Jeff Rodzen</name><name sortKey="Sheehan, Robert J" sort="Sheehan, Robert J" uniqKey="Sheehan R" first="Robert J." last="Sheehan">Robert J. Sheehan</name><name sortKey="Sloss, Brian L" sort="Sloss, Brian L" uniqKey="Sloss B" first="Brian L." last="Sloss">Brian L. Sloss</name><name sortKey="Tranah, Gregory J" sort="Tranah, Gregory J" uniqKey="Tranah G" first="Gregory J." last="Tranah">Gregory J. Tranah</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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