EsturgeonV1, Main, Merge, bibRecord, 001454

Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

Identifieur interne : 001454 ( Main/Merge ); précédent : 001453; suivant : 001455

Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

Auteurs : J. Glogowski [Pologne] ; R. Kolman [Pologne] ; M. Szczepkowski [Pologne] ; A. Horvath [Hongrie] ; B. Urbanyi [Hongrie] ; P. Sieczynski [Pologne] ; A. Rzemieniecki [Pologne] ; J. Domagala [Pologne] ; W. Demianowicz [Pologne] ; R. Kowalski [Pologne] ; A. Ciereszko [Pologne]

Source :

Aquaculture : (Amsterdam) [ 0044-8486 ] ; 2002.

RBID : Pascal:02-0494829

Descripteurs français

Pascal (Inist)
- Mâle, Sperme, Cryoconservation, Méthanol, Cryoprotecteur, Succès reproducteur, Pisciculture, Pologne, Acipenser baeri.
Wicri :
- geographic : Pologne.
- topic : Méthanol, Pisciculture.

English descriptors

KwdEn :
- Acipenser baeri, Breeding success, Cryopreservation, Cryoprotective agent, Male, Methanol, Pisciculture, Poland, Semen.

Abstract

Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris-sucrose-KCI (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris-Nacl (10 mM Tris, 25 mM NaCI, pH 8.5), and Tris-sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris-sucrose-KCI, Tris-NaCI, and Tris-sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris-sucrose-KCl and Tris-NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.

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to stream PascalFrancis, to step Corpus: 000306
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Pascal:02-0494829

Le document en format XML

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<author><name sortKey="Rzemieniecki, A" sort="Rzemieniecki, A" uniqKey="Rzemieniecki A" first="A." last="Rzemieniecki">A. Rzemieniecki</name>
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<author><name sortKey="Domagala, J" sort="Domagala, J" uniqKey="Domagala J" first="J." last="Domagala">J. Domagala</name>
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<series><title level="j" type="main">Aquaculture : (Amsterdam)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser baeri</term>
<term>Breeding success</term>
<term>Cryopreservation</term>
<term>Cryoprotective agent</term>
<term>Male</term>
<term>Methanol</term>
<term>Pisciculture</term>
<term>Poland</term>
<term>Semen</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Mâle</term>
<term>Sperme</term>
<term>Cryoconservation</term>
<term>Méthanol</term>
<term>Cryoprotecteur</term>
<term>Succès reproducteur</term>
<term>Pisciculture</term>
<term>Pologne</term>
<term>Acipenser baeri</term>
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<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Pologne</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Méthanol</term>
<term>Pisciculture</term>
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<front><div type="abstract" xml:lang="en">Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris-sucrose-KCI (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris-Nacl (10 mM Tris, 25 mM NaCI, pH 8.5), and Tris-sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris-sucrose-KCI, Tris-NaCI, and Tris-sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris-sucrose-KCl and Tris-NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.</div>
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Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

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